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    Genetically modified clostridium thermocellum engineered to ferment xylose
    4.
    发明授权
    Genetically modified clostridium thermocellum engineered to ferment xylose 有权
    转基因热纤维素梭菌工程发酵木糖

    公开(公告)号：US09546385B2

    公开(公告)日：2017-01-17

    申请号：US13995402

    申请日：2011-12-22

    申请人： Aaron Argyros ,  Trisha Barrett ,  Nicky Caiazza ,  Dave Hogsett

    发明人： Aaron Argyros ,  Trisha Barrett ,  Nicky Caiazza ,  Dave Hogsett

    IPC分类号： C12P7/06 ,  C12N9/12 ,  C12N15/74 ,  C12N1/20 ,  C12N9/92 ,  C12P7/54 ,  C12P7/10

    CPC分类号： C12P7/06 ,  C12N1/20 ,  C12N9/1205 ,  C12N9/92 ,  C12N15/74 ,  C12P7/065 ,  C12P7/10 ,  C12P7/54 ,  C12Y207/01017 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/52

    摘要： One aspect of the invention relates to industrial bioconversion of the xylose portion of biomass materials into fuels and chemicals. Another aspect of the invention relates to industrial bioconversion of the xylan portion of biomass materials into fuels and chemicals. In one embodiment, the invention is directed to the bacterium Clostridium thermocellum, a highly cellulolytic organism that has much potential as a biocatalyst in a consolidated bioprocess configuration. In some embodiments, the invention is a genetic modification that confers the ability to ferment xylose to C. thermocellum and the strains created with this modification. In some embodiments, the genetic modification is composed of two genes contained in an operon from T. saccharolyticum. The genes express proteins with xylose isomerase (XI) and xylulokinase activites (XK). In other embodiments, the invention relates to a recombinant Clostridium thermocellum host cell capable of fermenting xylan.

    摘要翻译： 本发明的一个方面涉及生物质材料的木糖部分的工业生物转化为燃料和化学品。 本发明的另一方面涉及将生物质材料的木聚糖部分转化为燃料和化学品的工业生物转化。 在一个实施方案中，本发明涉及细菌嗜热纤维梭菌（Clostridium thermocellum），一种高度纤维素分解的生物体，其在整合的生物过程构型中作为生物催化剂具有很大的潜力。 在一些实施方案中，本发明是赋予将木糖发酵至热纤维梭菌的能力的遗传修饰以及用该修饰产生的菌株。 在一些实施方案中，遗传修饰由来自解糖松解糖酵母的操纵子中包含的两个基因组成。 该基因表达具有木糖异构酶（XI）和木酮糖激酶活性（XK）的蛋白质。 在其他实施方案中，本发明涉及能够发酵木聚糖的重组热纤梭菌宿主细胞。

    GENETICALLY MODIFIED CLOSTRIDIUM THERMOCELLUM ENGINEERED TO FERMENT XYLOSE
    5.
    发明申请
    GENETICALLY MODIFIED CLOSTRIDIUM THERMOCELLUM ENGINEERED TO FERMENT XYLOSE 有权
    工程化学发光二极管的遗传修饰的离体热电偶

    公开(公告)号：US20140370561A1

    公开(公告)日：2014-12-18

    申请号：US13995402

    申请日：2011-12-22

    申请人： Aaron Argyros ,  Trisha Barrett ,  Nicky Caiazza ,  Dave Hogsett

    发明人： Aaron Argyros ,  Trisha Barrett ,  Nicky Caiazza ,  Dave Hogsett

    IPC分类号： C12P7/06 ,  C12N9/12 ,  C12N15/74

    CPC分类号： C12P7/06 ,  C12N1/20 ,  C12N9/1205 ,  C12N9/92 ,  C12N15/74 ,  C12P7/065 ,  C12P7/10 ,  C12P7/54 ,  C12Y207/01017 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/52

    摘要： One aspect of the invention relates to industrial bioconversion of the xylose portion of biomass materials into fuels and chemicals. Another aspect of the invention relates to industrial bioconversion of the xylan portion of biomass materials into fuels and chemicals. In one embodiment, the invention is directed to the bacterium Clostridium thermocellum, a highly cellulolytic organism that has much potential as a biocatalyst in a consolidated bioprocess configuration. In some embodiments, the invention is a genetic modification that confers the ability to ferment xylose to C. thermocellum and the strains created with this modification. In some embodiments, the genetic modification is composed of two genes contained in an operon from T. saccharolyticum. The genes express proteins with xylose isomerase (XI) and xylulokinase activites (XK). In other embodiments, the invention relates to a recombinant Clostridium thermocellum host cell capable of fermenting xylan.

