公开(公告)号：US20240425826A1

公开(公告)日：2024-12-26

申请号：US18827497

申请日：2024-09-06

Applicant: FUJIFILM Corporation

Inventor： Taichi MURAGUCHI ,  Yusuke MORI ,  Sayako UMETANI

IPC: C12N7/00

Abstract: An object of the present invention is to provide a producer cell for AAV production, in which a cell injury at the time of establishing a cell strain is avoided or suppressed, a manufacturing method of the producer cell, and a manufacturing method of an adeno-associated virus using the producer cell. According to the present invention, there is provided a producer cell containing, in a chromosome, a Cap gene under a control of an exogenous promoter and a Rep gene under a control of an exogenous promoter, in which the producer cell does not contain at least one of a VA-RNA gene or an E4 gene, and the producer cell is a mammalian cell.

公开(公告)号：US20240018547A1

公开(公告)日：2024-01-18

申请号：US18448499

申请日：2023-08-11

Applicant: FUJIFILM Corporation

Inventor： Yusuke Mori ,  Taichi MURAGUCHI ,  Sayako UMETANI ,  Yuya WATANABE

IPC: C12N15/86 ,  C12N7/02

CPC classification number: C12N15/86 ,  C12N7/02 ,  C12N2750/14143

Abstract: An object of the present invention is to provide a kit and an integration vector for preparing an adeno-associated virus-producing cell capable of controlled manufacture of an adeno-associated virus, and to provide an adeno-associated virus-producing cell capable of controlled manufacture of an adeno-associated virus, a manufacturing method of the same, and a manufacturing method of the adeno-associated virus. According to the present invention, a kit including a first vector containing one or more virus helper genes, in which at least one of the virus helper genes is under a control of a promoter capable of regulating expression, and a second vector containing one or more adeno-associated virus genes, in which at least one of the adeno-associated virus genes is not under a control of a promoter regulating expression is provided.

公开(公告)号：US20240263149A1

公开(公告)日：2024-08-08

申请号：US18621693

申请日：2024-03-29

Applicant: FUJIFILM Corporation

Inventor： Yusuke MORI ,  Sayako UMETANI ,  Taichi MURAGUCHI

IPC: C12N7/00 ,  C12N15/85

CPC classification number: C12N7/00 ,  C12N15/85 ,  C12N2750/14122 ,  C12N2750/14151 ,  C12N2830/50 ,  C12N2830/60

Abstract: An object of the present invention is to provide a manufacturing method of an adeno-associated virus, which can improve a production amount of the adeno-associated virus, and to provide a cell and an expression vector for use in the manufacturing method of an adeno-associated virus. According to the present invention, provided is a manufacturing method of an adeno-associated virus, including expressing an E40RF6 gene with an exogenous promoter, in which expression of E40RF6 is reduced as compared with a case where E40RF6 is expressed using an expression unit containing an enhancer, a cytomegalovirus-derived promoter, a Kozak sequence, an E40RF6 gene, and a polyA signal sequence.