Beta-Lactone Production Through Bioconversion and Methods Related Thereto

    公开(公告)号:US20240191265A1

    公开(公告)日:2024-06-13

    申请号:US18509425

    申请日:2023-11-15

    CPC classification number: C12P7/62

    Abstract: Methods of β-lactone production, including the integrated bioconversion of β-lactone production and methods related thereto. Methods include reacting a feedstock and a recombinant microorganism harboring one or more genes encoding enzymes involved in the metabolic pathway of a 3-hydroxycarboxylic acid, thereby producing a 3-hydroxycarboxylic acid having C1-C10 alkyl groups. Thereafter, reacting the 3-hydroxycarboxilic acid with a bioengineered natural β-lactone synthetase, the bioengineered β-lactone synthetase engineered to comprise a substrate binding pocket capable of accepting the 3-hydroxycarboxylic acid having C1-C10 alkyl groups, thereby producing a β-lactone having C1-C10 alkyl groups.

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