公开(公告)号：US20170146514A1

公开(公告)日：2017-05-25

申请号：US15424101

申请日：2017-02-03

Applicant: FUJIFILM Corporation

Inventor： Kenta MATSUBARA ,  Shinichiro FUKUI ,  Yasuyuki ISHII

IPC: G01N33/49 ,  G01N21/59 ,  G01N15/14

CPC classification number: G01N33/491 ,  G01N15/1434 ,  G01N21/27 ,  G01N21/59 ,  G01N33/48 ,  G01N33/483 ,  G01N33/49 ,  G01N33/4925 ,  G01N33/80 ,  G01N2015/0073 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2201/12

Abstract: Provided is a method for sorting nucleated red blood cells in which fetus-derived nucleated red blood cells are sorted out from maternal blood. The method includes a nucleated red blood cell specification step of specifying nucleated red blood cells to be sorted out; a reference red blood cell selection step of setting a search range including the nucleated red blood cells and selecting red blood cells having no nucleus within the search range as reference red blood cells; and a sorting step of sorting the nucleated red blood cells into mother-derived nucleated red blood cells and fetus-derived nucleated red blood cells in accordance with spectral characteristics attributable to oxygen affinity of hemoglobin of the nucleated red blood cells and the reference red blood cells.

公开(公告)号：US20170146558A1

公开(公告)日：2017-05-25

申请号：US15424302

申请日：2017-02-03

Applicant: FUJIFILM Corporation

Inventor： Yasuyuki ISHII ,  Kenta MATSUBARA ,  Shinichiro FUKUI

IPC: G01N33/80 ,  C12Q1/68 ,  G01N21/31

CPC classification number: G01N33/80 ,  C12Q1/02 ,  C12Q1/68 ,  C12Q1/6883 ,  G01N21/27 ,  G01N21/31 ,  G01N33/48 ,  G01N33/483 ,  G01N33/49

Abstract: A method for sorting nucleated red blood cells, the method includes: selecting a candidate cell of nucleated red blood cells in accordance with shapes of cells; setting a search range including the candidate cell and selecting a white blood cell within the search range; defining that the candidate cell is a nucleated red blood cell through comparison of spectral characteristics of hemoglobin between the candidate cell and the white blood cell; and sorting the nucleated red blood cells into mother-derived nucleated red blood cells or fetus-derived nucleated red blood cells through comparison of an allele contained in the nucleated red blood cell after the defining of the nucleated red blood cell with an allele contained in a white blood cell which has not been selected in the selecting of the candidate cell or in the white blood cell selected in the selecting of the white blood cell.

公开(公告)号：US20170145512A1

公开(公告)日：2017-05-25

申请号：US15423998

申请日：2017-02-03

Applicant: FUJIFILM Corporation

Inventor： Shinichiro FUKUI ,  Yasuyuki ISHII ,  Kenta MATSUBARA

IPC: C12Q1/68 ,  G01N21/31 ,  G01N33/49

CPC classification number: C12Q1/6883 ,  C12Q1/04 ,  C12Q1/68 ,  G01N1/4077 ,  G01N15/1475 ,  G01N21/27 ,  G01N21/31 ,  G01N33/48 ,  G01N33/49 ,  G01N2001/4083 ,  G01N2015/0073 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2021/3133

Abstract: A method for inspecting a chromosome of a fetus includes: a collection step of obtaining maternal blood from a pregnant mother; a concentration step of concentrating nucleated red blood cells in the maternal blood; a sorting step of sorting the nucleated red blood cells in the maternal blood, in which the nucleated red blood cells are concentrated through the concentration step, into mother-derived nucleated red blood cells and fetus-derived nucleated red blood cells in accordance with the shapes of nuclei and spectral characteristics at wavelengths included in a light wavelength region of 400 to 650 nm; an amplification step of amplifying a nucleic acid of a chromosome of the nucleated red blood cells; a definition step of defining an amount of the amplified product of the nucleated red blood cells; and a determination step of determining presence or absence of a numerical abnormality of the fetus-derived chromosome.