公开(公告)号：US20240425826A1

公开(公告)日：2024-12-26

申请号：US18827497

申请日：2024-09-06

Applicant: FUJIFILM Corporation

Inventor： Taichi MURAGUCHI ,  Yusuke MORI ,  Sayako UMETANI

IPC: C12N7/00

Abstract: An object of the present invention is to provide a producer cell for AAV production, in which a cell injury at the time of establishing a cell strain is avoided or suppressed, a manufacturing method of the producer cell, and a manufacturing method of an adeno-associated virus using the producer cell. According to the present invention, there is provided a producer cell containing, in a chromosome, a Cap gene under a control of an exogenous promoter and a Rep gene under a control of an exogenous promoter, in which the producer cell does not contain at least one of a VA-RNA gene or an E4 gene, and the producer cell is a mammalian cell.

公开(公告)号：US20220228112A1

公开(公告)日：2022-07-21

申请号：US17707215

申请日：2022-03-29

Applicant: FUJIFILM Corporation

Inventor： Yusuke MORI ,  Tadanori YAMADA

IPC: C12N5/071

Abstract: An object of the present invention is to provide a medium with which hepatocytes can be cultured while a metabolic function thereof is maintained, a method of producing a hepatocyte using the above medium, and the hepatocyte that is obtained by the above method. According to the present invention, there is provided a medium for culturing a hepatocyte, containing one or more of a ROCK inhibitor, nicotinamide, and an analogous substance of the nicotinamide, in which a content of a serum component is less than 5% by volume, a content of an EGF receptor activating factor is less than 1 ng/mL, and a content of an ALK inhibitor is less than 0.1 μmol/L.

公开(公告)号：US20240263149A1

公开(公告)日：2024-08-08

申请号：US18621693

申请日：2024-03-29

Applicant: FUJIFILM Corporation

Inventor： Yusuke MORI ,  Sayako UMETANI ,  Taichi MURAGUCHI

IPC: C12N7/00 ,  C12N15/85

CPC classification number: C12N7/00 ,  C12N15/85 ,  C12N2750/14122 ,  C12N2750/14151 ,  C12N2830/50 ,  C12N2830/60

Abstract: An object of the present invention is to provide a manufacturing method of an adeno-associated virus, which can improve a production amount of the adeno-associated virus, and to provide a cell and an expression vector for use in the manufacturing method of an adeno-associated virus. According to the present invention, provided is a manufacturing method of an adeno-associated virus, including expressing an E40RF6 gene with an exogenous promoter, in which expression of E40RF6 is reduced as compared with a case where E40RF6 is expressed using an expression unit containing an enhancer, a cytomegalovirus-derived promoter, a Kozak sequence, an E40RF6 gene, and a polyA signal sequence.