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公开(公告)号：US11788044B2
公开(公告)日：2023-10-17
申请号：US16820530
申请日：2020-03-16
Applicant: EMULATE, INC.
Inventor： Antonio Varone , Magdalena Kasendra , Carolina Lucchesi , S. Jordan Kerns , Riccardo Barrile , Sonalee Barthakur
CPC classification number: C12M23/16 , B01L3/502715 , B01L3/502761 , C12M23/26 , C12M25/02 , C12N5/069 , G01N33/5047 , G01N33/5064 , B01L2200/16 , B01L2300/123 , B01L2300/16 , C12N2500/00 , C12N2501/052 , C12N2501/2301 , C12N2501/2306 , C12N2501/25
Abstract: The present invention contemplates compositions, devices and methods of simulating biological fluids in a fluidic device, including but not limited to a microfluidic chip. In one embodiment, fluid comprising a colloid under flow in a microfluidic chip has a fluid density or viscosity similar to a bodily fluid, e.g. blood, lymph, lung fluid, or the like. In one embodiment, a fluid is provided as a Theologically biomimetic blood surrogate or substitute for simulating physiological shear stress and cell dynamics in fluidic device, including but not limited to immune cells.
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公开(公告)号：US11701657B2
公开(公告)日：2023-07-18
申请号：US17077828
申请日：2020-10-22
Applicant: Agency for Science, Technology and Research
Inventor： Jackie Y. Ying , Min-Han Tan , Yoke San Daniel Lee , Igor Cima , Yeon Joon Park , Wai Min Phyo
IPC: B01L3/00 , C12N5/071 , G01N33/50 , G01N33/574 , C12Q1/6886
CPC classification number: B01L3/502761 , C12N5/069 , C12Q1/6886 , G01N33/5064 , G01N33/5094 , G01N33/574 , G01N33/57419 , G01N33/57492 , B01L2200/027 , B01L2200/0647 , B01L2300/042 , B01L2300/0681 , C12Q2600/156
Abstract: A column-based device and method for retrieving cells of interest were enclosed. The said device comprises a column comprising (i) an inner wall defining an inner chamber with inlet and outlet openings, (ii) a perforated plug disposed adjacent to the outlet opening, (iii) a sleeve insert with a channel and disposed within the chamber and adjacent to the perforated plug, and (iv) a filtering means housed within sleeve insert sandwiched between two sealing means. In particular, Tumor-derived endothelial cell clusters (TECCs) as characterized multiple nuclei, expression of endothelial markers (PECAM1, VWF and CDH5), and non-expression of leukocyte, megakaryocyte and platelets markers, may be retrieved using the disclosed device. Also encompassed are methods, reagents and kits for the diagnosis and prognosis of cancers by detecting for the presence of TECCs isolated from blood samples using the claimed device.
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公开(公告)号：US20230194502A1
公开(公告)日：2023-06-22
申请号：US18170644
申请日：2023-02-17
Applicant: GENTIUM S.R.L.
Inventor： Terenzio IGNONI , Vijay KUMAR , Claudio VERGA
IPC: G01N33/50
CPC classification number: G01N33/5014 , G01N33/5064
Abstract: The present invention relates to cell-based methods for determining the biological activity of defibrotide. In particular, the invention provides a method for assessing the potency of defibrotide by assessing the viability of mammalian cells in the presence of at least one cytotoxic agent and one or more concentrations of defibrotide. Such methods are particularly useful for standardizing pharmaceutical compositions comprising defibrotide.
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公开(公告)号：US11674123B2
公开(公告)日：2023-06-13
申请号：US15048789
申请日：2016-02-19
Applicant: Wisconsin Alumni Research Foundation
Inventor： James A. Thomson , Jue Zhang
CPC classification number: C12N5/069 , A61K35/44 , C12N5/0031 , C12N5/0056 , G01N33/5064 , C12N2500/35 , C12N2500/90 , C12N2500/98 , C12N2501/10 , C12N2501/113 , C12N2501/15 , C12N2501/155 , C12N2501/16 , C12N2501/165 , C12N2501/415 , C12N2501/42 , C12N2506/02 , C12N2506/45
Abstract: Methods for generating human arterial endothelial cells under defined conditions in the absence of insulin are described. In particular, provided herein are efficient, defined, and scalable methods for generating human arterial endothelial cells from human pluripotent stem cells. Also provided herein are uses of human arterial endothelial cells obtained according to these methods. For example, methods of treating peripheral arterial disease and methods of screening agents for that effect adhesion of leukocytes to arterial endothelial cells are also provided.
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公开(公告)号：US20190195889A1
公开(公告)日：2019-06-27
申请号：US16331483
申请日：2017-08-03
Applicant: Henkel AG & Co. KGaA
Inventor： Patricia Klaka , Bernhard Banowski , Sabine Gruedl , Thomas Welss , Melanie Giesen
CPC classification number: G01N33/6881 , C12N5/0629 , C12N5/0633 , C12N2503/02 , C12N2503/06 , C12N2513/00 , G01N33/5064 , G01N33/5082
Abstract: The present disclosure relates to an in-vitro method for identifying and analyzing ion channels and/or water channels and/or receptors of signal transduction, in which a three-dimensional sweat gland equivalent having from about 500 to about 500,000 sweat gland cells and a diameter of from about 100 to about 6,000 μm is firstly provided and then any ion channels and/or water channels and/or receptors of signal transduction present in this equivalent are infected and analysed. In a further method step c) the influence of test substances on the proteins identified previously in step b) is examined. Since the three-dimensional sweat gland equivalents used in step a) comprise differently differentiated cells and portray the in-vivo situation well, the measurement data obtained with the in-vitro method as contemplated herein can be transferred well to the in-vivo situation.
