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    Method for sequencing a template nucleic acid immobilized on a substrate
    4.
    发明授权
    Method for sequencing a template nucleic acid immobilized on a substrate 有权

    公开(公告)号：US10081834B2

    公开(公告)日：2018-09-25

    申请号：US14763078

    申请日：2014-01-22

    Applicant: DYNAMIC BIOSENSORS GMBH

    Inventor： Andreas Langer ,  Ralf Strasser ,  Ulrich Rant

    IPC: C12Q1/68 ,  C12Q1/6869 ,  C07H21/00 ,  C12Q1/6825 ,  C12Q1/6834

    CPC classification number: C12Q1/6869 ,  C12Q1/6825 ,  C12Q1/6834 ,  C12Q2565/607

    Abstract: The present invention is directed to sequencing of nucleic acids. A method is provided for sequencing based on immobilized nucleic acid on a surface. Advantageously, a long range detection mechanism is used for detecting, whether a nucleotide provided to the substrate of a biochip has been incorporated into the immobilized template nucleic acid. Various different alignment means are provided by the present invention which can be used for facilitating a rigidly locking of the orientation of the DNA complex, which complex comprises the template nucleic acid, the primer and the capture nucleic acid. Various different linker systems may be used to immobilize the DNA complex at a first and a second strand end, such that the desired alignment of the DNA complex is achieved. Also co-adsorbed molecules on the substrate surface can be used for such an aligning measure. Additionally, or alternatively, an electrical field may be applied for repelling the DNA complex from the electrode and for facilitating a vertical DNA complex orientation. Advantageously, label-free nucleotides can be used, if desired.

    Apparatus and Method for Evaluating Characteristics of Target Molecules
    6.
    发明申请
    Apparatus and Method for Evaluating Characteristics of Target Molecules 有权
    用于评估目标分子特征的装置和方法

    公开(公告)号：US20160011148A1

    公开(公告)日：2016-01-14

    申请号：US14856852

    申请日：2015-09-17

    Applicant: Dynamic Biosensors GmbH

    Inventor： Ulrich Rant ,  Wolfgang Kaiser ,  Paul Andreas Hampel ,  Jens Niemax ,  Andreas Langer ,  Jelena Knezevic

    IPC: G01N27/447

    CPC classification number: G01N27/44726 ,  C12Q1/6823 ,  C12Q1/6825 ,  G01N21/6408 ,  G01N21/6458 ,  G01N27/327 ,  G01N27/44756 ,  G01N33/5438 ,  G01N2021/6432 ,  C12Q2537/165 ,  C12Q2561/12 ,  C12Q2563/116

    Abstract: Arrangements are described for evaluating characteristics of target molecules. A biochip is received which includes a substrate to which charged probe molecules are attached. The probe molecules have a marker to allow generating signals indicative of the distance of a portion of the probe molecule from the substrate. The signals are detected and means for an external electric field is generated to which the probe molecules are exposed. A control means acts to: (A) apply an external electric field causing the portion of the probe molecule to approach the substrate, and (B) apply an external electric field causing the portion of the probe molecule to move away from the substrate. The signal is recorded as a function of time during step (A) and/or step (B). Steps (A) and (B) are repeated for a predetermined number of times and the recorded signals are combined.

    Abstract translation: 描述了评估靶分子特征的安排。 接收生物芯片，其包括附着有带电探针分子的基底。 探针分子具有标记以允许产生指示探针分子的一部分与基底的距离的信号。 检测信号，产生探针分子所暴露的外部电场的装置。 控制装置用于：（A）施加外部电场，使得探针分子的该部分接近衬底，和（B）施加外部电场，使得探针分子的该部分离开衬底。 在步骤（A）和/或步骤（B）期间，该信号被记录为时间的函数。 重复步骤（A）和（B）预定次数并组合记录信号。

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