Heterologous biomass degrading enzyme expression in thermoanaerobacterium saccharolyticum
    2.
    发明授权
    Heterologous biomass degrading enzyme expression in thermoanaerobacterium saccharolyticum 有权
    异烟生物降解酶在酵母菌中的表达

    公开(公告)号:US09206434B2

    公开(公告)日:2015-12-08

    申请号:US13141952

    申请日:2009-12-23

    摘要: Thermophilic gram-positive anaerobic host cells, for example Thermoanaerobacterium saccharolyticum (“T sacch”), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose. Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO2. In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide. In addition, the host cell, for example, a T sacch host cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol.

    摘要翻译: 嗜热革兰氏阳性厌氧宿主细胞,例如解糖热酵母杆菌(“T sacch”)表达异源生物降解酶,如纤维素酶,并且能够从纤维素产生有用的发酵产物。 有用的发酵产物包括例如乙醇,乙酸,乳酸或二氧化碳。 为了提供最大的表达和活性水平,生物降解酶可以由密码子优化的核苷酸序列表达,可以在高效启动子的控制下表达,和/或可以与信号肽融合。 此外,宿主细胞,例如T细胞宿主细胞,可进行遗传改变以进一步改善乙醇生产,例如通过破坏除乙醇以外的有机产物的生产。

    Heterologous expression of termite cellulases yeast
    4.
    发明授权
    Heterologous expression of termite cellulases yeast 有权
    白蚁纤维素酶酵母的异源表达

    公开(公告)号:US08658398B2

    公开(公告)日:2014-02-25

    申请号:US13003200

    申请日:2009-07-07

    IPC分类号: C12P19/14

    摘要: The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

    摘要翻译: 本发明提供白蚁和白蚁相关共生体纤维素酶的异源表达。 纤维素酶可以例如密码子优化并在酵母宿主细胞中表达,例如酵母酿酒酵母(Saccharomyces cerevisiae)。 纤维素酶也可以与其他纤维素酶在宿主细胞中共表达。 在白蚁和白蚁相关共生体纤维素酶的这种宿主细胞中的表达及其变体和组合导致具有改善的纤维素活性的酵母。 因此,这些基因和表达系统对于有效和成本有效的整合生物处理系统是有用的。

    Heterologous Biomass Degrading Enzyme Expression in Thermoanaerobacterium Saccharolyticum
    7.
    发明申请
    Heterologous Biomass Degrading Enzyme Expression in Thermoanaerobacterium Saccharolyticum 有权
    异源生物量降解酵母菌中的酶表达

    公开(公告)号:US20120040409A1

    公开(公告)日:2012-02-16

    申请号:US13141952

    申请日:2009-12-23

    摘要: Thermophilic gram-positive anaerobic host cells, for example Thermoanaerobacterium saccharolyticum (“T sacch”), express heterologous biomass degrading enzymes, such as cellulases, and are able to produce useful fermentation products from cellulose. Useful fermentation products include, for example, ethanol, acetic acid, lactic acid or CO2. In order to provide maximum expression and activity levels, biomass degrading enzymes can be expressed from codon-optimized nucleotide sequences, can be expressed under the control of a high-efficiency promoter, and/or can be fused to a signal peptide. In addition, the host cell, for example, a T sacch host cell, can be genetically altered to further improve ethanol production, for example by disrupting the production of organic products other than ethanol.

    摘要翻译: 嗜热革兰氏阳性厌氧宿主细胞,例如解糖热酵母杆菌(“T sacch”)表达异源生物降解酶,如纤维素酶,并且能够从纤维素产生有用的发酵产物。 有用的发酵产物包括例如乙醇,乙酸,乳酸或二氧化碳。 为了提供最大的表达和活性水平,生物降解酶可以由密码子优化的核苷酸序列表达,可以在高效启动子的控制下表达,和/或可以与信号肽融合。 此外,宿主细胞,例如T细胞宿主细胞,可进行遗传改变以进一步改善乙醇生产,例如通过破坏除乙醇以外的有机产物的生产。