公开(公告)号：US20190153510A1

公开(公告)日：2019-05-23

申请号：US16134254

申请日：2018-09-18

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim Allawi ,  Rebecca Oldham-Haltom ,  Zubin Gagrat ,  Michael Domanico ,  Graham Lidgard

IPC: C12Q1/686 ,  C12Q1/6851

Abstract: Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.

公开(公告)号：US11499179B2

公开(公告)日：2022-11-15

申请号：US16789276

申请日：2020-02-12

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Rebecca Oldham-Haltom ,  Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12Q1/68 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6818 ,  C12Q1/6886

Abstract: A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

公开(公告)号：US10106844B2

公开(公告)日：2018-10-23

申请号：US14939651

申请日：2015-11-12

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Hatim Allawi ,  Rebecca Oldham-Haltom ,  Zubin Gagrat ,  Michael Domanico ,  Graham Lidgard

IPC: C12Q1/68 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6851

Abstract: Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.

公开(公告)号：US20200248245A1

公开(公告)日：2020-08-06

申请号：US16789276

申请日：2020-02-12

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Rebecca Oldham-Haltom ,  Hongzhi Zou ,  Graham P. Lidgard ,  Michael J. Domanico ,  Hatim Allawi

IPC: C12Q1/6827 ,  C12Q1/6886 ,  C12Q1/6818 ,  C12Q1/686

Abstract: A cleavage-based real-time PCR assay method is provided. In general terms, the assay method includes subjecting a reaction mixture comprising a) PCR reagents for amplifying a nucleic acid target, and b) flap cleavage reagents for performing a flap cleavage assay on the amplified nucleic acid target to two sets of thermocycling conditions. No additional reagents are added to the reaction between said first and second sets of cycles and, in each cycle of the second set of cycles, cleavage of a flap probe is measured.

公开(公告)号：US20220333210A1

公开(公告)日：2022-10-20

申请号：US17739892

申请日：2022-05-09

Applicant: Exact Sciences Development Company, LLC

Inventor： Rebecca Oldham-Haltom ,  Hatim Allawi ,  Hongzhi Zou ,  Michael J. Domanico ,  Graham P. Lidgard

IPC: C12Q1/6886 ,  C12Q1/6858

Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

公开(公告)号：US11352674B2

公开(公告)日：2022-06-07

申请号：US16814798

申请日：2020-03-10

Applicant: Exact Sciences Development Company, LLC

Inventor： Rebecca Oldham-Haltom ,  Hatim Allawi ,  Hongzhi Zou ,  Michael J. Domanico ,  Graham P. Lidgard

IPC: C12Q1/68 ,  C12Q1/6886 ,  C12Q1/6858

Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

公开(公告)号：US20200270706A1

公开(公告)日：2020-08-27

申请号：US16814798

申请日：2020-03-10

Applicant: Exact Sciences Development Company, LLC

Inventor： Rebecca Oldham-Haltom ,  Hatim Allawi ,  Hongzhi Zou ,  Michael J. Domanico ,  Graham P. Lidgard

IPC: C12Q1/6886 ,  C12Q1/6858

Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

公开(公告)号：US10093987B2

公开(公告)日：2018-10-09

申请号：US15686065

申请日：2017-08-24

Applicant: EXACT SCIENCES DEVELOPMENT COMPANY, LLC

Inventor： Rebecca Oldham-Haltom ,  Hatim Allawi ,  Hongzhi Zou ,  Michael J. Domanico ,  Graham P. Lidgard

IPC: C12Q1/68 ,  C12Q1/6886 ,  C12Q1/6858

Abstract: Provided herein is reagent mixture comprising multiplexed amplification reagents and flap assay reagents for detecting, in a single reaction, mutant copies of the KRAS gene that contain any of the 34A, 34C, 34T, 35A, 35C, 35T or 38A point mutations. Methods that employ the reagent mix and kits for performing the same are also provided.

公开(公告)号：US20210189462A1

公开(公告)日：2021-06-24

申请号：US17174127

申请日：2021-02-11

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim Allawi ,  Rebecca Oldham-Haltom ,  Zubin Gagrat ,  Michael Domanico ,  Graham Lidgard

IPC: C12Q1/686 ,  C12Q1/6851

Abstract: Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.

公开(公告)号：US10954556B2

公开(公告)日：2021-03-23

申请号：US16134254

申请日：2018-09-18

Applicant: Exact Sciences Development Company, LLC

Inventor： Hatim Allawi ,  Rebecca Oldham-Haltom ,  Zubin Gagrat ,  Michael Domanico ,  Graham Lidgard

IPC: C12Q1/686 ,  C12Q1/6851

Abstract: Provided herein is technology relating to the amplification-based detection of nucleic acids and particularly, but not exclusively, to methods and compositions for minimizing variability in the activity between different samples or manufacturing lots of DNA polymerases, such as Taq DNA polymerase.