利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

25 条记录 (耗时 0.028 秒)

    Method for identifying regulators of protein-advanced glycation end product (protein-AGE) formation
    4.
    发明授权
    Method for identifying regulators of protein-advanced glycation end product (protein-AGE) formation 有权
    鉴定蛋白质晚期糖基化终产物（蛋白质 - AGE）形成的调节剂的方法

    公开(公告)号：US06716635B2

    公开(公告)日：2004-04-06

    申请号：US09836576

    申请日：2001-04-16

    申请人： Elaine L. Jacobson ,  Myron K. Jacobson ,  Georg T. Wondrak

    发明人： Elaine L. Jacobson ,  Myron K. Jacobson ,  Georg T. Wondrak

    IPC分类号： G01N3300

    CPC分类号： G01N33/6875 ,  G01N2400/00

    摘要： The invention relates to methods for identifying compounds which affect cellular stress. In particular, the method relates to identifying compounds which inhibit protein advanced glycation end product formation, where the compounds are carbonyl scavengers which inhibit the formation. The assay involves combing the substance of interest with histone H1 and ADP-ribose, and then measuring fluorescence and protein cross linking. Various inhibitors of protein AGE glycation have been identified, using this assay.

    摘要翻译： 本发明涉及鉴定影响细胞应激的化合物的方法。 特别地，该方法涉及鉴定抑制蛋白质晚期糖基化终产物形成的化合物，其中化合物是抑制形成的羰基清除剂。 该测定涉及将组合物H1和ADP-核糖组合感兴趣的物质，然后测量荧光和蛋白质交联。 使用该测定法已经鉴定了蛋白质AGE糖基化的各种抑制剂。

    Method for inducing cell death with carbonyl scavengers
    5.
    发明授权
    Method for inducing cell death with carbonyl scavengers 有权
    用羰基清除剂诱导细胞死亡的方法

    公开(公告)号：US07217739B2

    公开(公告)日：2007-05-15

    申请号：US11069292

    申请日：2005-03-01

    申请人： Georg T. Wondrak ,  Myron K. Jacobson ,  Elaine L. Jacobson

    发明人： Georg T. Wondrak ,  Myron K. Jacobson ,  Elaine L. Jacobson

    IPC分类号： A61K31/13 ,  A61K31/195

    CPC分类号： A61K31/155 ,  A61K31/195 ,  A61K31/198

    摘要： Carbonyl scavengers, which have been recognized for their ability to react with reactive carbonyl species, have been implicated as agents that can accelerate or induce the death of abnormal cells without having a parallel effect on normal cells. As such, they are useful therapeutic agents in cases where the acceleration or induction of cell death is desirable, such as in cells where the normal apoptotic pathways have been disrupted and where hyperproliferative cell growth occurs.

    摘要翻译： 被认为与反应性羰基物质反应的能力的羰基清除剂已经被认为是可以加速或诱导异常细胞死亡但不对正常细胞具有平行作用的试剂。 因此，在需要加速或诱导细胞死亡的情况下，例如在正常凋亡途径被破坏并且发生过度增殖细胞生长的细胞中，它们是有用的治疗剂。

    Genes encoding several poly (ADP-ribose) glycohydrolase (PARG) enzymes, the proteins and fragments thereof, and antibodies immunoreactive therewith
    6.
    发明授权
    Genes encoding several poly (ADP-ribose) glycohydrolase (PARG) enzymes, the proteins and fragments thereof, and antibodies immunoreactive therewith 有权
    编码几种聚（ADP-核糖）糖水解酶（PARG）酶的基因，其蛋白质及其片段和与其免疫反应的抗体

    公开(公告)号：US06333148B1

    公开(公告)日：2001-12-25

    申请号：US09302812

    申请日：1999-04-30

    申请人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

    发明人： Myron K. Jacobson ,  Elaine L. Jacobson ,  Jean-Christophe Amé ,  Winston Lin

    IPC分类号： C12Q168

    CPC分类号： C12Y302/01143 ,  A01K2217/075 ,  C07K16/40 ,  C07K2319/00 ,  C12N9/2402 ,  C12Q1/34

    摘要： The isolation and characterization of cDNAs encoding poly(ADP-ribose) glycohydrolase (PARG) enzymes and the amino acid sequences of PARGs from several species are described. PARG is involved in the cellular response to DNA damage and its proper function is associated with the body's response to neoplastic disorder inducing agents and oxidative stress. Expression vectors containing the cDNAs and cells transformed with the vectors are described. Probes and primers that hybridize with the cDNAs are described. Expression of the cDNA in E. coli results in an enzymatically active protein of about 111 kDa and an active fragment of about 59 kDa. Methods for inhibiting PARG expression or overexpressing PARG in a subject for therapeutic benefit are described. Exemplary of PARG inhibitors are anti-sense oligonucleotides. The invention has implications for treatment of neoplastic disorder, heart attack, stroke, and neurodegenerative diseases. Methods for detecting a mutant PARG allele are also described. Antibodies immunoreactive with PARGs and fragments thereof are described.

    摘要翻译： 描述了编码聚（ADP-核糖）糖水解酶（PARG）酶的cDNA和来自几种物种的PARG的氨基酸序列的分离和表征。 PARG参与对DNA损伤的细胞反应，其适当的功能与身体对肿瘤性疾病诱导剂和氧化应激的反应有关。 描述含有cDNA和用载体转化的细胞的表达载体。 描述了与cDNA杂交的探针和引物。 cDNA在大肠杆菌中的表达产生约111kDa的酶活性蛋白质和约59kDa的活性片段。 描述了用于抑制受试者中PARG表达或过表达PARG的治疗益处的方法。 PARG抑制剂的示例是反义寡核苷酸。 本发明对于治疗肿瘤性疾病，心脏病发作，中风和神经变性疾病具有意义。 还描述了用于检测突变型PARG等位基因的方法。 描述了与PARG及其片段免疫反应的抗体。

    Biochemical method to measure niacin status in a biological sample
    10.
    发明授权
    Biochemical method to measure niacin status in a biological sample 有权
    生物化学方法测定生物样品中的烟酸状态

    公开(公告)号：US06428972B2

    公开(公告)日：2002-08-06

    申请号：US09871259

    申请日：2001-05-31

    申请人： Elaine L. Jacobson ,  Myron K. Jacobson

    发明人： Elaine L. Jacobson ,  Myron K. Jacobson

    IPC分类号： C12Q132

    CPC分类号： C12Q1/32 ,  G01N33/82

    摘要： The invention relates to assay or determining intercellular niacin status in solid tissues. One extracts intracellular pyredine nucleotides, cycles these, in the presence of agents which reduce the pyredine nucleotides and a dye, which reflect the change. Changes in absorbance of the dye molecule permit determination of the niacin status.

    摘要翻译： 本发明涉及测定或确定固体组织中的细胞间烟酸状态。 一个提取细胞内pyredine核苷酸，循环这些，在减少pyredine核苷酸的试剂和一种反映变化的染料的存在下。 染料分子的吸光度变化允许测定烟酸状态。