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    CELL CONSTRUCT FOR CELL TRANSPLANTATION, BIOCOMPATIBLE POLYMER BLOCK, AND METHOD FOR PRODUCING THE SAME
    3.
    发明申请
    CELL CONSTRUCT FOR CELL TRANSPLANTATION, BIOCOMPATIBLE POLYMER BLOCK, AND METHOD FOR PRODUCING THE SAME 审中-公开
    用于细胞移植的细胞结构，生物成分聚合物块及其生产方法

    公开(公告)号：US20150352252A1

    公开(公告)日：2015-12-10

    申请号：US14836504

    申请日：2015-08-26

    Applicant: FUJIFILM CORPORATION

    Inventor： Kentaro NAKAMURA ,  Reiko IWAZAWA ,  Hayato MIYOSHI ,  Kazuhiro YAMAGUCHI ,  Hideo FUSHIMI

    IPC: A61L27/22 ,  A61L27/38 ,  A61L27/36

    CPC classification number: A61L27/222 ,  A61L27/3604 ,  A61L27/3608 ,  A61L27/38 ,  A61L27/3834 ,  A61L27/3886 ,  A61L27/56 ,  A61L27/58 ,  A61L2300/64 ,  A61L2430/22 ,  C12N5/0068 ,  Y10T428/2982

    Abstract: It is an object of the present invention to provide a cell construct for cell transplantation that does not contain a substance having cytotoxicity, such as glutaraldehyde, and suppresses the necrosis of the transplanted cells in the construct (namely, having a high cell survival rate). The present invention provides a cell construct for cell transplantation comprising biocompatible polymer blocks that do not contain glutaraldehyde and at least one type of cells, wherein a plurality of biocompatible polymer blocks are disposed in gaps among a plurality of cells, and wherein the biocompatible polymer blocks have a tap density of 10 mg/cm3 or more and 500 mg/cm3 or less, or the value of the square root of the cross-sectional area/boundary length in the two-dimensional sectional image of the polymer block is 0.01 or more and 0.13 or less.

    Abstract translation: 本发明的目的是提供一种不含有细胞毒性的物质的细胞移植用细胞构建体，例如戊二醛，抑制构建体中移植细胞的坏死（即具有高细胞存活率） 。 本发明提供了一种用于细胞移植的细胞构建体，其包含不含戊二醛和至少一种类型细胞的生物相容性聚合物嵌段，其中多个生物相容性聚合物嵌段设置在多个细胞之间的间隙中，并且其中生物相容性聚合物嵌段 具有10毫克/厘米3以上至500毫克/厘米3以下的振实密度，或聚合物嵌段的二维截面图中的截面积/边界长度的平方根的值为0.01以上 和0.13以下。

    MICROCHANNEL DEVICE
    4.
    发明申请
    MICROCHANNEL DEVICE 审中-公开

    公开(公告)号：US20200369998A1

    公开(公告)日：2020-11-26

    申请号：US16986259

    申请日：2020-08-06

    Applicant: FUJIFILM CORPORATION

    Inventor： Hayato MIYOSHI ,  Koju ITO ,  Takahiro OBA ,  Akira WAKABAYASHI

    IPC: C12M3/06 ,  C12M1/12 ,  B81B1/00

    Abstract: Provided is a microchannel device including a first microchannel that is formed in a first channel member, a second microchannel that is formed in a second channel member and at least a portion of which overlaps the first microchannel in plan view with a step portion formed between the first microchannel and the second microchannel, a porous membrane that has a plurality of holes penetrating the porous membrane in a thickness direction and is disposed between the first channel member and the second channel member to partition the first microchannel and the second microchannel, and a reinforcing member that is provided between the first channel member or the second channel member and the porous membrane, is higher in stiffness than the porous membrane, and reinforces at least a portion of the porous membrane that faces the step portion.

