公开(公告)号：US5846802A

公开(公告)日：1998-12-08

申请号：US225488

申请日：1994-04-08

申请人： Frank Buxton ,  Albert Hinnen ,  Jacob Visser

发明人： Frank Buxton ,  Albert Hinnen ,  Jacob Visser

IPC分类号： C12N9/62 ,  C12N15/57 ,  C12N15/80 ,  C12N9/12 ,  C12P19/34

CPC分类号： C12N15/80 ,  C12N9/62

摘要： The present invention concerns a novel DNA sequence coding for an Aspergillus serine protease of the subtilisin-type, an Aspergillus serine protease of the subtilisin-type per se and a method for the preparation thereof. The invention further concerns a novel Aspergillus mutant strain defective in a serine protease of the subtilisin-type, which is useful for the expression of heterologous protein, and a method for the preparation of such a mutant strain.

摘要翻译： 本发明涉及编码枯草杆菌蛋白酶类型的曲霉丝氨酸蛋白酶的新型DNA序列，枯草杆菌蛋白酶型本身的曲霉丝氨酸蛋白酶及其制备方法。 本发明还涉及可用于表达异源蛋白质的枯草杆菌蛋白酶型丝氨酸蛋白酶中缺陷的新型曲霉菌突变菌株，以及这种突变菌株的制备方法。

公开(公告)号：US5756338A

公开(公告)日：1998-05-26

申请号：US731045

申请日：1996-10-08

申请人： Frank Buxton ,  Gabor Jarai ,  Jacob Visser

发明人： Frank Buxton ,  Gabor Jarai ,  Jacob Visser

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N9/62 ,  C12N15/00 ,  C12N15/57 ,  C12N15/58 ,  C12R1/685 ,  C12N15/63 ,  C12N15/80

CPC分类号： C12N9/62

摘要： The present invention concerns a novel DNA sequence coding for an Aspergillus aspartic protease, an Aspergillus aspartic protease per se and a method for the preparation thereof. The invention further concerns a novel Aspergillus mutant strain defective in a protease of the aspartic proteinase-type, which is useful for the expression of heterologous protein, and a method for the preparation of such a mutant strain.

摘要翻译： 本发明涉及编码曲霉天冬氨酸蛋白酶，曲霉天门冬氨酸蛋白酶本身的新型DNA序列及其制备方法。 本发明还涉及可用于异源蛋白质表达的天冬氨酸蛋白酶型蛋白酶中缺陷的新型曲霉菌突变株，以及这种突变菌株的制备方法。

公开(公告)号：US5674728A

公开(公告)日：1997-10-07

申请号：US328314

申请日：1994-10-24

申请人： Frank Buxton ,  Gabor Jarai ,  Jacob Visser

发明人： Frank Buxton ,  Gabor Jarai ,  Jacob Visser

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N9/62 ,  C12N15/00 ,  C12N15/57 ,  C12N15/58 ,  C12R1/685 ,  C12N15/80 ,  C12N15/90

CPC分类号： C12N9/62

摘要： The present invention concerns a novel DNA sequence coding for an Aspergillus aspartic protease, an Aspergillus aspartic protease per se and a method for the preparation thereof. The invention further concerns a novel Aspergillus mutant strain defective in a protease of the aspartic proteinase-type, which is useful for the expression of heterologous protein, and a method for the preparation of such a mutant strain.

摘要翻译： 本发明涉及编码曲霉天冬氨酸蛋白酶，曲霉天门冬氨酸蛋白酶本身的新型DNA序列及其制备方法。 本发明还涉及可用于异源蛋白质表达的天冬氨酸蛋白酶型蛋白酶中缺陷的新型曲霉菌突变株，以及这种突变菌株的制备方法。

公开(公告)号：US5646037A

公开(公告)日：1997-07-08

申请号：US333894

申请日：1994-11-02

申请人： Frank Buxton

发明人： Frank Buxton

IPC分类号： C07K14/65 ,  C07K14/815 ,  C12N1/19 ,  C12N15/09 ,  C12N15/81 ,  C12P21/00 ,  C12R1/19 ,  C12R1/865 ,  C12N1/15 ,  C12N1/21

CPC分类号： C12N15/81 ,  C07K14/65 ,  C07K14/815

摘要： The invention relates to the field of genetic engineering and provides novel hybrid vectors based on the yeast two micron plasmid. The novel hybrid vectors are stably maintained in the cell during proliferation and are useful for the expression of heterologous genes in yeast in large scale batch and particularly in continuous culture.

摘要翻译： 本发明涉及基因工程领域，提供了基于酵母双微质粒的新型杂合载体。 新型杂合载体在增殖期间稳定地保持在细胞中，并且可用于在大规模批次中，特别是在连续培养中在酵母中表达异源基因。