公开(公告)号：US20190264258A1

公开(公告)日：2019-08-29

申请号：US16370329

申请日：2019-03-29

Applicant: FUJIFILM Corporation

Inventor： Takayuki Tsujimoto ,  Yasuyuki Ishii ,  Yuki Inoue ,  Aya Ouchi ,  Toshiyuki Nakatani ,  Setsu Endoh

IPC: C12Q1/686 ,  C12Q1/6853 ,  C12Q1/6806 ,  G16B30/00 ,  C12Q1/6886

Abstract: Provided is a method for obtaining base sequence information of a single cell derived from a vertebrate, by which PCR amplification of an objective region can be performed uniformly and accurately.

公开(公告)号：US11738338B2

公开(公告)日：2023-08-29

申请号：US16950434

申请日：2020-11-17

Applicant: FUJIFILM Corporation

Inventor： Takeshi Hama ,  Noboru Komori ,  Yuki Inoue ,  Aya Ouchi

IPC: B01L3/00 ,  G01N33/543

CPC classification number: B01L3/502715 ,  G01N33/54366 ,  B01L2300/123 ,  B01L2400/0406

Abstract: A test container including: at least two accommodation portions each accommodating a liquid and internally provided; a flow path connecting the two accommodation portions to each other at respective upper end positions thereof internally provided; and a flexible film deformable inwards of at least one accommodation portion at a portion forming an upper wall surface of the one accommodation portion, in which the liquid accommodated in the one accommodation portion is fed to the other accommodation portion via the flow path due to deformation of the flexible film towards the one accommodation portion, and a breaking elongation of the flexible film is 100% or more and 600% or less.

公开(公告)号：US11511272B2

公开(公告)日：2022-11-29

申请号：US16952198

申请日：2020-11-19

Applicant: FUJIFILM Corporation

Inventor： Takeshi Hama ,  Noboru Komori ,  Yuki Inoue ,  Aya Ouchi

IPC: B01L3/00

Abstract: A test container includes a container main body including a first-accommodation-portion, a second-accommodation-portion, and a third-accommodation-portion each accommodating a liquid and internally provided, a first flow path connecting the first-accommodation-portion and the second-accommodation-portion to each other at respective upper end positions thereof and internally provided, and a second flow path connecting the second-accommodation-portion and the third-accommodation-portion to each other at respective upper end positions thereof and internally provided, in which at least a portion forming an upper wall surface of the second-accommodation-portion has flexibility to be deformable inwards of the second-accommodation-portion; and a liquid return prevention structure which prevents a backflow of the liquid to the first-accommodation-portion, when the liquid accommodated in the second-accommodation-portion is fed to the third-accommodation-portion via the second flow path due to deformation of the portion forming the upper wall surface of the second-accommodation-portion inwards of the second-accommodation-portion.

公开(公告)号：US11306358B2

公开(公告)日：2022-04-19

申请号：US15712785

申请日：2017-09-22

Applicant: FUJIFILM Corporation

Inventor： Aya Ouchi ,  Takayuki Tsujimoto ,  Masaya Nagase ,  Yasuyuki Ishii

IPC: C12Q1/68 ,  C12Q1/6883 ,  C12Q1/6811 ,  C12Q1/6876 ,  C12N15/10 ,  C12N15/09

Abstract: Provided is a method for determining a genetic condition of a fetus, the method including: an objective region selection step of selecting an objective region for determining the genetic condition, from regions on a human genome; a step of isolating a single cell from a maternal blood sample; a step of extracting genomic DNA from the single cell; a step of performing PCR amplification on the objective region using a primer set designed so as to perform the PCR amplification on the objective region using genomic DNA extracted from the single cell as a template; and a DNA sequencing step of decoding a DNA base sequence of a PCR amplification product of the objective region, in which the primer set designed so as to perform the PCR amplification on the objective region is designed through a method for designing a primer set used for the polymerase chain reaction, the method for designing a primer set including a first stage selection step based on a local alignment score and a second stage selection step based on a global alignment score.

公开(公告)号：US11618039B2

公开(公告)日：2023-04-04

申请号：US17034040

申请日：2020-09-28

Applicant: FUJIFILM Corporation

Inventor： Yoshihisa Usami ,  Yasuyuki Ishii ,  Yuki Inoue ,  Aya Ouchi

IPC: B03C1/08 ,  B01D29/44 ,  B03C1/033 ,  B03C1/28 ,  B03C1/30 ,  B01D35/06

Abstract: A separation device includes: a container that includes a storage portion for storing liquid containing magnetic particles; and a coil that separates the magnetic particles from the liquid by generating a magnetic field through application of current, causing a magnetic force to act on the magnetic particles in a state where the coil stops with respect to the storage portion, and causing the magnetic particles to move from the storage portion against gravity acting on the liquid containing the magnetic particles.

公开(公告)号：US11541387B2

公开(公告)日：2023-01-03

申请号：US16952198

申请日：2020-11-19

Applicant: FUJIFILM Corporation

Inventor： Takeshi Hama ,  Noboru Komori ,  Yuki Inoue ,  Aya Ouchi

IPC: B01L3/00

Abstract: A test container includes a container main body including a first-accommodation-portion, a second-accommodation-portion, and a third-accommodation-portion each accommodating a liquid and internally provided, a first flow path connecting the first-accommodation-portion and the second-accommodation-portion to each other at respective upper end positions thereof and internally provided, and a second flow path connecting the second-accommodation-portion and the third-accommodation-portion to each other at respective upper end positions thereof and internally provided, in which at least a portion forming an upper wall surface of the second-accommodation-portion has flexibility to be deformable inwards of the second-accommodation-portion; and a liquid return prevention structure which prevents a backflow of the liquid to the first-accommodation-portion, when the liquid accommodated in the second-accommodation-portion is fed to the third-accommodation-portion via the second flow path due to deformation of the portion forming the upper wall surface of the second-accommodation-portion inwards of the second-accommodation-portion.

公开(公告)号：US11448332B2

公开(公告)日：2022-09-20

申请号：US17035220

申请日：2020-09-28

Applicant: FUJIFILM Corporation

Inventor： Yoshihisa Usami ,  Yasuyuki Ishii ,  Yuki Inoue ,  Aya Ouchi

IPC: F16K17/38 ,  B01F25/00 ,  F16K99/00 ,  G01N35/08 ,  B01L7/00 ,  G01N37/00 ,  B01L3/00

Abstract: A chip includes a first chamber that stores a fluid, a second chamber that stores a mixing target which is to be mixed with the fluid, a flow path that allows a communication between the first chamber and the second chamber, and a valve that is provided in the flow path and capable of changing the flow path by change in shape by heating.