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公开(公告)号：US09605292B2
公开(公告)日：2017-03-28
申请号：US15172625
申请日：2016-06-03
CPC分类号： C12P19/18 , C08B37/0009 , C12N9/1051 , C12P19/04 , C12Y204/01
摘要： A process for producing poly alpha-1,3-glucan with reduced molecular weight is disclosed. The process comprises contacting water, sucrose, a polar organic solvent, and a glucosyltransferase enzyme in a solution to produce poly alpha-1,3-glucan. This contacting step results in the production of poly alpha-1,3-glucan having a reduced molecular weight compared to the molecular weight of a poly alpha-1,3-glucan made in the absence of the polar organic solvent.
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公开(公告)号：US09296997B2
公开(公告)日：2016-03-29
申请号：US14490869
申请日：2014-09-19
发明人： Mark S Payne , Yefim Brun , Hongxian He , Thomas Scholz
CPC分类号： C12P19/18 , C08B37/0009 , C12N9/1048 , C12N9/1051 , C12P19/04 , C12Y204/01005 , C12Y204/01267 , Y02P20/52
摘要： Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.
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公开(公告)号：US09284539B2
公开(公告)日：2016-03-15
申请号：US14490706
申请日：2014-09-19
发明人： Mark S Payne , Yefim Brun , Hongxian He , Thomas Scholz
CPC分类号： C12P19/18 , C08B37/0009 , C12N9/1048 , C12N9/1051 , C12P19/04 , C12Y204/01005 , C12Y204/01267 , Y02P20/52
摘要： Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.
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4.发明授权Process for producing alpha-1,3-glucan polymer with reduced molecular weight 有权制备具有降低分子量的α-1,3-葡聚糖聚合物的方法公开(公告)号：US09169506B2
公开(公告)日：2015-10-27
申请号：US14476790
申请日：2014-09-04
CPC分类号： C12P19/18 , C08B37/0009 , C12N9/1051 , C12P19/04 , C12Y204/01
摘要： A process for producing poly alpha-1,3-glucan with reduced molecular weight is disclosed. The process comprises contacting water, sucrose, a polar organic solvent, and a glucosyltransferase enzyme in a solution to produce poly alpha-1,3-glucan. This contacting step results in the production of poly alpha-1,3-glucan having a reduced molecular weight compared to the molecular weight of a poly alpha-1,3-glucan made in the absence of the polar organic solvent.
摘要翻译： 公开了一种生产分子量降低的聚α-1,3-葡聚糖的方法。 该方法包括在溶液中使水，蔗糖，极性有机溶剂和葡糖基转移酶接触以产生聚α-1,3-葡聚糖。 该接触步骤导致与不存在极性有机溶剂时制成的聚α-1,3-葡聚糖的分子量相比，分子量降低的聚α-1,3-葡聚糖的生产。
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公开(公告)号：US10266861B2
公开(公告)日：2019-04-23
申请号：US14967659
申请日：2015-12-14
发明人： Kevin D Nagy , Susan Marie Hennessey , Mark S Payne , Charles R Powley , Bogdan Szostek , Hannu Koivikko , Juho Jarvinen , Daniel Eicholtz , Jyrki Kuusisto , Tapio Timo Viljava , Stephen Thomas Breske
摘要： Disclosed herein are aqueous compositions comprising fructose and methods of production thereof. A method for producing an aqueous composition comprising fructose can comprise, for example, conducting an enzymatic reaction by contacting water, sucrose, and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan having at least 30% alpha-1,3-linkages. A soluble fraction produced by such a reaction comprises at least about 55% fructose on a dry weight basis, and can be separated from insoluble poly alpha-1,3-glucan product(s), thereby providing an aqueous composition comprising fructose.
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公开(公告)号：US20160273013A1
公开(公告)日：2016-09-22
申请号：US15172625
申请日：2016-06-03
CPC分类号： C12P19/18 , C08B37/0009 , C12N9/1051 , C12P19/04 , C12Y204/01
摘要： A process for producing poly alpha-1,3-glucan with reduced molecular weight is disclosed. The process comprises contacting water, sucrose, a polar organic solvent, and a glucosyltransferase enzyme in a solution to produce poly alpha-1,3-glucan. This contacting step results in the production of poly alpha-1,3-glucan having a reduced molecular weight compared to the molecular weight of a poly alpha-1,3-glucan made in the absence of the polar organic solvent.
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公开(公告)号：US09296996B2
公开(公告)日：2016-03-29
申请号：US14490703
申请日：2014-09-19
发明人： Mark S Payne , Yefim Brun , Hongxian He , Thomas Scholz
CPC分类号： C12P19/18 , C08B37/0009 , C12N9/1048 , C12N9/1051 , C12P19/04 , C12Y204/01005 , C12Y204/01267 , Y02P20/52
摘要： Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.
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公开(公告)号：US09260702B2
公开(公告)日：2016-02-16
申请号：US14490844
申请日：2014-09-19
发明人： Mark S Payne , Yefim Brun , Hongxian He , Thomas Scholz
CPC分类号： C12P19/18 , C08B37/0009 , C12N9/1048 , C12N9/1051 , C12P19/04 , C12Y204/01005 , C12Y204/01267 , Y02P20/52
摘要： Reaction solutions are disclosed herein comprising water, sucrose and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan. The glucosyltransferase enzyme can synthesize insoluble glucan polymer having at least 50% alpha-1,3 glycosidic linkages and a number average degree of polymerization of at least 100. Further disclosed are methods of using such glucosyltransferase enzymes to produce insoluble poly alpha-1,3-glucan.
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公开(公告)号：US09988610B2
公开(公告)日：2018-06-05
申请号：US15182778
申请日：2016-06-15
发明人： Mark S Payne , Yefim Brun , Richard R Bott
CPC分类号： C12N9/1051 , C08B37/0009 , C12N15/102 , C12P19/04 , C12P19/18 , C12Q1/48 , C12Y204/01027
摘要： Glucosyltransferase enzymes are disclosed herein that produce branched alpha-glucan polymer. Also disclosed, for example, are polynucleotides encoding these enzymes, as well as methods of producing branched alpha-glucan polymer.
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公开(公告)号：US20170166938A1
公开(公告)日：2017-06-15
申请号：US14967659
申请日：2015-12-14
发明人： KEVIN D. NAGY , Susan Marie Hennessey , Mark S Payne , Charles R Powley , Bogdan Szostek , Hannu Koivikko , Juho Jarvinen , Daniel Eicholtz , Jyrki Kuusisto , Tapio Timo Viljava , Stephen Thomas Breske
CPC分类号： C12P19/02 , A23L1/09 , A23L29/30 , A23V2002/00 , C07H1/06 , C07H3/02 , C12P19/14 , C12P19/18 , C13K11/00
摘要： Disclosed herein are aqueous compositions comprising fructose and methods of production thereof. A method for producing an aqueous composition comprising fructose can comprise, for example, conducting an enzymatic reaction by contacting water, sucrose, and a glucosyltransferase enzyme that synthesizes poly alpha-1,3-glucan having at least 30% alpha-1,3-linkages. A soluble fraction produced by such a reaction comprises at least about 55% fructose on a dry weight basis, and can be separated from insoluble poly alpha-1,3-glucan product(s), thereby providing an aqueous composition comprising fructose.
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