公开(公告)号：US5527703A

公开(公告)日：1996-06-18

申请号：US249112

申请日：1994-05-25

申请人： Doris F. Cully ,  Joseph P. Arena ,  Ken K. Liu ,  Demetrios Vassilatis

发明人： Doris F. Cully ,  Joseph P. Arena ,  Ken K. Liu ,  Demetrios Vassilatis

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K39/395 ,  A61K45/00 ,  A61K48/00 ,  A61P31/00 ,  A61P43/00 ,  C07K14/435 ,  C07K14/705 ,  C07K16/18 ,  C12N5/00 ,  C12N5/10 ,  C12N15/09 ,  C12P21/02 ,  C12Q1/02 ,  C12R1/91 ,  G01N33/15 ,  C12N1/20 ,  C07H21/04 ,  C12N15/00

CPC分类号： C07K14/70571 ,  A61K38/00 ,  C12N2799/026

摘要： DNAs encoding glutamate and avermectin-sensitive chloride channels have been cloned and characterized. The individual alpha and beta subunits are capable of forming homomeric and heteromeric channels selectively opened with either avermectin or glutamate. The cDNA's have been expressed in recombinant host cells which produce active recombinant protein. The recombinant protein is also purified from the recombinant host cells. In addition, the recombinant host cells are utilized to establish a method for identifying modulators of the receptor activity, and receptor modulators are identified. Receptor modulators are useful as insecticides and anthelminthic agents.

摘要翻译： 已经克隆并表征了编码谷氨酸和除虫菌素敏感性氯通道的DNA。 单个α和β亚基能够形成与阿维菌素或谷氨酸选择性打开的同源异源通道。 cDNA已经在产生活性重组蛋白的重组宿主细胞中表达。 重组蛋白也从重组宿主细胞中纯化。 此外，重组宿主细胞用于建立识别受体活性调节剂的方法，并鉴定受体调节剂。 受体调节剂可用作杀虫剂和anthelminthic剂。