公开(公告)号：US20130323822A1

公开(公告)日：2013-12-05

申请号：US13701652

申请日：2011-06-03

申请人： Elena Brevnova ,  John E. McBride ,  Erin Wiswall ,  Kevin S. Wenger ,  Nicky Caiazza ,  Heidi Hau ,  Aaron Argyros ,  Frank Agbogbo ,  Charles F. Rice ,  Trisha Barrett ,  John S. Bardsley ,  Abigail S. Foster ,  Anne K. Warner ,  Mark Mellon ,  Ryan Skinner ,  Indraneel Shikhare ,  Riaan Den Haan ,  Chhayal V. Gandhi ,  Alan Belcher ,  Vineet B. Rajgarhia ,  Allan C. Froehlich ,  Kristen M. Deleault ,  Emily Stonehouse ,  Shital A. Tripathi ,  Jennifer Gosselin ,  Yin-Ying Chiu ,  Haowen Xu

发明人： Elena Brevnova ,  John E. McBride ,  Erin Wiswall ,  Kevin S. Wenger ,  Nicky Caiazza ,  Heidi Hau ,  Aaron Argyros ,  Frank Agbogbo ,  Charles F. Rice ,  Trisha Barrett ,  John S. Bardsley ,  Abigail S. Foster ,  Anne K. Warner ,  Mark Mellon ,  Ryan Skinner ,  Indraneel Shikhare ,  Riaan Den Haan ,  Chhayal V. Gandhi ,  Alan Belcher ,  Vineet B. Rajgarhia ,  Allan C. Froehlich ,  Kristen M. Deleault ,  Emily Stonehouse ,  Shital A. Tripathi ,  Jennifer Gosselin ,  Yin-Ying Chiu ,  Haowen Xu

IPC分类号： C12N15/81

CPC分类号： C12N15/81 ,  C12N9/2405 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N9/2482 ,  C12N15/52 ,  C12P7/06 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

摘要翻译： 本发明涉及分泌全套或全套的酶的酵母菌株或菌株，以水解玉米淀粉，玉米纤维，木质纤维素（包括水解纤维素中的连接酶，半纤维素和 木质素和碳水化合物之间）并利用戊糖（木糖和阿拉伯糖）。 本发明还涉及在酵母中对于各种酶活性良好表达的蛋白质组。 所得菌株或菌株可以同时水解淀粉和纤维素。 所产生的菌株或菌株也可以代谢工程化以产生较少的甘油和吸收乙酸盐。 所得的菌株或菌株也可用于从颗粒淀粉生产乙醇而不液化。 所得到的菌株或菌株可以进一步用于减少在同时糖化和发酵（SSF）过程中水解生物质原料所需的外部酶的量，或者在当前蔗糖分解酶负载下在SSF期间提高乙醇的产率。 此外，本发明的多种酶可以在本发明的细胞中共表达以提供对生物质原料的协同消化作用。 在一些方面，表达不同异源糖分解酶的宿主细胞也可以共同共培养并用于从生物质原料生产乙醇。

公开(公告)号：US09957530B2

公开(公告)日：2018-05-01

申请号：US13391554

申请日：2010-08-20

申请人： John E. McBride ,  Vineet Rajgarhia ,  Arthur J. Shaw, IV ,  Shital A. Tripathi ,  Elena Brevnova ,  Nicky Caiazza ,  Johannes Pieter Van Dijken ,  Allan C. Froehlich ,  William Ryan Sillers ,  James H. Flatt

发明人： John E. McBride ,  Vineet Rajgarhia ,  Arthur J. Shaw, IV ,  Shital A. Tripathi ,  Elena Brevnova ,  Nicky Caiazza ,  Johannes Pieter Van Dijken ,  Allan C. Froehlich ,  William Ryan Sillers ,  James H. Flatt

IPC分类号： C12N1/00 ,  C12N1/20 ,  C12P7/04 ,  C12P7/18 ,  C12Q1/00 ,  C12Q1/68 ,  C07H21/04 ,  C12P7/36

CPC分类号： C12P7/18 ,  C12P7/04 ,  C12P7/36 ,  C12P2203/00

摘要： The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, upregulated or downregulated.

