公开(公告)号：US12163163B2

公开(公告)日：2024-12-10

申请号：US18160952

申请日：2023-01-27

Applicant: Element Biosciences, Inc.

Inventor： Jendrik Boothby-Hentschel ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger ,  Mark Ambroso

IPC: C12N9/12 ,  C12Q1/6869

Abstract: Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US12091657B2

公开(公告)日：2024-09-17

申请号：US17120030

申请日：2020-12-11

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Matthew Kellinger ,  Molly He ,  Tyler Lopez

IPC: C12N15/10 ,  C12N9/12 ,  C12P21/00 ,  C12Q1/6874

CPC classification number: C12N15/102 ,  C12N9/1276 ,  C12N15/1068 ,  C12Q1/6874 ,  C12Y207/07049 ,  C12N2310/321 ,  C12P21/00 ,  C12Q1/6874 ,  C12Q2521/107 ,  C12Q2525/186

Abstract: Provided herein are compositions and methods for the incorporation of unnatural nucleotides using mutant polymerases, such as reverse transcriptases. Further provided herein are methods of detection and sequencing of polynucleotide sequences. In some aspects, the compositions and methods are used enhance the efficiency and speed of detecting nucleotide bases. The methods and compositions described herein may further reduce time, cost, or scale of devices for next generation sequencing platforms.

公开(公告)号：US12270056B2

公开(公告)日：2025-04-08

申请号：US17705020

申请日：2022-03-25

Applicant: Element Biosciences, Inc.

Inventor： Mark Ambroso ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger

IPC: C12N9/12 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874

Abstract: Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US12139727B2

公开(公告)日：2024-11-12

申请号：US17705043

申请日：2022-03-25

Applicant: Element Biosciences, Inc.

Inventor： Mark Ambroso ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger

IPC: C12N9/12 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874

Abstract: Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US11788075B2

公开(公告)日：2023-10-17

申请号：US18160947

申请日：2023-01-27

Applicant: Element Biosciences, Inc.

Inventor： Jendrik Hentschel ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger ,  Mark Ambroso

IPC: C12N9/12 ,  C12Q1/6869

CPC classification number: C12N9/1252 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US11859241B2

公开(公告)日：2024-01-02

申请号：US18166429

申请日：2023-02-08

Applicant: Element Biosciences, Inc.

Inventor： Sinan Arslan ,  Junhua Zhao ,  Molly He ,  Samantha Snow ,  William Light ,  Matthew Kellinger ,  Michael Previte ,  Michael Kim ,  Hua Yu ,  Yu-Hsien Hwang-Fu ,  Marco Tjioe ,  Andrew Boddicker ,  Mark Ambroso ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874 ,  G01N21/64 ,  C12Q1/6853

CPC classification number: C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6874 ,  G01N21/6428 ,  G01N21/6458 ,  C12Q2600/158

Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

公开(公告)号：US20220403351A1

公开(公告)日：2022-12-22

申请号：US17705011

申请日：2022-03-25

Applicant: Element Biosciences, Inc.

Inventor： Mark Ambroso ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger

IPC: C12N9/12 ,  C12Q1/6874

Abstract: Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US12006518B2

公开(公告)日：2024-06-11

申请号：US18160951

申请日：2023-01-27

Applicant: Element Biosciences, Inc.

Inventor： Jendrik Hentschel ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger ,  Mark Ambroso

IPC: C12N9/12 ,  C12Q1/6869

CPC classification number: C12N9/1252 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US20220403353A1

公开(公告)日：2022-12-22

申请号：US17705043

申请日：2022-03-25

Applicant: Element Biosciences, Inc.

Inventor： Mark Ambroso ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger

IPC: C12N9/12 ,  C12Q1/6874

Abstract: Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US20220403352A1

公开(公告)日：2022-12-22

申请号：US17705020

申请日：2022-03-25

Applicant: Element Biosciences, Inc.

Inventor： Mark Ambroso ,  Tyler Lopez ,  Michael Klein ,  Virginia Saade ,  Matthew Kellinger

IPC: C12N9/12 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874

Abstract: Provided herein are engineered variants of archaeal, prokaryotic, and eukaryotic polymerases that exhibit enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, and improved uracil-tolerance, in polymerase-catalyzed nucleotide extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases, forming binding complexes and forming ternary complexes, and uses for conducting nucleic acid sequencing reactions.