公开(公告)号：US10822637B2

公开(公告)日：2020-11-03

申请号：US15764967

申请日：2016-09-29

Applicant: DYNAMIC BIOSENSORS GMBH

Inventor： Paul Hampel ,  Johannes Reusch ,  Ulrich Rant ,  Ralf Strasser

IPC: C12Q1/6804 ,  G01N21/64 ,  G01N33/543 ,  C12Q1/6825 ,  G01N33/68 ,  G01N33/58 ,  G01N27/327

Abstract: The present invention relates to a method for detecting and/or characterizing molecular interactions between two molecules, in particular two proteins and most particular between an antigen and an antibody by using an immobilized single-stranded nucleic acid molecule to which single-stranded nucleic acid molecules having a ligand attached thereto are hybridized.

公开(公告)号：US11549939B2

公开(公告)日：2023-01-10

申请号：US16624793

申请日：2018-06-21

Applicant: Dynamic Biosensors GmbH

Inventor： Hanna Beike ,  Herwin Daub ,  Ismajli Fjolla ,  Andreas Langer ,  Ulrich Rant ,  Ralf Strasser

IPC: G01N33/542 ,  G01N21/64 ,  C12Q1/6825 ,  C12Q1/6834

Abstract: A method for characterizing the interaction between a protein and a small molecule by detecting a change in fluorescence emitted by a fluorescent dye and a nucleic acid structure which can be used in said method.

公开(公告)号：US10421997B2

公开(公告)日：2019-09-24

申请号：US15121220

申请日：2015-02-03

Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC. ,  DYNAMIC BIOSENSORS GMBH

Inventor： Dieter Heindl ,  Andreas Langer ,  Ulrich Rant ,  Ralf Strasser ,  Michael Schraeml

IPC: C12Q1/6869 ,  G01N27/327

Abstract: The present invention relates to a method of identifying a nucleotide at a defined position and determining the sequence of a target polynucleotide using an electro-switchable biosensor, as well as devices comprising an electro-switchable biosensor and uses thereof.

公开(公告)号：US10081834B2

公开(公告)日：2018-09-25

申请号：US14763078

申请日：2014-01-22

Applicant: DYNAMIC BIOSENSORS GMBH

Inventor： Andreas Langer ,  Ralf Strasser ,  Ulrich Rant

IPC: C12Q1/68 ,  C12Q1/6869 ,  C07H21/00 ,  C12Q1/6825 ,  C12Q1/6834

CPC classification number: C12Q1/6869 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q2565/607

Abstract: The present invention is directed to sequencing of nucleic acids. A method is provided for sequencing based on immobilized nucleic acid on a surface. Advantageously, a long range detection mechanism is used for detecting, whether a nucleotide provided to the substrate of a biochip has been incorporated into the immobilized template nucleic acid. Various different alignment means are provided by the present invention which can be used for facilitating a rigidly locking of the orientation of the DNA complex, which complex comprises the template nucleic acid, the primer and the capture nucleic acid. Various different linker systems may be used to immobilize the DNA complex at a first and a second strand end, such that the desired alignment of the DNA complex is achieved. Also co-adsorbed molecules on the substrate surface can be used for such an aligning measure. Additionally, or alternatively, an electrical field may be applied for repelling the DNA complex from the electrode and for facilitating a vertical DNA complex orientation. Advantageously, label-free nucleotides can be used, if desired.

公开(公告)号：US20170009285A1

公开(公告)日：2017-01-12

申请号：US15121220

申请日：2015-02-03

Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC. ,  DYNAMIC BIOSENSORS GMBH

Inventor： Dieter Heindl ,  Andreas Langer ,  Ulrich Rant ,  Ralf Strasser ,  Michael Schraeml

IPC: C12Q1/68 ,  G01N27/327

Abstract: The present invention relates to a method of identifying a nucleotide at a defined position and determining the sequence of a target polynucleotide using an electro-switchable biosensor, as well as devices comprising an electro-switchable biosensor and uses thereof.

Abstract translation: 本发明涉及一种在确定的位置鉴定核苷酸并使用可电切换生物传感器确定靶多核苷酸的序列的方法，以及包括可电切换生物传感器及其用途的装置。