公开(公告)号：US07101548B2

公开(公告)日：2006-09-05

申请号：US09931700

申请日：2001-08-16

申请人： Frank Cuttitta ,  Alfredo Martinez ,  Mae Jean Miller ,  Edward J. Unsworth ,  William Hook ,  Thomas Walsh ,  Karen Gray ,  Charles Macri

发明人： Frank Cuttitta ,  Alfredo Martinez ,  Mae Jean Miller ,  Edward J. Unsworth ,  William Hook ,  Thomas Walsh ,  Karen Gray ,  Charles Macri

IPC分类号： A61K39/395

CPC分类号： C07K16/26 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/505 ,  A61K2039/51 ,  C07K14/575 ,  C07K2317/34 ,  C07K2317/73

摘要： The methods of the present invention demonstrate that adrenomedullin (AM) is expressed in human cancer cell lines of diverse origin and functions as a universal autocrine growth factor driving neoplastic proliferation. The present invention provides for AM peptides and AM antibodies useful in therapeutic, pharmacologic and physiologic compositions. The present invention additionally provides for methods of diagnosis, treatment and prevention of disease utilizing compositions comprising the AM peptides and antibodies of the present invention. The methods of the present invention also provide for experimental models for use in identifying the role of AM in pancreatic physiology. The methods pertaining to rat isolated islets have shown that AM inhibits insulin secretion in a dose-dependent manner. The monoclonal antibody MoAb-G6, which neutralizes AM bioactivity, was shown by the methods of the present invention to increase insulin release fivefold, an effect that was reversed by the addition of synthetic AM.

摘要翻译： 本发明的方法证明，肾上腺髓质素（AM）在不同来源的人类癌细胞系中表达，并且作为驱动肿瘤增殖的通用自分泌生长因子起作用。 本发明提供可用于治疗，药理和生理组合物的AM肽和AM抗体。 本发明另外提供了使用包含本发明的AM肽和抗体的组合物来诊断，治疗和预防疾病的方法。 本发明的方法还提供了用于鉴定AM在胰腺生理学中的作用的实验模型。 与大鼠分离的胰岛相关的方法已经表明AM以剂量依赖的方式抑制胰岛素分泌。 通过本发明的方法显示了中和AM生物活性的单克隆抗体MoAb-G6，以增加胰岛素释放的五倍，这是通过加入合成AM而逆转的作用。

公开(公告)号：US20100021469A1

公开(公告)日：2010-01-28

申请号：US12569821

申请日：2009-09-29

申请人： Frank Cuttitta ,  Alfredo Martinez ,  Mae Jean Miller ,  Edward J. Unsworth ,  William A. Hook ,  Thomas Walsh ,  Karen Gray ,  Charles Macri

发明人： Frank Cuttitta ,  Alfredo Martinez ,  Mae Jean Miller ,  Edward J. Unsworth ,  William A. Hook ,  Thomas Walsh ,  Karen Gray ,  Charles Macri

IPC分类号： A61K39/395 ,  C07K16/00 ,  G01N33/53 ,  A61P7/12 ,  A61P37/00

CPC分类号： C07K16/26 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/505 ,  A61K2039/51 ,  C07K14/575 ,  C07K2317/34 ,  C07K2317/73

摘要： The methods of the present invention demonstrate that adrenomedullin (AM) is expressed in human cancer cell lines of diverse origin and functions as a universal autocrine growth factor driving neoplastic proliferation. The present invention provides for AM peptides and AM antibodies useful in therapeutic, pharmacologic and physiologic compositions. The present invention additionally provides for methods of diagnosis, treatment and prevention of disease utilizing compositions comprising the AM peptides and antibodies of the present invention. The methods of the present invention also provide for experimental models for use in identifying the role of AM in pancreatic physiology. The methods pertaining to rat isolated islets have show that AM inhibits insulin secretion in a dose-dependent manner. The monoclonal antibody MoAb-G6, which neutralizes AM bioactivity, was show by the methods of the present invention to increase insulin release fivefold, an effect that was reversed by the addition of synthetic AM.

