公开(公告)号：US20210324457A1

公开(公告)日：2021-10-21

申请号：US17271866

申请日：2019-08-27

申请人： Eswar Prasad RAMACHANDRAN IYER ,  Michael SCHNALL-LEVIN ,  Augusto Manuel TENTORI ,  Tarje Sigurd MIKKELSEN ,  Benjamin HINDSON ,  Michael Ybarra LUCERO ,  Rajiv BHARADWAJ ,  Yifeng YIN ,  Zachary BENT ,  Jennifer CHEW ,  Meghan L. F. FREY ,  Linda KVASTAD ,  Joakim LUNDEBERG ,  Eva Gracia VILLACAMPA ,  10x Genomics, Inc.

发明人： Eswar Prasad Ramachandran Iyer ,  Michael Schnall-Levin ,  Augusto Manuel Tentori ,  Tarjei Sigurd Mikkelsen ,  Benjamin Hindson ,  Michael Ybarra Lucero ,  Rajiv Bharadwaj ,  Yifeng Yin ,  Zachary Bent ,  Jennifer Chew ,  Meghan L. F. Frey ,  Linda Kvastad ,  Joakim Lundeberg ,  Eva Gracia Villacampa

IPC分类号： C12Q1/6837 ,  C12Q1/6841

摘要： This disclosure relates to methods for generating spatially barcoded arrays using concentration gradients.

公开(公告)号：US20230203577A1

公开(公告)日：2023-06-29

申请号：US17822117

申请日：2022-08-24

申请人： 10x Genomics, Inc.

发明人： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC分类号： C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806

CPC分类号： C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806 ,  C12Q2563/179 ,  C12Q2537/143 ,  C12Q2525/191

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20220228220A1

公开(公告)日：2022-07-21

申请号：US17462712

申请日：2021-08-31

申请人： 10X GENOMICS, INC.

发明人： Stephane Claude Boutet ,  Michael Ybarra Lucero ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Katherine Pfeiffer ,  Sarah Taylor ,  Niranjan Srinivas

IPC分类号： C12Q1/6886 ,  C12N15/10 ,  G16B30/00 ,  G16B50/00 ,  C12Q1/6881 ,  C12Q1/6804

摘要： The present disclosure provides methods, systems, and compositions for parallel processing of nucleic acid samples. Methods and systems of the present disclosure comprise the use of sample-specific barcode sequences, which facilitate the multiplexing of samples, detection of discrete cell populations within a pooled population, and detection of partitions comprising more than one cell.

公开(公告)号：US20210317524A1

公开(公告)日：2021-10-14

申请号：US17272033

申请日：2019-08-27

申请人： 10x Genomics, Inc.

发明人： Michael Ybarra Lucero ,  Michael Schnall-Levin ,  Tarjei Sigurd Mikkelsen ,  Eswar Prasad Ramachandran Iyer ,  Paul Giresi ,  Benjamin Hindson

IPC分类号： C12Q1/6874

摘要： Methods for determining a location of a feature in a spatial array of features include: providing a spatial array including a plurality of features on a substrate, where a feature of the plurality of features has a capture probe, and where the capture probe has a spatial barcode and a constant sequence; hybridizing a first sequencing probe to the spatial barcode, where the first sequencing probe has a first label and a first nucleotide sequence; obtaining a first image of the first label with the first sequencing probe hybridized to the spatial barcode; determining, based on the first image, a first portion of a sequence of the spatial barcode; and associating the feature with a location in the spatial array based on a location of the first label in the first image.

公开(公告)号：US20210130892A1

公开(公告)日：2021-05-06

申请号：US17084481

申请日：2020-10-29

申请人： 10X Genomics, Inc.

发明人： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC分类号： C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20190323088A1

公开(公告)日：2019-10-24

申请号：US16439568

申请日：2019-06-12

申请人： 10X GENOMICS, INC.

发明人： Stephane Claude Boutet ,  Michael Ybarra Lucero ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Katherine Pfeiffer ,  Sarah Taylor ,  Niranjan Srinivas

IPC分类号： C12Q1/6886 ,  G16B50/00 ,  C12N15/10 ,  G16B30/00

摘要： The present disclosure provides methods, systems, and compositions for parallel processing of nucleic acid samples. Methods and systems of the present disclosure comprise the use of sample-specific barcode sequences, which facilitate the multiplexing of samples, detection of discrete cell populations within a pooled population, and detection of partitions comprising more than one cell.

公开(公告)号：US20230340596A1

公开(公告)日：2023-10-26

申请号：US17933347

申请日：2022-09-19

申请人： 10x Genomics, Inc.

发明人： Michael Schnall-Levin ,  Michael Ybarra Lucero ,  Tarjei Sigurd Mikkelsen ,  Patrik Stahl ,  Jonas Frisen ,  Maja Marklund ,  Enric Llorens Bobadilla

IPC分类号： C12Q1/48 ,  C12Q1/6874 ,  C12Q1/6841 ,  C12Q1/6881 ,  C12Q1/6806

CPC分类号： C12Q1/6881 ,  C12Q1/48 ,  C12Q1/6806 ,  C12Q1/6841 ,  C12Q1/6874 ,  C12Y207/07

摘要： The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

公开(公告)号：US11519033B2

公开(公告)日：2022-12-06

申请号：US17688241

申请日：2022-03-07

申请人： 10x Genomics, Inc.

发明人： Michael Schnall-Levin ,  Michael Ybarra Lucero ,  Tarjei Sigurd Mikkelsen ,  Patrik Stahl ,  Jonas Frisen ,  Maja Marklund ,  Enric Llorens Bobadilla

IPC分类号： C12Q1/6881 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/48 ,  C12Q1/6841

摘要： The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

公开(公告)号：US20210010070A1

公开(公告)日：2021-01-14

申请号：US16876709

申请日：2020-05-18

申请人： 10x Genomics, Inc.

发明人： Michael Schnall-Levin ,  Michael Ybarra Lucero ,  Tarjei Sigurd Mikkelsen ,  Patrik Stahl ,  Jonas Frisen ,  Maja Marklund ,  Enric Llorens

IPC分类号： C12Q1/6841 ,  C12Q1/6837

摘要： The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.

公开(公告)号：US20200002764A1

公开(公告)日：2020-01-02

申请号：US16443638

申请日：2019-06-17

申请人： 10X Genomics, Inc.

发明人： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC分类号： C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806

摘要： The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.