公开(公告)号：US09835631B2

公开(公告)日：2017-12-05

申请号：US15014241

申请日：2016-02-03

申请人： Federica Genovese ,  Mette Juul Nielsen ,  Lise Larsen ,  Diana Julie Oersnes-Leeming ,  Morten Karsdal

发明人： Federica Genovese ,  Mette Juul Nielsen ,  Lise Larsen ,  Diana Julie Oersnes-Leeming ,  Morten Karsdal

IPC分类号： G01N33/53 ,  G01N33/68 ,  G01N33/577

CPC分类号： G01N33/6893 ,  G01N33/577 ,  G01N2333/78 ,  G01N2800/085 ,  G01N2800/52 ,  G01N2800/7052

摘要： Provided herein is a sandwich immunoassay for detecting cross-linked PIIINP that has at least two strands of PIIINP joined together by inter-strand cross-linking each having a C-terminal neo-epitope of PIIINP that is generated by N-protease cleavage of intact type III procollagen. A biological sample having the cross-linked PIIINP is contacted with a first surface-bound monoclonal antibody and then by a second monoclonal antibody, both specifically reactive with a neoepitope in the C-terminal sequence of PIIINP, and then binding of the second monoclonal antibody is determined. Also provided is a method for evaluating the efficacy of an antagonist drug targeting lysyl oxidases via the immunoassay and a kit containing a solid support binding the first monoclonal antibody and containing the second monoclonal antibody.

公开(公告)号：US20160223568A1

公开(公告)日：2016-08-04

申请号：US15014241

申请日：2016-02-03

申请人： Federica Genovese ,  Mette Juul Nielsen ,  Lise Larsen ,  Diana Julie Oersnes-Leeming ,  Morten Karsdal

发明人： Federica Genovese ,  Mette Juul Nielsen ,  Lise Larsen ,  Diana Julie Oersnes-Leeming ,  Morten Karsdal

IPC分类号： G01N33/68 ,  G01N33/577

CPC分类号： G01N33/6893 ,  G01N33/577 ,  G01N2333/78 ,  G01N2800/085 ,  G01N2800/52 ,  G01N2800/7052

摘要： Provided herein is a sandwich immunoassay for detecting cross-linked PIIINP that has at least two strands of PIIINP joined together by inter-strand cross-linking each having a C-terminal neo-epitope of PIIINP that is generated by N-protease cleavage of intact type III procollagen. A biological sample having the cross-linked PIIINP is contacted with a first surface-bound monoclonal antibody and then by a second monoclonal antibody, both specifically reactive with a neoepitope in the C-terminal sequence of PIIINP, and then binding of the second monoclonal antibody is determined. Also provided is a method for evaluating the efficacy of an antagonist drug targeting lysyl oxidases via the immunoassay and a kit containing a solid support binding the first monoclonal antibody and containing the second monoclonal antibody.

摘要翻译： 本文提供了一种夹心免疫测定法，用于检测交联的PIIINP，其具有通过链间交联连接在一起的至少两条PIIINP链，每条链均具有由完整的N-蛋白酶切割产生的PIIINP的C-末端新表位 III型原胶原。 将具有交联的PIIINP的生物样品与第一表面结合的单克隆抗体接触，然后与第二单克隆抗体接触，所述第二单克隆抗体与PIIINP的C末端序列中的新表皮特异性反应，然后与第二单克隆抗体 决心，决意，决定。 还提供了用于通过免疫测定法评估靶向赖氨酰氧化酶的拮抗剂药物的功效的方法，以及含有结合第一单克隆抗体并含有第二单克隆抗体的固体支持物的试剂盒。

公开(公告)号：US09891234B2

公开(公告)日：2018-02-13

申请号：US14116924

申请日：2012-05-04

申请人： Natasha Barascuk Michaelsen ,  Federica Genovese ,  Morten Karsdal ,  Diana Julie Leeming

发明人： Natasha Barascuk Michaelsen ,  Federica Genovese ,  Morten Karsdal ,  Diana Julie Leeming

IPC分类号： G01N33/53 ,  G01N31/00 ,  G01N33/68 ,  C07K16/18 ,  C12Q1/37 ,  C07K14/47

CPC分类号： G01N33/6893 ,  C07K14/4725 ,  C07K16/18 ,  C07K2317/34 ,  C12Q1/37 ,  G01N33/6878 ,  G01N2800/32 ,  G01N2800/323

摘要： A method of bioassay for the quantification of peptide fragments comprising a neo-epitope formed by cleavage of mimecan, a protein of an atherosclerotic plaque, by a proteinase is provided. In the method a sample, such as urine or serum, is contacted with an antibody reactive with the neo-epitope and the level of binding of the antibody to peptide fragments in the sample is determined. The assay is predictive of risk of cardiovascular disease events.