    摘要翻译： 本发明的一个方面涉及生物质材料的木糖部分的工业生物转化为燃料和化学品。 本发明的另一方面涉及将生物质材料的木聚糖部分转化为燃料和化学品的工业生物转化。 在一个实施方案中，本发明涉及细菌嗜热纤维梭菌（Clostridium thermocellum），一种高度纤维素分解的生物体，其在整合的生物过程构型中作为生物催化剂具有很大的潜力。 在一些实施方案中，本发明是赋予将木糖发酵至热纤维梭菌的能力的遗传修饰以及用该修饰产生的菌株。 在一些实施方案中，遗传修饰由来自解糖松解糖酵母的操纵子中包含的两个基因组成。 该基因表达具有木糖异构酶（XI）和木酮糖激酶活性（XK）的蛋白质。 在其他实施方案中，本发明涉及能够发酵木聚糖的重组热纤梭菌宿主细胞。

    POSITIVE AND NEGATIVE SELECTABLE MARKERS FOR USE IN THERMOPHILIC ORGANISMS
    6.
    发明申请
    POSITIVE AND NEGATIVE SELECTABLE MARKERS FOR USE IN THERMOPHILIC ORGANISMS 审中-公开
    积极和负面的可选标记用于热化学有机体

    公开(公告)号：US20130052646A1

    公开(公告)日：2013-02-28

    申请号：US13393093

    申请日：2010-08-10

    申请人： Shital Tripathi ,  Aaron Argyros ,  Trisha Barrett ,  Nicky Caiazza ,  Bethany B. Miller ,  Arthur J. Shaw, IV

    发明人： Shital Tripathi ,  Aaron Argyros ,  Trisha Barrett ,  Nicky Caiazza ,  Bethany B. Miller ,  Arthur J. Shaw, IV

    IPC分类号： C12N15/74 ,  C12Q1/68 ,  C12N1/21

    CPC分类号： C12N15/74

    摘要： The present invention relates to the field of molecular biology and genetic tool development in thermophilic bacteria. In particular, it relates to the use of positive and/or negative selection markers that can be used to efficiently select modified strains of interest. By providing such capabilities, the disclosed invention facilitates the recycling of genetic markers in thermophilic bacterial host cells. The present invention also allows the creation of unmarked strains. The genetic tools disclosed in the present invention are prerequisites for making targeted higher order mutations in a single thermophilic strain background.

    摘要翻译： 本发明涉及嗜热细菌的分子生物学和遗传工具开发领域。 特别地，本发明涉及使用可用于有效选择修饰的感兴趣菌株的阳性和/或阴性选择标记。 通过提供这样的能力，所公开的发明有利于在嗜热细菌宿主细胞中回收遗传标记。 本发明还允许产生未标记的菌株。 本发明公开的遗传工具是在单个嗜热菌株背景中进行靶向高级突变的先决条件。

    Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors
    7.
    发明授权
    Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors 有权
    通过添加交替的电子受体来提高微生物产物产率和生产的方法

    公开(公告)号：US08956851B2

    公开(公告)日：2015-02-17

    申请号：US14110075

    申请日：2012-04-05

    申请人： Aaron Argyros ,  William Ryan Sillers ,  Trisha Barrett ,  Nicky Caiazza ,  Arthur J. Shaw, IV

    发明人： Aaron Argyros ,  William Ryan Sillers ,  Trisha Barrett ,  Nicky Caiazza ,  Arthur J. Shaw, IV

    IPC分类号： C12N9/88 ,  C12N15/52 ,  C12N15/74 ,  C12N15/81 ,  C12N9/04 ,  C12N9/02 ,  C12P7/10 ,  C12P7/40

    CPC分类号： C12N15/81 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12P7/10 ,  C12P7/40 ,  C12Y102/0101 ,  C12Y203/01054 ,  Y02E50/16 ,  Y02E50/17

    摘要： The present invention provides for novel metabolic pathways to reduce or eliminate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising a deletion of one or more native enzymes that function to produce glycerol and/or regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous enzymes is activated, upregulated, or downregulated. The invention also provides for a recombinant microorganism comprising one or more heterologous enzymes that function to regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to ethanol, wherein said one or more native and/or heterologous enzymes is activated, upregulated or downregulated.

