公开(公告)号：US20180265875A1

公开(公告)日：2018-09-20

申请号：US15307455

申请日：2015-03-04

Applicant: East China University of Science and Technology

Inventor： Jianhe Xu ,  Yujun Zhang ,  Gaowei Zheng ,  Jiang Pan

IPC: C12N15/52 ,  C12N1/16 ,  C12N15/81 ,  C12R1/72 ,  C12N9/02 ,  C12P7/42

CPC classification number: C12N15/52 ,  C12N1/16 ,  C12N9/0036 ,  C12N15/81 ,  C12P7/42 ,  C12R1/72 ,  C12Y101/01184

Abstract: Disclosed herein is Candida parapsilosis CGMCC 9630, the carbonyl reductase expressed by said strain and the encoding gene and amino acid sequence thereof, the recombinant expression vector and recombinant expression transformant containing said gene sequence, and use of whole cells of Candida parapsilosis, carbonyl reductase or corresponding recombinant transformant thereof as catalyst in catalyzing asymmetric reduction of prochiral carbonyl compounds, particularly reduction of 6-carbonyl-8-halogenocaprylate to prepare the synthetic precursor of (R)-α-lipoic acid, (R)-6-hydroxy-8-halogenocaprylate. In comparison to other methods of asymmetric reduction for preparing (R)-6-hydroxy-8-halogenocaprylate, the disclosure has advantages of high substrate concentration, mild reaction conditions, environmental friendship, high yield, and high optical purity of the product, and thus has good prospect in industrial production of (R)-α-α-lipoic acid.

公开(公告)号：US10294479B2

公开(公告)日：2019-05-21

申请号：US15307455

申请日：2015-03-04

Applicant: East China University of Science and Technology

Inventor： Jianhe Xu ,  Yujun Zhang ,  Gaowei Zheng ,  Jiang Pan

IPC: C12N9/04 ,  C12N9/88 ,  C12N9/10 ,  C12N15/00 ,  C12P7/62 ,  C12P7/04 ,  C12N15/52 ,  C12R1/72 ,  C12N1/16 ,  C12P7/42 ,  C12N9/02 ,  C12N15/81

Abstract: Disclosed herein is Candida parapsilosis CGMCC 9630, the carbonyl reductase expressed by said strain and the encoding gene and amino acid sequence thereof, the recombinant expression vector and recombinant expression transformant containing said gene sequence, and use of whole cells of Candida parapsilosis, carbonyl reductase or corresponding recombinant transformant thereof as catalyst in catalyzing asymmetric reduction of prochiral carbonyl compounds, particularly reduction of 6-carbonyl-8-halogenocaprylate to prepare the synthetic precursor of (R)-α-lipoic acid, (R)-6-hydroxy-8-halogenocaprylate. In comparison to other methods of asymmetric reduction for preparing (R)-6-hydroxy-8-halogenocaprylate, the disclosure has advantages of high substrate concentration, mild reaction conditions, environmental friendship, high yield, and high optical purity of the product, and thus has good prospect in industrial production of (R)-α-α-lipoic acid.