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公开(公告)号：US07612250B2
公开(公告)日：2009-11-03
申请号：US10208653
申请日：2002-07-29
CPC分类号： C12N15/8771 , A01K2227/101 , C12N15/873
摘要： A nuclear transfer embryo is formed by destabilizing microtubules of an oocyte, whereby essentially all endogenous chromatin collects at a second polar body during meiosis of an oocyte. The oocyte is fused with the nucleus of a donor somatic cell of the same species of said oocyte prior to cessation of extrusion of the second polar body from the oocyte, thereby forming the nuclear transfer embryo. In one embodiment, the nuclear transfer embryo is employed to impregnate an animal, such as a mammal. In another embodiment, the donor nucleus is transgenic.
摘要翻译： 通过使卵母细胞的微管失稳来形成核转移胚，由此在卵母细胞减数分裂期间，基本上所有内源性染色质在第二极体收集。 在从卵母细胞停止挤出第二极体之前，卵母细胞与相同种类的所述卵母细胞的供体体细胞的细胞核融合，从而形成核转移胚。 在一个实施方案中，使用核转移胚来浸渍动物如哺乳动物。 在另一个实施方案中，供体核是转基因的。
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公开(公告)号：US20100205678A1
公开(公告)日：2010-08-12
申请号：US12448699
申请日：2008-01-04
IPC分类号： C12N15/06 , A01K67/027 , C12P21/00 , C12N5/07
CPC分类号： C12N15/8775 , A01K2217/05 , A01K2227/105 , A61D19/04 , C12N5/0609
摘要： The invention pertains to the discovery that the presence of oocyte spindle associated factors in an enucleated oocyte improves oocyte quality and subsequently nuclear transfer. In particular, it was discovered that maintaining oocyte spindle factors in the oocyte after enucleation improves oocyte quality for use in nuclear transfer methodology.
摘要翻译： 本发明涉及以下发现：在去核卵母细胞中存在卵母细胞纺锤体相关因子改善了卵母细胞质量和随后的核转移。 特别是，发现在摘除卵母细胞后维持卵母细胞主轴因子改善了用于核转移方法的卵母细胞质量。
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公开(公告)号：US07592503B2
公开(公告)日：2009-09-22
申请号：US10913013
申请日：2004-08-06
IPC分类号： C12N15/00
CPC分类号： C12N15/8772 , A01K67/0273 , A01K2217/05 , A01K2227/102 , A01K2227/105 , A01K2227/108 , A01K2267/01 , A61K38/00 , C07K14/8121 , C12N15/8509
摘要： The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an activated enucleated oocyte to thereby obtain a nuclear transfer embryo, and impregnating an animal with the nuclear transfer embryo in conditions suitable for gestation of a cloned animal. The invention further relates to methods of chemically enucleating an oocyte having a meiotic spindle apparatus by exposing the oocyte with a compound that destabilizes the meiotic spindle apparatus.
摘要翻译： 本发明涉及克隆动物的方法。 特别地，本发明包括通过将活化的供体细胞的基因组与活化的去核卵母细胞组合来克隆动物的方法，从而获得核转移胚胎，并在适于妊娠克隆的条件下用转移胚胎浸渍动物 动物。 本发明还涉及通过用令愈伤组织主轴装置不稳定的化合物暴露卵母细胞来化学去核卵母细胞的方法。
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公开(公告)号：US20050144663A1
公开(公告)日：2005-06-30
申请号：US10913013
申请日：2004-08-06
IPC分类号： A01K67/027 , A61K38/00 , C07K14/81 , C12N15/85 , C12N15/877 , A61K31/16 , A61K31/337
CPC分类号： C12N15/8772 , A01K67/0273 , A01K2217/05 , A01K2227/102 , A01K2227/105 , A01K2227/108 , A01K2267/01 , A61K38/00 , C07K14/8121 , C12N15/8509
摘要： The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an activated enucleated oocyte to thereby obtain a nuclear transfer embryo, and impregnating an animal with the nuclear transfer embryo in conditions suitable for gestation of a cloned animal. The invention further relates to methods of chemically enucleating an oocyte having a meiotic spindle apparatus by exposing the oocyte with a compound that destabilizes the meiotic spindle apparatus.
摘要翻译： 本发明涉及克隆动物的方法。 特别地，本发明包括通过将活化的供体细胞的基因组与活化的去核卵母细胞组合来克隆动物的方法，从而获得核转移胚胎，并在适于妊娠克隆的条件下用转移胚胎浸渍动物 动物。 本发明还涉及通过用令愈伤组织主轴装置不稳定的化合物暴露卵母细胞来化学去核卵母细胞的方法。
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公开(公告)号：US06781030B1
公开(公告)日：2004-08-24
申请号：US09432906
申请日：1999-11-02
IPC分类号： C12N1500
CPC分类号： C12N15/8772 , A01K67/0273 , A01K2217/05 , A01K2227/102 , A01K2227/105 , A01K2227/108 , A01K2267/01 , A61K38/00 , C07K14/8121 , C12N15/8509
摘要： The present invention pertains to methods for cloning animals. In particular, the invention includes methods of cloning an animal by combining a genome from an activated donor cell with an activated enucleated oocyte to thereby obtain a nuclear transfer embryo, and impregnating an animal with the nuclear transfer embryo in conditions suitable for gestation of a cloned animal. The invention further relates to methods of chemically enucleating an oocyte having a meiotic spindle apparatus by exposing the oocyte with a compound that destabilizes the meiotic spindle apparatus.
摘要翻译： 本发明涉及克隆动物的方法。 特别地，本发明包括通过将活化的供体细胞的基因组与活化的去核卵母细胞组合来克隆动物的方法，从而获得核转移胚胎，并在适于妊娠克隆的条件下用转移胚胎浸渍动物 动物。 本发明还涉及通过用令愈伤组织主轴装置不稳定的化合物暴露卵母细胞来化学去核卵母细胞的方法。
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