公开(公告)号：US20140157453A1

公开(公告)日：2014-06-05

申请号：US14087775

申请日：2013-11-22

申请人： E.I. DUPONT DE NEMOURS ,  PIONEER HI BRED INTERNATIONAL INC

发明人： WILLIAM JAMES GORDON-KAMM ,  THEODORE MITCHELL KLEIN ,  KEITH S. LOWE ,  KEVIN E. MCBRIDE ,  CHRISTOPHER JAY SCELONGE ,  NING WANG

IPC分类号： C12N15/82

CPC分类号： C12N15/8241 ,  C12N15/8213 ,  C12N15/8216 ,  C12N15/8262

摘要： Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.

摘要翻译： 为植物中基因的引入和调控表达提供了组合物和方法。 组合物包括提供可用于调节表达位点特异性重组酶的活性水平的启动子构建体，同时避免过早切除。 进一步提供了编码新型婴儿多肽，表达盒和包含其的植物的分离的多核苷酸。 提供了将基因导入植物的方法，包括质体转化的方法和从成熟种子和叶子转化组织的方法。

公开(公告)号：US20190292558A1

公开(公告)日：2019-09-26

申请号：US16433131

申请日：2019-06-06

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： ERICKA BERMUDEZ ,  LINDA A. CASTLE ,  KEVIN E. MCBRIDE ,  DANIEL SIEHL

IPC分类号： C12N15/82 ,  C12N9/10

摘要： Compositions and methods comprising polynucleotides and polypeptides having glyphosate-N-acetyltransferase (GLYAT) activity are provided. In specific embodiments, the sequence has an improved property, such as, but not limited to, an improved specificity for glyphosate when compared to an appropriate control resulting in decreased off target acetylation of, e.g. an amino acid such as aspartate. Further provided are nucleic acid constructs, plants, plant cells, explants, seeds and grain having the GLYAT sequences. Various methods of employing the GLYAT sequences are provided. Such methods include methods for producing a glyphosate tolerant plant, plant cell, explant or seed and methods of controlling weeds in a field containing a crop employing the plants and/or seeds disclosed herein.

公开(公告)号：US20180216123A1

公开(公告)日：2018-08-02

申请号：US15756023

申请日：2016-08-26

申请人： PIONEER HI-BRED INTERNATIONAL, INC. ,  E. I. DU PONT DE NEMOURS AND COMPANY

发明人： AJITH ANAND ,  STEVEN HENRY BASS ,  SEAN M. BERTAIN ,  HYEON-JE CHO ,  ANTHONY J. KINNEY ,  THEODORE M. KLEIN ,  MICHAEL LASSNER ,  KEVIN E. MCBRIDE ,  YORK MOY ,  BARBARA ANN MARIE ROSEN ,  JUN-ZHI WEI

IPC分类号： C12N15/82 ,  C12N15/65

CPC分类号： C12N15/8205 ,  C07K14/195 ,  C12N15/65 ,  C12N15/8202 ,  C12N15/8243

摘要： Methods and compositions for Ochrobactrum-mediated transformation of plants are provided. Methods include but are not limited to using an Ochrobactrum strain to transfer a polynucleotide of interest to a plant cell. These include VirD2-dependent methods. Compositions include an Ochrobactrum strain, transfer DNAs, constructs and/or plasmids. These include Ochrobactrum strains having a plasmid comprising one or more virulence gene(s), border region, and/or origin of replication. Plant cells, tissues, plants, and seeds comprising a polynucleotide of interest produced by the methods are also provided.

公开(公告)号：US20190078106A1

公开(公告)日：2019-03-14

申请号：US15765521

申请日：2016-08-26

申请人： PIONEER HI-BRED INTERNATIONAL, INC.

发明人： AJITH ANAND ,  STEVEN HENRY BASS ,  HYEON-JE CHO ,  THEODORE MITCHELL KLEIN ,  MICHAEL LASSNER ,  KEVIN E. MCBRIDE

IPC分类号： C12N15/82 ,  C07K14/245

摘要： The present disclosure provides methods and compositions for the vectors comprising vir genes. The present disclosure further provides a vector comprising: (a) an origin of replication for propagation and stable maintenance in Escherichia coli; (b) an origin of replication for propagation and stable maintenance in Agrobacterium spp.; (c) a selectable marker gene; and (d) Rhizobiaceae virulence genes virB1-B11 or r-virB1-B11, virC1-C2 or r-virC1-C2, virD1-D2 or r-virD1-D2, and virG or r-virG, or variants and derivatives thereof, wherein the vector comprising the virulence genes r-virB1-B11, r-virC1-C2, r-virD1-D2, and r-virG further comprises a r-galls virulence gene, or variants and derivatives thereof. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.

公开(公告)号：US20140033371A1

公开(公告)日：2014-01-30

申请号：US14045087

申请日：2013-10-03

申请人： PIONEER HI-BRED INTERNATIONAL INC ,  E I DU PONT DE NEMOURS AND COMPANY

发明人： KEVIN E. MCBRIDE ,  MICHAEL W. LASSNER ,  LOREN L. LOOGER ,  BRIAN MCGONIGLE

IPC分类号： C12N15/63 ,  C07K14/245

CPC分类号： C07K14/245 ,  C07K14/195 ,  C12N15/635 ,  C12N15/8217 ,  C12N15/8238 ,  C12N15/8278

摘要： Compositions and methods relating to the use of sulfonylurea-responsive repressors are provided. Compositions include polypeptides that specifically bind to an operator, wherein the specific binding is regulated by a sulfonylurea compound. Compositions also include polynucleotides encoding the polypeptides as well as constructs, vectors, prokaryotic and eukaryotic cells, and eukaryotic organisms including plants and seeds comprising the polynucleotide, and/or produced by the methods. Also provided are methods to provide a sulfonylurea-responsive repressor to a cell or organism, and to regulate expression of a polynucleotide of interest in a cell or organism, including a plant or plant cell.

摘要翻译： 提供了与使用磺酰脲类阻遏物相关的组合物和方法。 组合物包括特异性结合操作者的多肽，其中特异性结合受磺酰脲化合物调节。 组合物还包括编码多肽的多核苷酸以及构建体，载体，原核和真核细胞，以及真核生物，包括包含多核苷酸的植物和种子，和/或由该方法产生的。 还提供了向细胞或生物体提供磺酰脲反应性阻遏物并调节目标多核苷酸在包括植物或植物细胞的细胞或生物体中的表达的方法。