公开(公告)号：US09067976B2

公开(公告)日：2015-06-30

申请号：US14083590

申请日：2013-11-19

Applicant: EMD Millipore Corporation

Inventor： Kwok-Shun Cheng ,  Senthilkumar Ramaswamy ,  Chen Wang ,  Nanying Bian ,  Brian Gagnon ,  Joaquin A. Umana ,  Dennis Aquino ,  Neil Soice ,  Andrew Lyddiatt

IPC: C07K16/00 ,  B01J20/26 ,  B01J20/28 ,  B01J20/285 ,  B01J20/286 ,  B01J20/32 ,  B01J39/26

CPC classification number: C07K16/00 ,  B01J20/262 ,  B01J20/265 ,  B01J20/267 ,  B01J20/28019 ,  B01J20/28092 ,  B01J20/285 ,  B01J20/286 ,  B01J20/3212 ,  B01J20/3244 ,  B01J20/3274 ,  B01J20/328 ,  B01J20/3285 ,  B01J20/3293 ,  B01J39/26 ,  Y10T428/2982

Abstract: The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.

Abstract translation: 本发明涉及适用于分离应用的非对称色谱介质，特别是作为填充床，流化床或磁化床层析介质。 在某些实施方案中，不对称色谱介质包含具有至少两个不同的，受控的孔径分布的不对称颗粒，优选珠粒。 优选地，不同孔径分布中的一个在颗粒的内部区域中，另一个位于颗粒的外部区域或涂层中。 这些不同的孔径分布可以用均匀的或替代地独特的官能团或官能团的混合物进行改性。 本发明允许通过提供不同的内部区域，优选以珠粒的形式和不同的外部区域，优选以珠粒上的涂层的形式来控制不对称多孔颗粒内的孔径分布。