公开(公告)号：US20120322078A1

公开(公告)日：2012-12-20

申请号：US13391554

申请日：2010-08-20

申请人： John E. Mcbride ,  Vineet Rajgarhia ,  Arthur J. Shaw ,  Shital A. Tripathi ,  Elena Brevnova ,  Nicky Caiazza ,  Johannes Pieter Van Dijken ,  Allan C. Froehlich ,  William Ryan Sillers ,  James H. Flatt

发明人： John E. Mcbride ,  Vineet Rajgarhia ,  Arthur J. Shaw ,  Shital A. Tripathi ,  Elena Brevnova ,  Nicky Caiazza ,  Johannes Pieter Van Dijken ,  Allan C. Froehlich ,  William Ryan Sillers ,  James H. Flatt

IPC分类号： C12P7/04 ,  C12P7/18 ,  C12Q1/68 ,  C12Q1/527 ,  C12N1/19 ,  C12N1/21 ,  C12N15/60

CPC分类号： C12P7/18 ,  C12P7/04 ,  C12P7/36 ,  C12P2203/00

摘要： The present invention provides for novel metabolic pathways leading to propanol, alcohol or polyol formation in a consolidated bioprocessing system (CBP), where lignocellulosic biomass is efficiently converted to such products. More specifically, the invention provides for a recombinant microorganism, where the microorganism expresses one or more native and/or heterologous enzymes; where the one or more enzymes function in one or more engineered metabolic pathways to achieve: (1) conversion of a carbohydrate source to 1,2-propanediol, isopropropanol, ethanol and/or glycerol; (2) conversion of a carbohydrate source to n-propanol and isopropanol; (3) conversion of a carbohydrate source to isopropanol and methanol; or (4) conversion of a carbohydrate source to propanediol and acetone; wherein the one or more native and/or heterologous enzymes is activated, up-regulated or down-regulated.

摘要翻译： 本发明提供了在固结生物处理系统（CBP）中导致丙醇，醇或多元醇形成的新型代谢途径，其中木质纤维素生物质被有效地转化成这样的产物。 更具体地，本发明提供重组微生物，其中微生物表达一种或多种天然和/或异源酶; 其中一种或多种酶在一个或多个工程化代谢途径中起作用以实现：（1）将碳水化合物源转化为1,2-丙二醇，异丙醇，乙醇和/或甘油; （2）将碳水化合物源转化为正丙醇和异丙醇; （3）将碳水化合物源转化成异丙醇和甲醇; 或（4）将碳水化合物源转化为丙二醇和丙酮; 其中所述一种或多种天然和/或异源酶被激活，上调或下调。

公开(公告)号：US20130273555A1

公开(公告)日：2013-10-17

申请号：US13696207

申请日：2011-05-05

申请人： William Ryan Sillers ,  Hans Van Dijken ,  Steve Licht ,  Arthur J. Shaw, IV ,  Alan Benjamin Gilbert ,  Aaron Argyros ,  Allan C. Froehlich ,  John E. McBride ,  Haowen Xu ,  David A. Hogsett ,  Vineet B. Rajgarhia

发明人： William Ryan Sillers ,  Hans Van Dijken ,  Steve Licht ,  Arthur J. Shaw, IV ,  Alan Benjamin Gilbert ,  Aaron Argyros ,  Allan C. Froehlich ,  John E. McBride ,  Haowen Xu ,  David A. Hogsett ,  Vineet B. Rajgarhia

IPC分类号： C12N15/81 ,  C12N15/74 ,  C12R1/865 ,  C12P7/04 ,  C12P7/62 ,  C12N1/16 ,  C12P7/10 ,  C12P7/28

CPC分类号： C12N15/81 ,  C12N1/16 ,  C12N1/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0067 ,  C12N15/74 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/10 ,  C12P7/28 ,  C12P7/62 ,  C12R1/01 ,  C12R1/645 ,  C12R1/865 ,  C12Y102/01012 ,  C12Y112/99006 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/52

摘要： One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce lactate or acetate as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of lactate or acetate. Another aspect of the invention relates to a process for converting lignocellulosic biomass to lactate or acetate, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.

