公开(公告)号：US20210371835A1

公开(公告)日：2021-12-02

申请号：US17264096

申请日：2019-07-30

申请人： ERBER AKTIENGESELLSCHAFT

发明人： Michaela Thamhesl ,  Sebastian Fruhauf ,  Wulf-Dieter Moll ,  Andreas Hoebartner ,  Gerd Schatzmayr ,  Eva Maria Binder ,  Markus Aleschko ,  Martin Pfeffer

IPC分类号： C12N9/14 ,  A23K20/189 ,  A23K50/30 ,  A23K50/60 ,  A23K50/75 ,  A23L29/00 ,  A23L33/10 ,  A23L33/00 ,  A61K38/46

摘要： The present invention relates to a method for increasing the stability of an α/β-hydrolase. In addition, the present invention relates to an α/β-hydrolase obtainable by the method of the present invention. Also provided are α/β-hydrolases having a decreased grand average of hydropathy (GRAVY) value and/or comprising specific mutations. In addition, the present invention concerns a use of an α/β-hydrolase of the present invention for degrading zearalenone (ZEN).

公开(公告)号：US10264807B2

公开(公告)日：2019-04-23

申请号：US15553218

申请日：2015-02-24

申请人： ERBER AKTIENGESELLSCHAFT

发明人： Markus Aleschko ,  Corinna Kern ,  Dieter Moll ,  Eva Maria Binder ,  Gerd Schatzmayr

IPC分类号： C12N9/18 ,  C12N15/55 ,  A23K10/14 ,  A23K50/30 ,  A23K50/60 ,  A23K20/147 ,  C12N1/21

摘要： The invention relates to fusarium toxin-cleaving polypeptide variants of a fusarium toxin carboxyl esterase with the SEQ ID NO:46. Each of the polypeptide variants has an amino acid sequence shortened by 47 amino acids at the N terminus, and the amino acid sequences have at least 70%, preferably 80%, in particular 100%, sequence identity, namely SEQ ID NO:1, to the amino acid sequence portion 48-540 of the SEQ ID NO:46. The invention also relates to isolated polynucleotides which code for the polypeptide variants, to a fusarium toxin-cleaving additive containing at least one polypeptide variant and optionally at least one auxiliary agent, to the use of the polypeptide variants or the additive, and to a method for hydrolytically cleaving at least one fusarium toxin.