利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

6 条记录 (耗时 0.017 秒)

    Common marmoset embryonic stem cell lines
    1.
    发明申请
    Common marmoset embryonic stem cell lines 审中-公开
    普通的m猴胚胎干细胞系

    公开(公告)号：US20060177926A1

    公开(公告)日：2006-08-10

    申请号：US11218699

    申请日：2005-09-02

    申请人： Erika Sasaki ,  Yoshikuni Tanioka ,  Kenzaburo Tani ,  Hideyuki Okano

    发明人： Erika Sasaki ,  Yoshikuni Tanioka ,  Kenzaburo Tani ,  Hideyuki Okano

    IPC分类号： C12N5/06

    CPC分类号： C12N5/0606 ,  C12N2502/13

    摘要： The present invention relates to novel common marmoset (Callithrix jacchus) embryonic stem cell cultures and cell lines, and their use. The common marmoset embryonic cells of the present invention (i) are capable of prolonged undifferentiated proliferation in vitro, (ii) maintain, during prolonged culture, a karyotype in which all the chromosomal characteristics of common marmoset are present without noticeable alteration; (iii) are capable of differentiation into all three embryonic germ layers (ectoderm, endoderm and mesoderm) even after prolonged culture; and (iv) are capable of teratoma formation in vivo. The stem cells preferably are totipotent.

    摘要翻译： 本发明涉及新型普通m猴（Callithrix jacchus）胚胎干细胞培养物和细胞系及其用途。 本发明的普通m猴胚胎细胞（i）能够在体外延长未分化的增殖，（ii）在延长的培养期间保持其中存在所有染色体上的所有染色体特征而不明显改变的核型; （iii）即使在长时间培养后也能够分化成所有三个胚胎胚层（外胚层，内胚层和中胚层）; 和（iv）能够在体内形成畸胎瘤。 干细胞优选是全能的。

    Methods for introducing a human gene into a marmoset embryo for making a transgenic marmoset
    2.
    发明授权
    Methods for introducing a human gene into a marmoset embryo for making a transgenic marmoset 有权
    将人基因导入et et胚用于制备转基因m猴的方法

    公开(公告)号：US08592643B2

    公开(公告)日：2013-11-26

    申请号：US12865304

    申请日：2008-12-09

    申请人： Erika Sasaki ,  Hideyuki Okano

    发明人： Erika Sasaki ,  Hideyuki Okano

    IPC分类号： A01K67/00

    CPC分类号： A01K67/0275 ,  A01K2217/052 ,  A01K2227/106 ,  A01K2267/0318 ,  A01K2267/0356 ,  A01K2267/0393 ,  C07K14/43595 ,  C07K14/47 ,  C12N15/8509 ,  C12N2740/15043 ,  C12N2799/027 ,  C12N2999/007

    摘要： An object of the present invention is to provide a method for introducing a gene into an embryo for production of a human disease model primate animal using a non-human primate animal such as a marmoset. The present invention relates to a method for introducing a foreign gene into an early embryo of a non-human primate animal, which comprises placing early embryos of a non-human primate in a 0.2 M to 0.3 M sucrose solution, so as to increase the volume of the perivitelline spaces, and then injecting a viral vector containing a human foreign gene operably linked to a promoter into the perivitelline spaces of the early embryos.

    摘要翻译： 本发明的目的是提供一种使用非人灵长类动物如for子将基因引入胚胎以产生人类疾病模型灵长类动物的方法。 本发明涉及将外源基因导入非人灵长类动物的早期胚胎的方法，其包括将非人灵长类动物的早期胚胎置于0.2M至0.3M蔗糖溶液中，以增加 然后将包含可操作地连接到启动子的人外源基因的病毒载体注入早期胚胎的周围组织中。

    Method of preserving early mammalian embryos by vitrification
    4.
    发明授权
    Method of preserving early mammalian embryos by vitrification 有权
    通过玻璃化保存早期哺乳动物胚胎的方法

    公开(公告)号：US08293462B2

    公开(公告)日：2012-10-23

    申请号：US12197076

    申请日：2008-08-22

    申请人： Tomoo Eto ,  Erika Sasaki

    发明人： Tomoo Eto ,  Erika Sasaki

    IPC分类号： A01N1/00

    CPC分类号： A01N1/02 ,  A01N1/0221

    摘要： This invention provides a solution for preserving mammalian early embryos or ES cells by vitrification, which comprises, as a base material, a phosphate buffer that exclusively contains 10% to 15% (v/v) propylene glycol as polyhydric alcohol or a phosphate buffer that exclusively contains 10% to 15% (v/v) propylene glycol and 25% to 35% (v/v) ethylene glycol as polyhydric alcohols and further contains 15% to 25% (v/v) Percoll® and 0.2 M to 0.5 M sucrose. This invention also provides a method for preserving mammalian early embryos or ES cells by vitrification using such solution.