摘要翻译： 本发明的一个方面涉及经遗传修饰的嗜热或嗜温微生物，其中第一天然基因部分，基本上或完全缺失，沉默，失活或下调，所述第一天然基因编码参与 代谢产生有机酸或其盐，从而增加所述嗜热或嗜温微生物产生作为发酵产物的乳酸盐或乙酸盐的天然能力。 在某些实施方案中，上述微生物还包含第一非天然基因，其第一非天然基因编码参与乳酸盐或乙酸盐的代谢产生的第一非天然酶。 本发明的另一方面涉及将木质纤维素生物质转化为乳酸盐或乙酸盐的方法，包括使木质纤维素生物质与遗传修饰的嗜热或嗜温微生物接触。

公开(公告)号：US09605269B2

公开(公告)日：2017-03-28

申请号：US13696207

申请日：2011-05-05

申请人： William Ryan Sillers ,  Hans Van Dijken ,  Steve Licht ,  Arthur J. Shaw, IV ,  Alan Benjamin Gilbert ,  Aaron Argyros ,  Allan C. Froehlich ,  John E. McBride ,  Haowen Xu ,  David A. Hogsett ,  Vineet B. Rajgarhia

发明人： William Ryan Sillers ,  Hans Van Dijken ,  Steve Licht ,  Arthur J. Shaw, IV ,  Alan Benjamin Gilbert ,  Aaron Argyros ,  Allan C. Froehlich ,  John E. McBride ,  Haowen Xu ,  David A. Hogsett ,  Vineet B. Rajgarhia

IPC分类号： C12N1/20 ,  C12N15/81 ,  C12N1/18 ,  C12N9/04 ,  C12N9/02 ,  C12P7/02 ,  C12P7/06 ,  C12P7/10 ,  C12P7/28 ,  C12P7/62 ,  C12R1/01 ,  C12R1/645 ,  C12N1/16 ,  C12N15/74 ,  C12P7/04 ,  C12R1/865

CPC分类号： C12N15/81 ,  C12N1/16 ,  C12N1/18 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0067 ,  C12N15/74 ,  C12P7/02 ,  C12P7/04 ,  C12P7/06 ,  C12P7/10 ,  C12P7/28 ,  C12P7/62 ,  C12R1/01 ,  C12R1/645 ,  C12R1/865 ,  C12Y102/01012 ,  C12Y112/99006 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/52

摘要： One aspect of the invention relates to a genetically modified thermophilic or mesophilic microorganism, wherein a first native gene is partially, substantially, or completely deleted, silenced, inactivated, or down-regulated, which first native gene encodes a first native enzyme involved in the metabolic production of an organic acid or a salt thereof, thereby increasing the native ability of said thermophilic or mesophilic microorganism to produce lactate or acetate as a fermentation product. In certain embodiments, the aforementioned microorganism further comprises a first non-native gene, which first non-native gene encodes a first non-native enzyme involved in the metabolic production of lactate or acetate. Another aspect of the invention relates to a process for converting lignocellulosic biomass to lactate or acetate, comprising contacting lignocellulosic biomass with a genetically modified thermophilic or mesophilic microorganism.

公开(公告)号：US06733996B2

公开(公告)日：2004-05-11

申请号：US10228876

申请日：2002-08-26

申请人： Allan C. Froehlich ,  Jennifer Loros ,  Jay C. Dunlap

发明人： Allan C. Froehlich ,  Jennifer Loros ,  Jay C. Dunlap

IPC分类号： C12P2102

CPC分类号： C12N15/79 ,  C07H21/04 ,  C12N15/635

摘要： The invention provides methods of regulating gene expression using a light-activated transcription factor. The White Collar Complex, comprised of WC-1 and WC-2 proteins, binds FAD and becomes activated in the presence of light to stimulate transcription of genes operatively-linked to light-responsive regulatory sequences. Regulatory sequences and inducible regulatory system kits are also provided.

摘要翻译： 本发明提供使用光激活转录因子调节基因表达的方法。 由WC-1和WC-2蛋白组成的白领复合物与FAD结合并在光的存在下被激活以刺激与光响应性调控序列有效连接的基因的转录。 还提供了调节序列和诱导型调节系统试剂盒。