公开(公告)号：US20180327723A1

公开(公告)日：2018-11-15

申请号：US15774134

申请日：2016-11-14

Applicant: Evolva SA

Inventor： Dayal Saran ,  Jorgen Hansen ,  Philippe Prochasson ,  Patrick Roussel ,  Laura Occhipinti ,  Alexei Ouspenski ,  Sumire Honda Malca

IPC: C12N9/10 ,  C12P19/44

CPC classification number: C12N9/1048 ,  A01N43/16 ,  A23L29/035 ,  A61Q13/00 ,  C12N15/70 ,  C12P19/44 ,  C12Y106/02004 ,  C12Y204/00 ,  C12Y402/03073

Abstract: The invention relates to methods for producing glycosylated nootkatol. In particular, a recombinant host comprising a gene encoding a UDP-glycosyltransferase polypeptide capable of glycosylating nootkatol is disclosed. Glycosylation of nootkatol detoxifies nootkatol, allowing for greater production of (glycosylated-)nootkatol, a precursor of nootkatone, and therefore greater production of nootkatone. The invention also relates to methods of converting non-toxic, glycosylated nootkatol produced by a recombinant host to nootkatol, wherein the nootkatol can subsequently be converted to large quantities of nootkatone to be used in flavorings, perfumes, and/or insect repellents.