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    Method to obtain male sterile plants
    4.
    发明授权
    Method to obtain male sterile plants 有权
    获取雄性不育植物的方法

    公开(公告)号：US06344602B1

    公开(公告)日：2002-02-05

    申请号：US09458093

    申请日：1999-12-10

    申请人： Frank Michiels ,  Johan Botterman ,  Marc Cornelissen

    发明人： Frank Michiels ,  Johan Botterman ,  Marc Cornelissen

    IPC分类号： A01H500

    CPC分类号： C12N15/8289

    摘要： A plant having in the nuclear genome of its cells foreign DNA comprising i) a male-sterility gene comprising a male-sterility DNA encoding a sterility RNA, protein or polypeptide which, when produced or overproduced in a stamen cell of the plant, significantly disturbs the metabolism, functioning and/or development of the stamen cell, and, a sterility promoter directing expression of the male-sterility DNA selectively in specific stamen cells of said plant, the male-sterility DNA being in the same transcriptional unit as, and under the control of, the sterility promoter, and ii) a coregulating gene comprising a coregulating DNA encoding a coregulating RNA, protein or polypeptide which, when produced in plant cells wherein said sterility RNA, protein or polypeptide is produced, prevents the activity of said sterility RNA, protein or polypeptide, and a coregulating promoter which is selected from the group consisting of a promoter directing expression of said coregulating DNA in non-stamen cells of said plant, while directing low-level expression in said specific stamen cells; and a promoter comprising a minimal promoter element, whereby said coregulating DNA is in a transcriptional unit which is different from the transcriptional unit of said sterility DNA and wherein said plant is male sterile.

    摘要翻译： 一种在其细胞核基因组中具有外源DNA的植物，其包含i）包含编码无菌RNA，蛋白质或多肽的雄性不育DNA的雄性不育基因，当在植物的雄蕊细胞中产生或过量产生时，其显着干扰 雄蕊细胞的代谢，功能和/或发育，以及在所述植物的特定雄蕊细胞中选择性地引导雄性不育DNA的表达的无菌启动子，雄性不育DNA处于与下述相同的转录单位 无菌启动子的控制，以及ii）包含编码编码RNA，蛋白质或多肽的串联DNA的结合基因，当在产生所述不育RNA，蛋白质或多肽的植物细胞中产生时，其阻止所述不育的活性 RNA，蛋白质或多肽，以及配糖启动子，其选自引导所述结合DNA在非抗体中表达的启动子 所述植物的细胞，同时指导所述特定雄蕊细胞中的低水平表达; 和包含最小启动子元件的启动子，其中所述配糖DNA处于与所述不育DNA的转录单位不同的转录单位中，并且其中所述植物是雄性不育的。

    Method to obtain male sterile plants
    5.
    发明授权
    Method to obtain male sterile plants 失效
    获取雄性不育植物的方法

    公开(公告)号：US6025546A

    公开(公告)日：2000-02-15

    申请号：US894440

    申请日：1997-11-12

    申请人： Frank Michiels ,  Johan Botterman ,  Marc Cornelissen

    发明人： Frank Michiels ,  Johan Botterman ,  Marc Cornelissen

    IPC分类号： A01H5/00 ,  A01H1/02 ,  C12N5/10 ,  C12N15/09 ,  C12N15/55 ,  C12N15/82 ,  C12N15/31

    CPC分类号： C12N15/8289

    摘要： A plant having in the nuclear genome of its cells foreign DNA comprisinga male-sterility gene comprising:a male-sterility DNA encoding a sterility RNA, protein or polypeptide which, when produced or overproduced in a stamen cell of the plant, significantly disturbs the metabolism, functioning and/or development of the stamen cell, and,a sterility promoter directing expression of the male-sterility DNA selectively in specific stamen cells of said plant, the male-sterility DNA being in the same transcriptional unit as, and under the control of, the sterility promoter, anda coregulating gene comprising:a coregulating DNA encoding a coregulating RNA, protein or polypeptide which is capable, when produced in plant cells wherein said sterility RNA, protein or polypeptide is produced, of sufficiently preventing the activity of said sterility RNA, protein or polypeptide,whereby said coregulating DNA is in a transcriptional unit which is different from the transcriptional unit of said sterility DNA.

    摘要翻译： PCT No.PCT / EP96 / 00722 Sec。 371日期：1997年11月12日 102（e）日期1997年11月12日PCT 1996年2月21日PCT PCT。 WO96 / 26283 PCT出版物 日期1996年8月29日具有核细胞核基因组的植物，其外源DNA包含雄性不育基因，其包含：编码不育RNA，蛋白质或多肽的雄性不育DNA，其在产生或过量产生于雄性不育基因的雄蕊细胞中时 植物，显着地扰乱雄蕊细胞的代谢，功能和/或发育，以及在所述植物的特定雄蕊细胞中选择性地引导雄性不育DNA的表达的无菌启动子，雄性不育DNA处于相同的转录单位 作为和控制下的无菌启动子，以及调控基因，其包含：编码编码RNA，蛋白质或多肽的串联DNA，其能够在其中产生所述不育性RNA，蛋白质或多肽的植物细胞中产生时 充分防止所述无菌RNA，蛋白质或多肽的活性，由此所述结合DNA处于与转录不同的转录单位 l所述不育DNA单位。