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Patent Agency Ranking
公开(公告)号：US20180207238A1
公开(公告)日：2018-07-26
申请号：US15746484
申请日：2016-07-29
Applicant: University of Virginia Patent Foundation
Inventor： Brant E. Isakson
CPC classification number: A61K38/2013 , A61K38/1709 , A61P7/00 , A61P29/00 , C07K14/435 , C07K2319/10 , G01N33/5064 , G01N2800/328
Abstract: Inflammatory cell recruitment to local sites of tissue injury and/or infection is controlled by many signaling processes influencing cell-to-cell interactions between vascular endothelial cells (EC) in post-capillary venules and circulating leukocytes. Here we report that the ATP-release channel Pannexin1 (Panx1) opens downstream of EC activation by tumor necrosis factor α (TNF α). This process involves activation of Type 1 TNF receptors, recruitment of Src Family Kinases (SFK), and SFK-dependent phosphorylation of Panx1. We report a previously unidentified role for Panx1 channels in promoting leukocyte adhesion and emigration through the venous wall during acute systemic inflammation. The present application further discloses that Panx IL2 peptide consisting of amino acid sequence KYPIVEQYLKYGRKKQRR (SEQ ID NO: 3) or 10Panx1 peptide consisting of amino acid sequence WRQAAFVDSY (SEQ ID NO: 8) are inhibitors of leukocyte adhesion.
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公开(公告)号：US20180011103A1
公开(公告)日：2018-01-11
申请号：US15661241
申请日：2017-07-27
Applicant: Janssen Diagnostics, LLC , Pfizer Inc.
Inventor： Antonio GUALBERTO , Maria Luisa PACCAGNELLA , Carrie L. MELVIN , Madeline I. REPOLLET , David CHIANESE , Mark Carle CONNELLY , Leonardus Wendelinus Mathias Marie TERSTAPPEN
IPC: G01N33/574 , G01N33/50 , G01N30/02
CPC classification number: G01N33/57496 , G01N30/02 , G01N33/5023 , G01N33/5064 , G01N33/574 , G01N33/57492 , G01N2333/4742 , G01N2333/4745 , G01N2333/71 , G01N2333/91205 , G01N2458/00 , G01N2800/52 , B01D15/3804
Abstract: Methods for the detection, enumeration and analysis of circulating tumor cells expressing insulin-like growth factor-1 receptors (IGF-1R) are disclosed. These methods are useful for cancer screening and staging, development of treatment regimens, and for monitoring for treatment responses, cancer recurrence or the like. Test kits that facilitate the detection, enumeration and analysis of such circulating tumor cells are also provided.
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公开(公告)号：US09694338B2
公开(公告)日：2017-07-04
申请号：US14575084
申请日：2014-12-18
Applicant: Wisconsin Alumni Research Foundation
Inventor： William Murphy , Ngoc Nhi Le
CPC classification number: B01J19/0046 , B01J2219/00315 , B01J2219/0059 , B01J2219/00626 , B01J2219/00637 , B01J2219/00644 , B01J2219/00659 , B01J2219/00675 , B01J2219/00711 , B01J2219/00725 , B01J2219/00736 , B01J2219/00756 , G01N33/5026 , G01N33/5032 , G01N33/5064 , G01N33/5073
Abstract: Hydrogel arrays, methods for preparing hydrogel arrays and methods for screening cell-substrate interactions using the hydrogel arrays are disclosed. Advantageously, the hydrogel arrays include individual hydrogel posts that are completely isolatable, allowing for systematic and independent control of the chemical composition and physical dimensions of each hydrogel post.
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公开(公告)号：US09597360B2
公开(公告)日：2017-03-21
申请号：US14866598
申请日：2015-09-25
Inventor： Misako Nakashima , Hiroshi Nakamura
CPC classification number: A61K35/32 , A61K6/0017 , A61K6/0067 , A61K6/02 , A61K35/36 , A61K35/44 , A61K38/4886 , G01N33/5023 , G01N33/5064 , G01N33/5073 , G01N2333/96494 , G01N2800/18
Abstract: A method of treatment for pulpitis and/or enhancement for dentinogenesis, includes using dental materials that include at least one of a protein having matrix metalloprotease 3 activities or matrix metalloprotease 3 precursor protein as an active ingredient. The dental materials can also contain a carrier having biocompatibility, and can contain at least one cell type among pulp cells, pulp stem cells, pulp progenitor cells, odontoblasts or cells that can differentiate into odontoblasts.
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公开(公告)号：US20170059557A1
公开(公告)日：2017-03-02
申请号：US15253650
申请日：2016-08-31
Applicant: The Regents of The University of California
Inventor： Elliot L. Botvinick , Vasan Venugopalan , Jonathan Compton , Amy Hellman
IPC: G01N33/50
CPC classification number: G01N33/5032 , C40B30/06 , G01N33/5064 , G01N33/54373
Abstract: A method and device are disclosed for inducing mechanical stress in a cellular sample to evaluate mechanotransduction in the cellular sample. In one embodiment, the mechanical stress is induced by generating a microcavitation bubble in the cellular sample using a pulsed energy. The microcavitation bubble creates a microtsunami, which provides a transient, impulsive mechanical stress on the cellular sample, forming a gradient of effects at distances away from the microcavitation bubble.
Abstract translation: 公开了用于在细胞样品中诱导机械应力以评估细胞样品中的机械转导的方法和装置。 在一个实施例中，通过使用脉冲能量在细胞样品中产生微气泡来诱导机械应力。 微气泡产生微孔，其在细胞样品上提供瞬时的脉冲机械应力，在远离微气泡的距离处形成效应的梯度。
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