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1.发明申请METHODS AND COMPOSITIONS FOR THE INTRODUCTION AND REGULATED EXPRESSION OF GENES IN PLANTS 有权用于植物基因引入和调控表达的方法和组合物公开(公告)号：US20140157453A1
公开(公告)日：2014-06-05
申请号：US14087775
申请日：2013-11-22
发明人： WILLIAM JAMES GORDON-KAMM , THEODORE MITCHELL KLEIN , KEITH S. LOWE , KEVIN E. MCBRIDE , CHRISTOPHER JAY SCELONGE , NING WANG
IPC分类号： C12N15/82
CPC分类号： C12N15/8241 , C12N15/8213 , C12N15/8216 , C12N15/8262
摘要： Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
摘要翻译： 为植物中基因的引入和调控表达提供了组合物和方法。 组合物包括提供可用于调节表达位点特异性重组酶的活性水平的启动子构建体，同时避免过早切除。 进一步提供了编码新型婴儿多肽，表达盒和包含其的植物的分离的多核苷酸。 提供了将基因导入植物的方法，包括质体转化的方法和从成熟种子和叶子转化组织的方法。
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公开(公告)号：US20210285006A1
公开(公告)日：2021-09-16
申请号：US17195881
申请日：2021-03-09
IPC分类号： C12N15/82
摘要： Automated, high-throughput and multiplexed methods for clonal plant production from maternal or paternal derived cellular structures including embryos and microspores, using morphogenic factors to produce plants are disclosed. Large scale and rapid clonal propagation methods facilitate increased efficiency in producing doubled haploid plants for breeding and for trait introgression purposes.
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3.发明申请公开(公告)号：US20210238614A1
公开(公告)日：2021-08-05
申请号：US17053663
申请日：2019-05-07
IPC分类号： C12N15/82
摘要： Methods and compositions are provided for the improvement of homology-directed repair of a double strand break in a plant cell, via the use of a polynucleotide comprising sequences homologous to the target site. In some aspects, the double strand break is created by an RNA-guided Cas endonuclease. The homology-directed repair of the double-strand break may include incorporation of a heterologous polynucleotide, for example a gene encoding a trait of agronomic importance. The homology-directed repair of the double-strand break may occur as a result of template-directed repair using a polynucleotide repair template.
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公开(公告)号：US20230407320A1
公开(公告)日：2023-12-21
申请号：US18250469
申请日：2021-10-27
CPC分类号： C12N15/8205 , C12N2310/20 , C12N9/22
摘要： Methods and compositions are provided for the transient expression and activity of site-specific DNA modifying agent, morphogenic factors or developmental genes, either alone or in combination for eukaryotic cells. The morphogenic factor and DNA modifying agent may be provided to the same cell or to a different cell than that which was originally transformed. The morphogenic factor, or the double-strand-break-inducing agent, may further comprise a cell penetrating peptide. Exogenously provided DNA modifying agents and/or morphogenic factors need not be segregated away in future generations due to transient activities in the desired cell.
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公开(公告)号：US20220170033A1
公开(公告)日：2022-06-02
申请号：US17442552
申请日：2020-03-26
发明人： MAREN L ARLING , SHUJUN CHANG , WILLIAM JAMES GORDON-KAMM , NATHALIE SANYOUR-DOYEL , NAGESH SARDESAI
IPC分类号： C12N15/82 , C07K14/415 , C12N9/22
摘要： Methods for transforming dicot vegetative plant organs and their composite tissues are provided.
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公开(公告)号：US20220124998A1
公开(公告)日：2022-04-28
申请号：US17571428
申请日：2022-01-07
发明人： AJITH ANAND , MAREN L ARLING , ALEXANDRE DA SILVA CONCEICAO , WILLIAM JAMES GORDON-KAMM , CRAIG HASTINGS , GEORGE J HOERSTER , TODD J JONES , THEODORE M KLEIN , CARLOS M LA ROTA , KEITH S LOWE , SHIV BAHADUR TIWARI , NING WANG , XINLI EMILY WU
摘要： The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
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7.发明申请公开(公告)号：US20210180077A1
公开(公告)日：2021-06-17
申请号：US17181136
申请日：2021-02-22
发明人： WILLIAM JAMES GORDON-KAMM , THEODORE M KLEIN , KEITH S LOWE , KEVIN E MCBRIDE , CHRISTOPHER J SCELONGE , NING WANG
IPC分类号： C12N15/82
摘要： Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
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公开(公告)号：US20180265877A1
公开(公告)日：2018-09-20
申请号：US15765026
申请日：2016-10-11
CPC分类号： C12N15/65 , A01H1/04 , A01H4/00 , C12N15/743 , C12N15/8205 , C12N15/8209
摘要： The present disclosure comprises methods and compositions comprising a plant transforming bacterium of the Order Rhizobiales comprising conditional negative selectable marker genes.
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公开(公告)号：US20180119162A1
公开(公告)日：2018-05-03
申请号：US15554234
申请日：2016-03-16
CPC分类号： C12N15/8241 , A01H1/04 , C12N15/8212 , C12N15/8213 , C12N15/825 , C12N15/8289
摘要： Compositions and methods are provided for the use of pollen-inhibitor genes and/or color marker genes in accelerated trait introgression. Compositions and methods are also provided for introducing a pollen-inhibitor gene and/or a color marker gene in close proximity to a trait locus of interest. Breeding methods and methods for selecting plants comprising a trait locus of interest in close proximity to at least one pollen-inhibitor gene and/or color marker gene are also disclosed. The methods and compositions employ at least one pollen-inhibitor gene and/or color marker gene to provide an effective system for accelerated trait introgression in the genome of a plant.
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公开(公告)号：US20240002870A1
公开(公告)日：2024-01-04
申请号：US18246899
申请日：2021-09-28
发明人： AJITH ANAND , WILLIAM JAMES GORDON-KAMM , LARISA A. RYAN , NAGESH SARDESAI , NING WANG , HUIXIA WU , XINLI EMILY WU
IPC分类号： C12N15/82
CPC分类号： C12N15/8225 , C12N15/8205
摘要： Methods for transforming monocot leaf explants are provided.
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