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1.发明申请公开(公告)号：US20170342431A1
公开(公告)日：2017-11-30
申请号：US15673609
申请日：2017-08-10
发明人： WILLIAM JAMES GORDON-KAMM , THEODORE MITCHELL KLEIN , KEITH S. LOWE , KEVIN E MCBRIDE , CHRISTOPHER JAY SCELONGE , NING WANG
IPC分类号： C12N15/82
CPC分类号： C12N15/8241 , C12N15/8213 , C12N15/8216 , C12N15/8262
摘要： Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
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公开(公告)号：US20180171346A1
公开(公告)日：2018-06-21
申请号：US15890698
申请日：2018-02-07
发明人： WILLIAM J. GORDON-KAMM , KEITH S. LOWE , DAVID J. PETERSON , CHRISTOPHER J. SCELONGE , GRACE M. ST. CLAIR , BING-BING WANG
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8213 , C07K14/415
摘要： A variety of methods and compositions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.
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公开(公告)号：US20230203516A1
公开(公告)日：2023-06-29
申请号：US17818060
申请日：2022-08-08
发明人： TIM FOX , WILLIAM JAMES GORDON-KAMM , RACHEL CAROL HUEGEL , KEITH S. LOWE , JON AARON TUCKER REINDERS , HUAXUN YE
CPC分类号： C12N15/823 , A01H1/08 , C12N9/22 , C12N2310/20
摘要： Plant cell fate and development is altered by treating cells with cellular reprogramming factors. Embryogenesis inducing morphogenic developmental genes are used as cellular reprogramming factors, specifically comprising polypeptides or polynucleotides encoding gene products for generating doubled haploids or haploid plants from gametes. Maize microspores treated by contacting the isolated cells with an exogenous purified, recombinant embryogenesis inducing morphogenic developmental gene polypeptide results in embryogenesis. The gametes of a maize plant develop into embryoids when transformed with a genetic construct including regulatory elements and structural genes capable of acting in a cascading fashion to alter cellular fate of plant cells. Developmental morphogenic proteins expressed from a genetic construct are used for ex situ treatment methods and for in planta cellular reprogramming.
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公开(公告)号：US20180371480A1
公开(公告)日：2018-12-27
申请号：US16062646
申请日：2016-08-26
发明人： ALEXANDRE DA SILVA CONCEICAO , WILLIAM JAMES GORDON-KAMM , THEODORE M. KLEIN , CARLOS M. LA ROTA , KEITH S. LOWE
IPC分类号： C12N15/82
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for regulatory regions. Phospholipid transfer protein (PLTP) promoters are provided. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence, such as a PLTP promoter, disclosed herein. DNA constructs comprising a promoter operably linked to a heterologous nucleotide sequence of interest are also provided.
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5.发明申请METHODS AND COMPOSITIONS FOR THE INTRODUCTION AND REGULATED EXPRESSION OF GENES IN PLANTS 有权用于植物基因引入和调控表达的方法和组合物公开(公告)号：US20140157453A1
公开(公告)日：2014-06-05
申请号：US14087775
申请日：2013-11-22
发明人： WILLIAM JAMES GORDON-KAMM , THEODORE MITCHELL KLEIN , KEITH S. LOWE , KEVIN E. MCBRIDE , CHRISTOPHER JAY SCELONGE , NING WANG
IPC分类号： C12N15/82
CPC分类号： C12N15/8241 , C12N15/8213 , C12N15/8216 , C12N15/8262
摘要： Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
摘要翻译： 为植物中基因的引入和调控表达提供了组合物和方法。 组合物包括提供可用于调节表达位点特异性重组酶的活性水平的启动子构建体，同时避免过早切除。 进一步提供了编码新型婴儿多肽，表达盒和包含其的植物的分离的多核苷酸。 提供了将基因导入植物的方法，包括质体转化的方法和从成熟种子和叶子转化组织的方法。
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公开(公告)号：US20200032281A1
公开(公告)日：2020-01-30
申请号：US16540889
申请日：2019-08-14
发明人： WILLIAM JAMES GORDON-KAMM , KEITH S. LOWE , DAVID J. PETERSON , CHRISTOPHER J. SCELONGE , GRACE M. ST CLAIR , BING-BING WANG
IPC分类号： C12N15/82 , C07K14/415
摘要： A variety of methods and compostions are provided, including methods and compositions for targeted modification of a specific target site in a cell or organism, methods for integrating polynucleotides of interest, methods to assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, silence a gene, and identify and/or characterize transcriptional regulating regions. The methods involve the introduction of a cell proliferation factor and a double-strand break-inducing enzyme into an organism.
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公开(公告)号：US20190100765A1
公开(公告)日：2019-04-04
申请号：US16143635
申请日：2018-09-27
发明人： WILLIAM J. GORDON-KAMM , TIMOTHY G. HELENTJARIS , KEITH S. LOWE , BO SHEN , MITCHELL C. TARCZYNSKI , PEIZHONG ZHENG
IPC分类号： C12N15/82 , A01H1/08 , C07K14/415
摘要： Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
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公开(公告)号：US20190017061A1
公开(公告)日：2019-01-17
申请号：US16142766
申请日：2018-09-26
发明人： WILLIAM J. GORDON-KAMM , TIMOTHY G. HELENTJARIS , KEITH S. LOWE , BO SHEN , MITCHELL C. TARCZYNSKI , PEIZHONG ZHENG
IPC分类号： C12N15/82 , C07K14/415 , A01H1/08
摘要： Methods and compositions for modulating plant development are provided. Nucleotide sequences and amino acid sequences encoding Ovule Development Protein 2 (ODP2) proteins are provided. The sequences can be used in a variety of methods including modulating development, developmental pathways, altering oil content in a plant, increasing transformation efficiencies, modulating stress tolerance, and modulating the regenerative capacity of a plant. Transformed plants, plant cells, tissues, and seed are also provided.
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公开(公告)号：US20170121722A1
公开(公告)日：2017-05-04
申请号：US15249318
申请日：2016-08-26
发明人： AJITH ANAND , MAREN L. ARLING , ALEXANDRE DA SILVA CONCEICAO , WILLIAM JAMES GORDON-KAMM , CRAIG E. HASTINGS , GEORGE M. HOERSTER , THEODORE MITCHELL KLEIN , CARLOS M. LA ROTA , KEITH S. LOWE , SHIV BAHADUR TIWARI , NING WANG , XINLI EMILY WU
IPC分类号： C12N15/82 , C07K14/415 , A01H4/00
CPC分类号： C12N15/8213 , A01H4/008 , C07K14/415 , C12N15/8201 , C12N15/821 , C12N15/8216
摘要： The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
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