公开(公告)号：US20180356398A1

公开(公告)日：2018-12-13

申请号：US15618150

申请日：2017-06-09

Applicant: FUJIFILM Corporation ,  The General Hospital Corporation

Inventor： Koju ITO ,  Chihaya KAKINUMA ,  Masafumi NISHINO ,  Shinji MIMA ,  Craig M Neville ,  Cathryn A Sundback

IPC: G01N33/50

CPC classification number: G01N33/5088 ,  G01N33/5023 ,  G01N33/5044 ,  G01N33/5061 ,  G01N33/5064

Abstract: A living tissue model device includes: a first liquid compartment storing a liquid composition; a second liquid compartment storing a liquid composition; and a cell layered body disposed between the first liquid compartment and the second liquid compartment, as a partition between both compartments. A vascular wall model includes: a porous membrane having a honeycomb structure; a vascular endothelial cell layer disposed on one face of the porous membrane; and a smooth muscle cell layer, or a mesenchymal stem cell layer, disposed on another face of the porous membrane. A vascular wall model device includes: a first liquid compartment storing a liquid composition; a second liquid compartment storing a liquid composition; and a vascular wall model disposed between the first liquid compartment and the second liquid compartment, as a partition between both compartments. Applications of these models or model devices are also provided.

公开(公告)号：US20200095531A1

公开(公告)日：2020-03-26

申请号：US16695073

申请日：2019-11-25

Applicant: FUJIFILM CORPORATION

Inventor： Masafumi NISHINO ,  Koju ITO ,  Shinji MIMA ,  Hayato MIYOSHI

IPC: C12M1/12 ,  C12N5/071

Abstract: Provided is a method for producing a cell laminate including cell layers on both surfaces of a porous membrane, using a vessel having a bottom portion and a side wall portion standing from a periphery of the bottom portion, the porous membrane, and a holding member configured to hold the porous membrane such that the porous membrane faces an inner bottom surface of the vessel and is held at a position that does not contact the inner bottom surface, the method including culturing first cells in a liquid medium that contacts the inner bottom surface of the vessel and a surface of the porous membrane, in a state in which the porous membrane is held, by the holding member, at a position that does not contact the inner bottom surface of the vessel so as to face the inner bottom surface, and in which the bottom portion of the vessel is positioned at the upper side while the porous membrane is positioned at the lower side in a direction of gravity; and culturing the first cells at a lower surface of the porous membrane and culturing second cells at an upper surface of the porous membrane, in a state in which the porous membrane is held, by the holding member, at a position that does not contact the inner bottom surface of the vessel so as to face the inner bottom surface, and in which the bottom portion of the vessel is positioned at the lower side while the porous membrane is positioned at the upper side in the direction of gravity.