    摘要翻译： 本发明提供一种用于通过玻璃化保存哺乳动物早期胚胎或ES细胞的方法，其包含作为基础材料的仅含有10％至15％（v / v）丙二醇作为多元醇或磷酸盐缓冲液的磷酸盐缓冲液， 独特地含有10％至15％（v / v）丙二醇和25％至35％（v / v）乙二醇作为多元醇，并且还含有15％至25％（v / v）Percoll，0.2M至0.5 M蔗糖。 本发明还提供了使用这种溶液通过玻璃化来保存哺乳动物早期胚胎或ES细胞的方法。

    Method of Preserving Early Embryos of Experimental Animals by Vitrification
    5.
    发明申请
    Method of Preserving Early Embryos of Experimental Animals by Vitrification 有权
    通过玻璃化保存实验动物早期胚胎的方法

    公开(公告)号：US20090123905A1

    公开(公告)日：2009-05-14

    申请号：US12197076

    申请日：2008-08-22

    申请人： Tomoo Eto ,  Erika Sasaki

    发明人： Tomoo Eto ,  Erika Sasaki

    IPC分类号： A01N1/02 ,  C12N5/02

    CPC分类号： A01N1/02 ,  A01N1/0221

    摘要： This invention provides a solution for preserving mammalian early embryos or ES cells by vitrification, which comprises, as a base material, a phosphate buffer that exclusively contains 10% to 15% (v/v) propylene glycol as polyhydric alcohol or a phosphate buffer that exclusively contains 10% to 15% (v/v) propylene glycol and 25% to 35% (v/v) ethylene glycol as polyhydric alcohols and further contains 15% to 25% (v/v) Percoll® and 0.2 M to 0.5 M sucrose. This invention also provides a method for preserving mammalian early embryos or ES cells by vitrification using such solution.

    摘要翻译： 本发明提供一种用于通过玻璃化保存哺乳动物早期胚胎或ES细胞的方法，其包含作为基础材料的仅含有10％至15％（v / v）丙二醇作为多元醇或磷酸盐缓冲液的磷酸盐缓冲液， 独特地含有10％至15％（v / v）丙二醇和25％至35％（v / v）乙二醇作为多元醇，并且还含有15％至25％（v / v）Percoll和0.2M 至0.5M蔗糖。 本发明还提供了使用这种溶液通过玻璃化来保存哺乳动物早期胚胎或ES细胞的方法。

    Method of preserving early embryos of experimental animals by vitrification
    6.
    发明申请
    Method of preserving early embryos of experimental animals by vitrification 审中-公开
    通过玻璃化保存实验动物的早期胚胎的方法

    公开(公告)号：US20070087322A1

    公开(公告)日：2007-04-19

    申请号：US11323737

    申请日：2005-12-29

    申请人： Tomoo Eto ,  Erika Sasaki

    发明人： Tomoo Eto ,  Erika Sasaki

    IPC分类号： C12N5/08 ,  C12N5/06 ,  A01N1/02

    CPC分类号： A01N1/02 ,  A01N1/0221

    摘要： This invention provides a solution for preserving mammalian early embryos or ES cells by vitrification, which comprises, as a base material, a phosphate buffer that exclusively contains 10% to 15% (v/v) propylene glycol as polyhydric alcohol or a phosphate buffer that exclusively contains 10% to 15% (v/v) propylene glycol and 25% to 35% (v/v) ethylene glycol as polyhydric alcohols and further contains 15% to 25% (v/v) Percoll® and 0.2 M to 0.5 M sucrose. This invention also provides a method for preserving mammalian early embryos or ES cells by vitrification using such solution.

    摘要翻译： 本发明提供一种用于通过玻璃化保存哺乳动物早期胚胎或ES细胞的方法，其包含作为基础材料的仅含有10％至15％（v / v）丙二醇作为多元醇或磷酸盐缓冲液的磷酸盐缓冲液， 独特地含有10％至15％（v / v）丙二醇和25％至35％（v / v）乙二醇作为多元醇，并且还含有15％至25％（v / v）Percoll和0.2M 至0.5M蔗糖。 本发明还提供了使用这种溶液通过玻璃化来保存哺乳动物早期胚胎或ES细胞的方法。