公开(公告)号：US20150050737A1

公开(公告)日：2015-02-19

申请号：US14528197

申请日：2014-10-30

Applicant: FUJIFILM CORPORATION

Inventor： Yuta MURAKAMI ,  Rie IWATA ,  Yoshihide IWAKI ,  Tasuku SASAKI

IPC: C12N5/074 ,  C07K14/00

CPC classification number: C12N5/0068 ,  C07K14/001 ,  C07K14/47 ,  C07K14/78 ,  C12N5/0607 ,  C12N5/0696 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2537/00

Abstract: A polypeptide including: (1) a first region containing at least one selected from the group consisting of an amino acid sequence represented by CSYYQSC (SEQ ID NO:1) and an amino acid sequence represented by RGD; and (2) a second region containing (2-i) an amino acid sequence represented by PRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQN (SEQ ID NO:2), (2-ii) an amino acid sequence having an identity of not less than 50% to the amino acid sequence represented by SEQ ID NO:2 and having an adsorption ability to a cultivation container, or (2-iii) an amino acid sequence that is the amino acid sequence represented by SEQ ID NO:2 in which from 1 to 30 amino acid residues are added, substituted, or deleted, and has an adsorption ability to a cultivation container, in which the polypeptide includes from 40 to 450 amino acid residues.

Abstract translation: 一种多肽，包括：（1）含有选自由CSYYQSC（SEQ ID NO：1）表示的氨基酸序列和由RGD表示的氨基酸序列组成的组中的至少一种的第一区域; 和（2）含有（2-i）由PRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQN（SEQ ID NO：2）表示的氨基酸序列的第二区域，（2-ii）与氨基酸具有不小于50％的同一性的氨基酸序列 由SEQ ID NO：2表示的具有对培养容器的吸附能力的序列，或（2-iii）作为SEQ ID NO：2所示的氨基酸序列的氨基酸序列，其中含有1〜30个氨基酸残基 被添加，取代或缺失，并且对培养容器具有吸附能力，其中多肽包含40至450个氨基酸残基。

公开(公告)号：US20170022473A1

公开(公告)日：2017-01-26

申请号：US15291246

申请日：2016-10-12

Applicant: FUJIFILM Corporation

Inventor： Yuta MURAKAMI ,  Rie IWATA ,  Yoshihide IWAKI ,  Tasuku SASAKI

IPC: C12N5/00 ,  C07K14/00 ,  C12N5/074

CPC classification number: C12N5/0068 ,  C07K14/001 ,  C07K14/47 ,  C07K14/78 ,  C12N5/0607 ,  C12N5/0696 ,  C12N2533/50 ,  C12N2533/52 ,  C12N2537/00

Abstract: A polypeptide including: (1) a first region containing at least one selected from the group consisting of an amino acid sequence represented by CSYYQSC (SEQ ID NO:1) and an amino acid sequence represented by RGD; and (2) a second region containing (2-i) an amino acid sequence represented by PRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQN (SEQ ID NO:2), (2-ii) an amino acid sequence having an identity of not less than 50% to the amino acid sequence represented by SEQ ID NO:2 and having an adsorption ability to a cultivation container, or (2-iii) an amino acid sequence that is the amino acid sequence represented by SEQ ID NO:2 in which from 1 to 30 amino acid residues are added, substituted, or deleted, and has an adsorption ability to a cultivation container, in which the polypeptide includes from 40 to 450 amino acid residues.

Abstract translation: 一种多肽，包括：（1）含有选自由CSYYQSC（SEQ ID NO：1）表示的氨基酸序列和由RGD表示的氨基酸序列组成的组中的至少一种的第一区域; 和（2）含有（2-i）由PRPSLAKKQRFRHRNRKGYRSQRGHSRGRNQN（SEQ ID NO：2）表示的氨基酸序列的第二区域，（2-ii）与氨基酸具有不小于50％的同一性的氨基酸序列 由SEQ ID NO：2表示的具有对培养容器的吸附能力的序列，或（2-iii）作为SEQ ID NO：2所示的氨基酸序列的氨基酸序列，其中含有1〜30个氨基酸残基 被添加，取代或缺失，并且对培养容器具有吸附能力，其中多肽包含40至450个氨基酸残基。

公开(公告)号：US20150175969A1

公开(公告)日：2015-06-25

申请号：US14640218

申请日：2015-03-06

Applicant: FUJIFILM Corporation

Inventor： Yuichi YOSHINO ,  Rie IWATA ,  Kentaro NAKAMURA

IPC: C12N5/077 ,  C07K14/78

CPC classification number: C12N5/0655 ,  A61K38/00 ,  A61L27/227 ,  A61L2300/252 ,  A61L2430/06 ,  C07K14/78 ,  C12N2501/998

Abstract: A polypeptide having an amino acid sequence in which the number of RGD sequences contained per molecular weight of 10 kDa is not less than 0.30; the number of GFPGER sequences contained per molecular weight of 10 kDa is not less than 0.15; and the number of GVMGFP sequences contained per molecular weight of 10 kDa is less than 0.30; is provided. A scaffold composition, a composition for repairing a cartilage tissue, a composition for culturing cartilage cells, and a composition for promoting glycosaminoglycan production, which compositions contain the above polypeptide, are also provided.

Abstract translation: 具有每分子量为10kDa的RGD序列数不少于0.30的氨基酸序列的多肽; 每分子量为10kDa的GFPGER序列数不低于0.15; 每分子量为10kDa的GVMGFP序列数小于0.30; 被提供。 还提供了一种支架组合物，用于修复软骨组织的组合物，用于培养软骨细胞的组合物和用于促进糖胺聚糖生产的组合物，该组合物含有上述多肽。