    摘要翻译： 本发明提供了用于减少或消除甘油产生并增加产物形成的新型代谢途径。 更具体地，本发明提供了重组微生物，其包含一种或多种功能于产生甘油和/或调节甘油合成的天然酶的缺失，以及在一个或多个工程化代谢途径中起作用的一种或多种天然和/或异源酶 将碳水化合物源（例如木质纤维素）转化成产物（例如乙醇），其中一种或多种天然和/或异源酶被活化，上调或下调。 本发明还提供了一种重组微生物，其包含一种或多种用于调节甘油合成的异源酶和一种或多种在一种或多种工程化代谢途径中起作用以将碳水化合物源转化为乙醇的天然和/或异源酶，其中所述一种 或更多的天然和/或异源酶被激活，上调或下调。

    Methods for the Improvement of Product Yield and Production in a Microorganism Through the Addition of Alternate Electron Acceptors
    8.
    发明申请
    Methods for the Improvement of Product Yield and Production in a Microorganism Through the Addition of Alternate Electron Acceptors 有权
    通过添加替代电子受体来提高产品产量和微生物生产的方法

    公开(公告)号：US20140186930A1

    公开(公告)日：2014-07-03

    申请号：US14110075

    申请日：2012-04-05

    申请人： Aaron Argyros ,  William Ryan Silllers ,  Trisha Barrett ,  Nicky Caiazza ,  Arthur J. Shaw, IV

    发明人： Aaron Argyros ,  William Ryan Silllers ,  Trisha Barrett ,  Nicky Caiazza ,  Arthur J. Shaw, IV

    IPC分类号： C12N15/81

    CPC分类号： C12N15/81 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12P7/10 ,  C12P7/40 ,  C12Y102/0101 ,  C12Y203/01054 ,  Y02E50/16 ,  Y02E50/17

    摘要： The present invention provides for novel metabolic pathways to reduce or eliminate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising a deletion of one or more native enzymes that function to produce glycerol and/or regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and/or heterologous enzymes is activated, upregulated, or downregulated. The invention also provides for a recombinant microorganism comprising one or more heterologous enzymes that function to regulate glycerol synthesis and one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to ethanol, wherein said one or more native and/or heterologous enzymes is activated, upregulated or downregulated.

    摘要翻译： 本发明提供了用于减少或消除甘油产生并增加产物形成的新型代谢途径。 更具体地，本发明提供了重组微生物，其包含一种或多种功能于产生甘油和/或调节甘油合成的天然酶的缺失，以及在一个或多个工程化代谢途径中起作用的一种或多种天然和/或异源酶 将碳水化合物源（例如木质纤维素）转化成产物（例如乙醇），其中一种或多种天然和/或异源酶被活化，上调或下调。 本发明还提供了一种重组微生物，其包含一种或多种用于调节甘油合成的异源酶和一种或多种在一种或多种工程化代谢途径中起作用以将碳水化合物源转化为乙醇的天然和/或异源酶，其中所述一种 或更多的天然和/或异源酶被激活，上调或下调。

    Production of Malonyl-CoA Derived Products Via Anaerobic Pathways
    9.
    发明申请
    Production of Malonyl-CoA Derived Products Via Anaerobic Pathways 审中-公开
    通过厌氧途径生产丙二酰辅酶A衍生产品

    公开(公告)号：US20130323766A1

    公开(公告)日：2013-12-05

    申请号：US13814616

    申请日：2011-08-05

    申请人： William Ryan Sillers ,  Shital A. Tripathi ,  Arthur J. Shaw ,  Aaron Argyros ,  David A. Hogsett

    发明人： William Ryan Sillers ,  Shital A. Tripathi ,  Arthur J. Shaw ,  Aaron Argyros ,  David A. Hogsett

    IPC分类号： C12P17/18

    CPC分类号： C12P17/182 ,  C12N1/22 ,  C12N9/1018 ,  C12N9/88 ,  C12P7/6409 ,  G01N2333/91034

    摘要： The present invention provides for novel metabolic pathways to convert biomass and other carbohydrate sources to malonyl-CoA derived products, such as hydrocarbons and other bioproducts, under anaerobic conditions and with the net production of ATP. More specifically, the invention provides for a recombinant microorganism comprising one or more native and/or heterologous enzymes that function in one or more engineered metabolic pathways to achieve conversion of a carbohydrate source to, e.g., long-chain hydrocarbons and hydrocarbon derivatives, wherein the one or more native and/or heterologous enzymes is activated, upregulated, downregulated, or deleted. The invention also provides for processes to convert biomass to malonyl-CoA derived products which comprise contacting a carbohydrate source with a recombinant microorganism of the invention.

    摘要翻译： 本发明提供了在厌氧条件下和ATP的净产生下将生物质和其他碳水化合物来源转化成丙二酰辅酶A衍生产物如烃和其他生物产物的新型代谢途径。 更具体地，本发明提供了一种重组微生物，其包含在一个或多个工程化代谢途径中起作用以实现碳水化合物源转化为例如长链烃和烃衍生物的一种或多种天然和/或异源酶，其中 一种或多种天然和/或异源酶被激活，上调，下调或缺失。 本发明还提供将生物质转化为丙二酰辅酶A衍生产物的方法，其包括使碳水化合物源与本发明的重组微生物接触。