中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

13 records (took 0.017 seconds)

    Compositions and methods for pairwise sequencing
    3.
    发明授权
    Compositions and methods for pairwise sequencing 有权

    公开(公告)号：US11535892B1

    公开(公告)日：2022-12-27

    申请号：US17377279

    申请日：2021-07-15

    Applicant: Element Biosciences, Inc.

    Inventor： Sinan Arslan ,  Junhua Zhao ,  Molly He ,  Samantha Snow ,  William Light ,  Matthew Kellinger ,  Michael Previte ,  Michael Kim ,  Hua Yu ,  Yu-Hsien Hwang-Fu ,  Marco Tjioe ,  Andrew Boddicker

    IPC: C12Q1/6853 ,  C12Q1/6874

    Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

    Compositions and methods for pairwise sequencing
    4.
    发明授权
    Compositions and methods for pairwise sequencing 有权

    公开(公告)号：US11220707B1

    公开(公告)日：2022-01-11

    申请号：US17377284

    申请日：2021-07-15

    Applicant: Element Biosciences, Inc.

    Inventor： Sinan Arslan ,  Junhua Zhao ,  Molly He ,  Samantha Snow ,  William Light ,  Matthew Kellinger ,  Michael Previte

    IPC: C12P19/34 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6874

    Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

    Compositions and methods for pairwise sequencing
    6.
    发明授权
    Compositions and methods for pairwise sequencing 有权

    公开(公告)号：US11427855B1

    公开(公告)日：2022-08-30

    申请号：US17377283

    申请日：2021-07-15

    Applicant: Element Biosciences, Inc.

    Inventor： Sinan Arslan ,  Junhua Zhao ,  Molly He ,  Samantha Snow ,  William Light ,  Matthew Kellinger ,  Michael Previte ,  Michael Kim ,  Hua Yu ,  Yu-Hsien Hwang-Fu ,  Marco Tjioe ,  Andrew Boddicker

    IPC: C12Q1/68 ,  C12Q1/6806 ,  C12Q1/6834 ,  G01N21/64 ,  C12Q1/6874

    Abstract: The present disclosure provides compositions and methods that employ the compositions for conducting pairwise sequencing and for generating concatemer template molecules for pairwise sequencing. The concatemers can be generated using a rolling circle amplification reaction which is conducted either on-support, or conducted in-solution and then distributed onto a support. The rolling circle amplification reaction generates concatemers containing tandem copies of a sequence of interest and at least one universal adaptor sequence. An increase in the number of tandem copies in a given concatemer increases the number of sites along the concatemer for hybridizing to multiple sequencing primers which serve as multiple initiation sites for polymerase-catalyzed sequencing reactions. When the sequencing reaction employs detectably labeled nucleotides and/or detectably labeled multivalent molecules (e.g., having nucleotide units), the signals emitted by the nucleotides or nucleotide units that participate in the parallel sequencing reactions along the concatemer yields an increased signal intensity for each concatemer.

    Methods and reagents for nucleic acid analysis
    10.
    发明授权
    Methods and reagents for nucleic acid analysis 有权

    公开(公告)号：US11781185B2

    公开(公告)日：2023-10-10

    申请号：US17947984

    申请日：2022-09-19

    Applicant: Element Biosciences, Inc.

    Inventor： Sinan Arslan ,  Molly He ,  Michael Previte ,  Ramreddy Tippana ,  Hua Yu ,  William Light ,  Junhua Zhao

    IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6806

    CPC classification number: C12Q1/6874 ,  C12Q1/6806

    Abstract: Provided herein are fluorescently-labeled nucleotide conjugates for nucleic acid analysis. Also provided are reagents used for forming binding complexes between a fluorescently-labeled nucleotide conjugate and a target nucleic acid sequence in the presence of one or more reagents disclosed herein. Binding complexes can be detected in the presence of the one or more reagents. For example, the one or more reagents may contain a photobleaching reducing agent configured to reduce photobleaching resulting from use of the fluorescently-labeled nucleotide conjugate to form the binding complex in a nucleic acid analysis. Such nucleic acid analysis may be used to identify sites of nucleobase binding or incorporation between the target nucleic acid sequence and one or more nucleotide moieties of the fluorescently-labeled nucleotide conjugate in a nucleic acid sequence reaction.

Patent Agency Ranking