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1.发明授权公开(公告)号：US10968458B2
公开(公告)日：2021-04-06
申请号：US15673609
申请日：2017-08-10
发明人： William James Gordon-Kamm , Theodore Mitchell Klein , Keith S. Lowe , Kevin E. McBride , Christopher Jay Scelonge , Ning Wang
IPC分类号： C12N15/82
摘要： Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
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公开(公告)号：US20140137291A1
公开(公告)日：2014-05-15
申请号：US14169237
申请日：2014-01-31
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8247 , C07K14/415 , C12N15/8261 , Y02A40/146
摘要： This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.
摘要翻译： 本发明涉及编码转录因子的分离的核酸片段。 本发明还涉及在正义或反义方向上编码全部或部分转录因子的嵌合基因的构建，其中嵌合基因的表达导致转化的宿主细胞中积累的油的水平的改变。
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公开(公告)号：US08692072B2
公开(公告)日：2014-04-08
申请号：US13905588
申请日：2013-05-30
IPC分类号： A01H1/00 , C12N15/63 , C07H21/04 , C07K14/415 , C12N15/00
CPC分类号： C12N15/8247 , C07K14/415 , C12N15/8261 , Y02A40/146
摘要： This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.
摘要翻译： 本发明涉及编码转录因子的分离的核酸片段。 本发明还涉及在正义或反义方向上编码全部或部分转录因子的嵌合基因的构建，其中嵌合基因的表达导致转化的宿主细胞中积累的油的水平的改变。
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公开(公告)号：US20130276167A1
公开(公告)日：2013-10-17
申请号：US13905588
申请日：2013-05-30
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8247 , C07K14/415 , C12N15/8261 , Y02A40/146
摘要： This invention relates to an isolated nucleic acid fragment encoding a transcription factor. The invention also relates to the construction of a chimeric gene encoding all or a portion of the transcription factor, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of accumulated oil in a transformed host cell.
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公开(公告)号：US11330776B2
公开(公告)日：2022-05-17
申请号：US15249318
申请日：2016-08-26
发明人： Ajith Anand , Maren L. Arling , Alexandre Da Silva Conceicao , William James Gordon-Kamm , Craig E Hastings , George J Hoerster , Todd J Jones , Theodore Mitchell Klein , Carlos M La Rota , Keith S Lowe , Shiv Bahadur Tiwari , Ning Wang , Xinli Emily Wu
IPC分类号： A01H4/00 , C12N15/82 , C07K14/415
摘要： The disclosure pertains to methods and compositions for the rapid and efficient transformation of plants. The disclosure further provides methods for producing a transgenic plant, comprising (a) transforming a cell of an explant with an expression construct comprising (i) a nucleotide sequence encoding a WUS/WOX homeobox polypeptide; (ii) a nucleotide sequence encoding a polypeptide comprising two AP2-DNA binding domains; or (iii) a combination of (i) and (ii); and (b) allowing expression of the polypeptide of (a) in each transformed cell to form a regenerable plant structure in the absence of exogenous cytokinin, wherein no callus is formed; and (c) germinating the regenerable plant structure to form the transgenic plant. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present disclosure.
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公开(公告)号：US11104911B2
公开(公告)日：2021-08-31
申请号：US16062646
申请日：2016-08-26
发明人： Alexandre Da Silva Conceicao , William James Gordon-Kamm , Theodore Mitchell Klein , Carlos M. La Rota , Keith S. Lowe
IPC分类号： C12N15/82
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences for regulatory regions. Phospholipid transfer protein (PLTP) promoters from maize are provided. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence, such as a maize PLTP promoter, disclosed herein. DNA constructs comprising a promoter operably linked to a heterologous nucleotide sequence of interest are also provided.
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7.发明授权公开(公告)号：US09765352B2
公开(公告)日：2017-09-19
申请号：US14087775
申请日：2013-11-22
发明人： William James Gordon-Kamm , Theodore Mitchell Klein , Keith S Lowe , Kevin E McBride , Christopher Jay Scelonge , Ning Wang
IPC分类号： C12N15/82
CPC分类号： C12N15/8241 , C12N15/8213 , C12N15/8216 , C12N15/8262
摘要： Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves.
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