    PRODUCTION METHOD OF MYOCARDIAL CELL LAYER, MYOCARDIAL CELL LAYER, AND USE THEREOF
    7.
    发明公开
    PRODUCTION METHOD OF MYOCARDIAL CELL LAYER, MYOCARDIAL CELL LAYER, AND USE THEREOF 审中-公开

    公开(公告)号：US20240277899A1

    公开(公告)日：2024-08-22

    申请号：US18648087

    申请日：2024-04-26

    Applicant: FUJIFILM Corporation ,  OSAKA UNIVERSITY

    Inventor： Hayato MIYOSHI ,  Li LIU ,  Yoshiki SAWA ,  Junjun LI ,  Shigeru MIYAGAWA ,  Maki TAKEDA

    IPC: A61L27/38 ,  A61K35/34 ,  C12N5/077

    CPC classification number: A61L27/38 ,  A61K35/34 ,  C12N5/0657 ,  C12N2502/1323

    Abstract: An object of the present invention is to provide a production method of a myocardial cell layer, and a myocardial cell layer, in which myocardial cells can be matured without using a complicated control device and can be maintained for a long period of time, and to provide a kit for evaluating a pharmaceutical candidate substance, a transplantation material, and an evaluation method of a pharmaceutical candidate substance. According to the present invention, there is provided a production method of a myocardial cell layer including the following steps (1) to (3).



    (1) seeding a myocardial cell induced from a pluripotent stem cell in a single layer on a seeding surface having a shape shown in the following (1-1) to form a myocardial cell layer,
    (1-1) in a case where spread of the seeding surface in a horizontal direction is a plane figure, a shape thereof is a shape obtained by hollowing out at least a part of a first plane figure of an optional shape having uniform spread in a second plane figure of another optional shape having uniform spread without contacting an outer periphery of the first plane figure of the optional shape, where an average value of a length of the outer periphery of the first plane figure and a length of an outer periphery of the second plane figure satisfies the following formula;










    in the formula, x represents the average value of the length of the outer periphery of the first plane figure and the length of the outer periphery of the second plane figure, where a unit thereof is cm, and v represents a conduction velocity of the myocardial cell induced from the pluripotent stem cell, where a unit thereof is cm·s−1,
    (2) circulating and proceeding an electrical signal or a calcium signal in the myocardial cell layer, and
    (3) culturing the cells for 8 days or more while maintaining a state of the (2).

    METHOD FOR PRODUCING CELL LAMINATE
    10.
    发明申请
    METHOD FOR PRODUCING CELL LAMINATE 审中-公开

    公开(公告)号：US20200095531A1

    公开(公告)日：2020-03-26

    申请号：US16695073

    申请日：2019-11-25

    Applicant: FUJIFILM CORPORATION

    Inventor： Masafumi NISHINO ,  Koju ITO ,  Shinji MIMA ,  Hayato MIYOSHI

    IPC: C12M1/12 ,  C12N5/071

    Abstract: Provided is a method for producing a cell laminate including cell layers on both surfaces of a porous membrane, using a vessel having a bottom portion and a side wall portion standing from a periphery of the bottom portion, the porous membrane, and a holding member configured to hold the porous membrane such that the porous membrane faces an inner bottom surface of the vessel and is held at a position that does not contact the inner bottom surface, the method including culturing first cells in a liquid medium that contacts the inner bottom surface of the vessel and a surface of the porous membrane, in a state in which the porous membrane is held, by the holding member, at a position that does not contact the inner bottom surface of the vessel so as to face the inner bottom surface, and in which the bottom portion of the vessel is positioned at the upper side while the porous membrane is positioned at the lower side in a direction of gravity; and culturing the first cells at a lower surface of the porous membrane and culturing second cells at an upper surface of the porous membrane, in a state in which the porous membrane is held, by the holding member, at a position that does not contact the inner bottom surface of the vessel so as to face the inner bottom surface, and in which the bottom portion of the vessel is positioned at the lower side while the porous membrane is positioned at the upper side in the direction of gravity.

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