公开(公告)号：US11060138B1

公开(公告)日：2021-07-13

申请号：US17016353

申请日：2020-09-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling Chen ,  Minghao Guo ,  Michael Previte ,  Chunhong Zhou ,  Derek Fuller

IPC: C12Q1/6869 ,  G01N15/14

Abstract: Methods and systems for sequencing a nucleic acid molecule are described that comprise imaging a first surface and an axially-displaced second surface using a compensation-free optical system, the system comprising an objective lens and at least one image sensor, wherein said optical system has a numerical aperture (NA) of less than 0.6 and a field-of-view (FOV) of greater than 1.0 mm2; and) processing the images of the first surface and the axially-displaced second surface to correct for optical aberration such that the images of the first surface and the axially-displaced second surface have substantially the same optical resolution.

公开(公告)号：US11795504B2

公开(公告)日：2023-10-24

申请号：US17574416

申请日：2022-01-12

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling Chen ,  Minghao Guo ,  Michael Previte ,  Chunhong Zhou ,  Derek Fuller

IPC: C12Q1/6869 ,  G01N21/64 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6806

CPC classification number: C12Q1/6869 ,  C12N15/1006 ,  C12Q1/6806 ,  G01N15/147 ,  G01N15/1436 ,  G01N15/1484 ,  G01N21/6402 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N21/6486 ,  G01N2015/144 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2021/6463 ,  G01N2201/0612

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

公开(公告)号：US11459608B2

公开(公告)日：2022-10-04

申请号：US17372362

申请日：2021-07-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling Chen ,  Minghao Guo ,  Michael Previte ,  Chunhong Zhou ,  Derek Fuller

IPC: C12Q1/6869 ,  G01N21/64 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6806

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

公开(公告)号：US11053540B1

公开(公告)日：2021-07-06

申请号：US17016349

申请日：2020-09-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling Chen ,  Minghao Guo ,  Michael Previte ,  Chunhong Zhou ,  Derek Fuller

IPC: C12Q1/68 ,  C12Q1/6869 ,  G01N21/64

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

公开(公告)号：US11408032B2

公开(公告)日：2022-08-09

申请号：US17016350

申请日：2020-09-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling Chen ,  Minghao Guo ,  Michael Previte ,  Chunhong Zhou ,  Derek Fuller

IPC: G01N21/64 ,  C12Q1/6869 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6806

Abstract: Imaging systems and methods comprising imaging a first interior surface and a second interior surface of a flow cell are described. In some embodiments, the imaging systems may comprise: a) an objective lens; b) at least one image sensor; and c) at least one tube lens disposed in an optical path between the objective lens and the at least one image sensor; wherein the at least one tube lens is configured to correct imaging performance such that images of the first interior surface of the flow cell and the second interior surface of the flow cell have substantially the same optical resolution.

公开(公告)号：US11365444B2

公开(公告)日：2022-06-21

申请号：US17372361

申请日：2021-07-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling Chen ,  Minghao Guo ,  Michael Previte ,  Chunhong Zhou ,  Derek Fuller

IPC: C12Q1/6869 ,  G01N21/64 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6806

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.

公开(公告)号：US11339433B2

公开(公告)日：2022-05-24

申请号：US17496710

申请日：2021-10-07

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling Chen ,  Minghao Guo ,  Michael Previte ,  Chunhong Zhou ,  Derek Fuller

IPC: C12Q1/6869 ,  G01N15/14 ,  G01N21/64 ,  C12N15/10 ,  C12Q1/6806

Abstract: Methods and systems for sequencing a nucleic acid molecule are described that comprise imaging a first surface and an axially-displaced second surface using a compensation-free optical system, the system comprising an objective lens and at least one image sensor, wherein said optical system has a numerical aperture (NA) of less than 0.6 and a field-of-view (FOV) of greater than 1.0 mm2; and) processing the images of the first surface and the axially-displaced second surface to correct for optical aberration such that the images of the first surface and the axially-displaced second surface have substantially the same optical resolution.

公开(公告)号：US11261489B2

公开(公告)日：2022-03-01

申请号：US17372359

申请日：2021-07-09

Applicant: Element Biosciences, Inc.

Inventor： Steve Xiangling Chen ,  Minghao Guo ,  Michael Previte ,  Chunhong Zhou ,  Derek Fuller

IPC: C12Q1/6869 ,  G01N21/64 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6806

Abstract: Fluorescence imaging system designs are described that provide larger fields-of-view, increased spatial resolution, improved modulation transfer and image quality, higher spatial sampling frequency, faster transitions between image capture when repositioning the sample plane to capture a series of images (e.g., of different fields-of-view), and improved imaging system duty cycle, and thus enable higher throughput image acquisition and analysis for genomics and other imaging applications.