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公开(公告)号：US11512304B2
公开(公告)日：2022-11-29
申请号：US17045088
申请日：2019-04-01
发明人： Haibin Chen , Yong Koy Bong , Qing Xu , Dongxiao Hu , Chengxiao Zhang , Baoqin Cai
摘要： Provided herein are engineered decarboxylase polypeptides that are useful for catalyzing the decarboxylation of amino acids such as L-tyrosine to produce tyramine or catalyzing the decarboxylation of L-DOPA to produce dopamine. Also provided are the preparation process of engineered decarboxylase polypeptides as well as reaction process under industrial-relevant conditions. The disclosure also provides polynucleotide sequences encoding engineered decarboxylase polypeptides, recombinant host cells capable of expressing engineered decarboxylase polypeptides, and methods of producing tyramine or dopamine using the engineered decarboxylase polypeptides. Compared to the wild type decarboxylase, the engineered polypeptide provided by this disclosure has better activity and/or stability. The use of the engineered polypeptides for the preparation of tyramine or dopamine reduces the production cost and has a good industrial application prospect.
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2.发明授权公开(公告)号：US11512303B2
公开(公告)日：2022-11-29
申请号：US16615120
申请日：2018-05-09
发明人： Haibin Chen , Yong Koy Bong , Qing Xu , Ameng Zhou , Tianran Shen , Jiadong Yang , Zhuhong Yang
摘要： The present invention provides engineered polypeptides that are useful for the asymmetric synthesis of β-hydroxy-α-amino acids under industrial-relevant conditions. The engineered polypeptides disclosed in this invention were developed through directed evolution based on the ability of catalytic synthesis of (2S, 3R)-2-amino-3-hydroxy-3-(4-nitrophenyl) propanoic acid. The present disclosure also provides polynucleotides encoding engineered polypeptides, host cells capable of expressing engineered polypeptides, and methods of producing β-hydroxy-α-amino acids using engineered polypeptides. Compared to other processes of preparation, the use of the engineered polypeptides of the present invention for the preparation of β-hydroxy-α-amino acids results in high purity of the desired stereoisomers, mild reaction conditions, low pollution and low energy consumption. So, it has good industrial application prospects.
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公开(公告)号：US11274287B2
公开(公告)日：2022-03-15
申请号：US16616370
申请日：2018-05-09
发明人： Haibin Chen , Yong Koy Bong , Baoqin Cai , Qing Xu , Tianran Shen , Ameng Zhou , Jiadong Yang , Zhuhong Yang , Yaoyao Ji , Yingxin Zhang
摘要： The present invention provides engineered polypeptides that are useful for the asymmetric synthesis of β-hydroxy-α-amino acids under industrial-relevant conditions. The present disclosure also provides polynucleotides encoding engineered polypeptides, host cells capable of expressing engineered polypeptides, and methods of producing β-hydroxy-α-amino acids using engineered polypeptides. Compared to other processes of preparation, the use of the engineered polypeptides of the present invention for the preparation of β-hydroxy-α-amino acids results in high purity of the desired stereoisomers, mild reaction conditions, low pollution and low energy consumption. So, it has good industrial application prospects.
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