公开(公告)号：US5589613A

公开(公告)日：1996-12-31

申请号：US150528

申请日：1993-11-10

申请人： Ebrahim Firoozabady ,  Christine Lemieux ,  Benjamin A. Moll ,  Karol Robinson

发明人： Ebrahim Firoozabady ,  Christine Lemieux ,  Benjamin A. Moll ,  Karol Robinson

IPC分类号： A01H4/00 ,  A01H5/02 ,  C12N15/82

CPC分类号： C12N15/8205 ,  A01H4/00 ,  A01H5/0233

摘要： Carnation plant material is transformed by cocultivation with Agrobacterium cells carrying an exogenous DNA sequence. In particular, by initiating callus formation on the plant material, transformed shoots may be produced in a suitable medium. Plantlets may be produced from the shoots by initiating root formation and subsequently transferring the rooted shoots to soil.

摘要翻译： 康乃馨植物材料通过与携带外源DNA序列的农杆菌细胞共培养而转化。 特别地，通过在植物材料上引发愈伤组织形成，可以在合适的培养基中产生转化芽。 可以通过引发根形成并随后将根茎转移到土壤中从枝条产生植物。

公开(公告)号：US4650336A

公开(公告)日：1987-03-17

申请号：US778497

申请日：1985-09-20

申请人： Benjamin A. Moll

发明人： Benjamin A. Moll

IPC分类号： G01N21/63 ,  G01N21/64 ,  G01N21/84 ,  G01J3/50

CPC分类号： G01N21/64 ,  G01N2021/635 ,  G01N2021/6484 ,  G01N2021/8466

摘要： A method of testing a plant to determine the variable fluorescence of the plant, and instrumentation for carrying out the method, is described. The method has use in determining the extent to which a plant has been damaged by photoinhibitory stress, or is susceptible to damage by such stress.

摘要翻译： 描述了测试植物以确定植物的可变荧光的方法以及用于实施该方法的仪器。 该方法用于确定植物受光抑制胁迫损伤的程度，或易受这种胁迫的损害。

公开(公告)号：US5270175A

公开(公告)日：1993-12-14

申请号：US729513

申请日：1991-07-12

申请人： Benjamin A. Moll

发明人： Benjamin A. Moll

IPC分类号： A01H13/00 ,  C12N9/04 ,  C12N9/88 ,  C12N15/79 ,  C12P7/06 ,  C12N5/14 ,  C12N15/82

CPC分类号： C12N9/0006 ,  A01H13/00 ,  C12N15/79 ,  C12N9/88 ,  C12P7/065 ,  Y02E50/17 ,  Y10S435/946

摘要： Soluble metabolic products, such as ethanol, are produced by growing modified algal cells in a growth medium and recovering the products from the growth medium. The algal cells are modified to overproduce the metabolic product by providing for overexpression of at least one enzyme in the metabolic pathway for the product. For the production of ethanol, the alcohol dehydrogenase gene, the pyruvate decarboxylase gene, or both, are overexpressed, typically under the control of a heterologous promoter. The algal cells may be modified by transformation with a DNA construct including coding sequence(s) of the enzyme(s) under the control of a heterologous promoter.

摘要翻译： 通过在生长培养基中生长修饰的藻类细胞并从生长培养基中回收产物来产生可溶性代谢产物，例如乙醇。 通过在产物的代谢途径中提供至少一种酶的过度表达，藻类细胞被修饰以过度产生代谢产物。 为了生产乙醇，醇脱氢酶基因，丙酮酸脱羧酶基因或两者都被过表达，通常在异源启动子的控制下。 藻类细胞可以通过用包含在异源启动子控制下的酶的编码序列的DNA构建体进行转化来修饰。