摘要翻译： 本发明的方法证明，肾上腺髓质素（AM）在不同来源的人类癌细胞系中表达，并且作为驱动肿瘤增殖的通用自分泌生长因子起作用。 本发明提供可用于治疗，药理和生理组合物的AM肽和AM抗体。 本发明另外提供了使用包含本发明的AM肽和抗体的组合物来诊断，治疗和预防疾病的方法。 本发明的方法还提供了用于鉴定AM在胰腺生理学中的作用的实验模型。 与大鼠分离的胰岛有关的方法表明，AM以剂量依赖的方式抑制胰岛素分泌。 通过本发明的方法显示了中和AM生物活性的单克隆抗体MoAb-G6，以增加胰岛素释放的五倍，这是通过加入合成AM而逆转的作用。

公开(公告)号：US06320022B1

公开(公告)日：2001-11-20

申请号：US09011922

申请日：1998-02-17

申请人： Frank Cutitta ,  Alfredo Martinez ,  Mae Jean Miller ,  Edward J. Unsworth ,  William Hook ,  Thomas Walsh ,  Karen Gray ,  Charles Macri

发明人： Frank Cutitta ,  Alfredo Martinez ,  Mae Jean Miller ,  Edward J. Unsworth ,  William Hook ,  Thomas Walsh ,  Karen Gray ,  Charles Macri

IPC分类号： A61K3800

CPC分类号： C07K16/26 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/505 ,  A61K2039/51 ,  C07K14/575 ,  C07K2317/34 ,  C07K2317/73

摘要： The methods of the present invention demonstrate that adrenomedullin (AM) is expressed in human cancer cell lines of diverse origin and functions as a universal autocrine growth factor driving neoplastic proliferation. The present invention provides for AM peptides and AM antibodies useful in therapeutic, pharmacologic and physiologic compositions. The present invention additionally provides for methods of diagnosis, treatment and prevention of disease utilizing compositions comprising the AM peptides and antibodies of the present invention. The methods of the present invention also provide for experimental models for use in identifying the role of AM in pancreatic physiology. The methods pertaining to rat isolated islets have shown that AM inhibits insulin secretion in a dose-dependent manner. The monoclonal antibody MoAb-G6, which neutralizes AM bioactivity, was shown by the methods of the present invention to increase insulin release fivefold, an effect that was reversed by the addition of synthetic AM.

摘要翻译： 本发明的方法证明，肾上腺髓质素（AM）在不同来源的人类癌细胞系中表达，并且作为驱动肿瘤增殖的通用自分泌生长因子起作用。 本发明提供可用于治疗，药理和生理组合物的AM肽和AM抗体。 本发明另外提供了使用包含本发明的AM肽和抗体的组合物来诊断，治疗和预防疾病的方法。 本发明的方法还提供了用于鉴定AM在胰腺生理学中的作用的实验模型。 与大鼠分离的胰岛相关的方法已经表明AM以剂量依赖的方式抑制胰岛素分泌。 通过本发明的方法显示了中和AM生物活性的单克隆抗体MoAb-G6，以增加胰岛素释放的五倍，这是通过加入合成AM而逆转的作用。

公开(公告)号：US20070004630A1

公开(公告)日：2007-01-04

申请号：US11517599

申请日：2006-09-05

申请人： Frank Cuttitta ,  Alfredo Martinez ,  Mae Miller ,  Edward Unsworth ,  William Hook ,  Thomas Walsh ,  Karen Gray ,  Charles Macri

发明人： Frank Cuttitta ,  Alfredo Martinez ,  Mae Miller ,  Edward Unsworth ,  William Hook ,  Thomas Walsh ,  Karen Gray ,  Charles Macri

IPC分类号： A61K39/395 ,  A61K38/22

CPC分类号： C07K16/26 ,  A61K38/00 ,  A61K39/00 ,  A61K2039/505 ,  A61K2039/51 ,  C07K14/575 ,  C07K2317/34 ,  C07K2317/73

摘要： The methods of the present invention demonstrate that adrenomedullin (AM) is expressed in human cancer cell lines of diverse origin and functions as a universal autocrine growth factor driving neoplastic proliferation. The present invention provides for AM peptides and AM antibodies useful in therapeutic, pharmacologic and physiologic compositions. The present invention additionally provides for methods of diagnosis, treatment and prevention of disease utilizing compositions comprising the AM peptides and antibodies of the present invention. The methods of the present invention also provide for experimental models for use in identifying the role of AM in pancreatic physiology. The methods pertaining to rat isolated islets have show that AM inhibits insulin secretion in a dose-dependent manner. The monoclonal antibody MoAb-G6, which neutralizes AM bioactivity, was show by the methods of the present invention to increase insulin release fivefold, an effect that was reversed by the addition of synthetic AM.

摘要翻译： 本发明的方法证明，肾上腺髓质素（AM）在不同来源的人类癌细胞系中表达，并且作为驱动肿瘤增殖的通用自分泌生长因子起作用。 本发明提供可用于治疗，药理和生理组合物的AM肽和AM抗体。 本发明另外提供了使用包含本发明的AM肽和抗体的组合物来诊断，治疗和预防疾病的方法。 本发明的方法还提供了用于鉴定AM在胰腺生理学中的作用的实验模型。 与大鼠分离的胰岛有关的方法表明，AM以剂量依赖的方式抑制胰岛素分泌。 中和AM生物活性的单克隆抗体MoAb-G6通过本发明的方法显示出增加胰岛素释放的五倍，这是通过加入合成AM而逆转的作用。

公开(公告)号：US20070020278A1

公开(公告)日：2007-01-25

申请号：US11536339

申请日：2006-09-28

申请人： Jeffrey Ross ,  Karen Gray

发明人： Jeffrey Ross ,  Karen Gray

IPC分类号： A61K39/395

CPC分类号： G01N33/57411 ,  C07K16/3069 ,  G01N33/57442 ,  G01N33/57449

摘要： Methods for treating or diagnosing cancers of the female reproductive tract and childhood cancers are disclosed. The methods described herein use binding agents, e.g., antibodies, specific for the extracellular domain of human prostate specific membrane antigen (PSMA).

摘要翻译： 公开了治疗或诊断女性生殖道和儿童期癌症的方法。 本文描述的方法使用对人前列腺特异性膜抗原（PSMA）的细胞外结构域特异的结合剂，例如抗体。

公开(公告)号：US20050156902A1

公开(公告)日：2005-07-21

申请号：US10761572

申请日：2004-01-20

申请人： Roger McPherson ,  Karen Gray ,  James Wood

发明人： Roger McPherson ,  Karen Gray ,  James Wood

IPC分类号： G09G5/00

CPC分类号： G06F1/1601 ,  B60R11/0235 ,  B60R11/0252 ,  B60R11/0264 ,  G06F1/1626

摘要： An electronic viewing tablet is provided with a uni-body display and touch screen system. The device includes a large display, connectors for USB, flash ROM ports (such as Smart Media, Compact Flash and Secure Digital/Multi-Media Flash) protected behind a waterproof access door, a wireless network interface, a momentary contact switch and a waterproof access panel for servicing the internal battery. An inductive charging system may be provided to recharge an internal battery. A microprocessor based electronic communication and control system controls the electronic components of the device. The electronic tablet allows a user to acquire content and applications on a personal computer or network or purchase data and images on removable flash media. Applications, data and images (electronic books, magazines, newspapers, text files, sheet music and “printed” files) may be downloaded to the tablet through a USB connector, a wireless connection, or through a ROM port. Once loaded onto the tablet, written material may be read wherever the user chooses, independent of the computer or network. A user interface presents simple commands to the user, presented in a graphical fashion on a display, allowing the user to navigate the information stored in the device and execute the applications that are present. Soft buttons on the touch screen allow the user to interact with the tablet.

摘要翻译： 电子观看平板电脑配有单体显示屏和触摸屏系统。 该设备包括一个大型显示器，用于USB的连接器，防水门后保护的闪存ROM端口（如智能媒体，紧凑闪存和安全数字/多媒体闪存），无线网络接口，瞬时接触开关和防水 检修面板用于维修内部电池。 可以提供感应充电系统以对内部电池进行再充电。 基于微处理器的电子通信和控制系统控制设备的电子部件。 电子平板电脑允许用户在个人计算机或网络上获取内容和应用，或者在可移动闪存介质上购买数据和图像。 应用程序，数据和图像（电子书，杂志，报纸，文本文件，乐谱和“打印”文件）可以通过USB连接器，无线连接或通过ROM端口下载到平板电脑。 一旦加载到平板电脑上，写入的材料可以在用户选择的任何地方读取，独立于计算机或网络。 用户界面向用户呈现简单命令，以图形方式呈现在显示器上，允许用户导航存储在设备中的信息并执行存在的应用程序。 触摸屏上的软按钮允许用户与平板电脑进行互动。

公开(公告)号：US20050202020A1

公开(公告)日：2005-09-15

申请号：US11027954

申请日：2004-12-30

申请人： Jeffrey Ross ,  Karen Gray

发明人： Jeffrey Ross ,  Karen Gray

IPC分类号： A61K39/395 ,  C07K16/30 ,  G01N33/574

CPC分类号： G01N33/57411 ,  C07K16/3069 ,  G01N33/57442 ,  G01N33/57449

摘要： Methods for treating or diagnosing cancers of the female reproductive tract and childhood cancers are disclosed. The methods described herein use binding agents, e.g., antibodies, specific for the extracellular domain of human prostate specific membrane antigen (PSMA).

摘要翻译： 公开了治疗或诊断女性生殖道和儿童期癌症的方法。 本文描述的方法使用对人前列腺特异性膜抗原（PSMA）的细胞外结构域特异的结合剂，例如抗体。