公开(公告)号：US07551271B2

公开(公告)日：2009-06-23

申请号：US11893012

申请日：2007-08-13

申请人： Frank Witney ,  Gary McMaster ,  Quan Nguyen ,  Steve Chen

发明人： Frank Witney ,  Gary McMaster ,  Quan Nguyen ,  Steve Chen

IPC分类号： G01J1/00

CPC分类号： G01N21/6452

摘要： Uncaging devices that can be used to uncage photoactivatable caged components are provided. Consistent, uniform and/or high throughput processing of reactions and assays that include caged components is provided. Masked multiwell plates that can be used for uncaging photoactivatable caged components are provided. Methods and apparatus for initiating assays involving caged components are provided.

摘要翻译： 提供了可用于解除可光灭活的笼式组件的非屏蔽设备。 提供了包括笼式组件的反应和测定的一致，均匀和/或高通量处理。 提供可用于解码可光致灭活笼式组件的蒙版多孔板。 提供了用于启动包含笼式组件的测定的方法和装置。

公开(公告)号：US07271886B2

公开(公告)日：2007-09-18

申请号：US10716176

申请日：2003-11-17

申请人： Frank Witney ,  Gary McMaster ,  Quan Nguyen ,  Steve Chen

发明人： Frank Witney ,  Gary McMaster ,  Quan Nguyen ,  Steve Chen

IPC分类号： G01J1/00

CPC分类号： G01N21/6452

摘要： Uncaging devices that can be used to uncage photoactivatable caged components are provided. Consistent, uniform and/or high throughput processing of reactions and assays that include caged components is provided. Masked multiwell plates that can be used for uncaging photoactivatable caged components are provided. Methods and apparatus for initiating assays involving caged components are provided.

摘要翻译： 提供了可用于解除可光灭活的笼式组件的非屏蔽设备。 提供了包括笼式组件的反应和测定的一致，均匀和/或高通量处理。 提供了可用于解码可光致灭活笼式组件的蒙版多孔板。 提供了用于启动包含笼式组件的测定的方法和装置。

公开(公告)号：US20050051706A1

公开(公告)日：2005-03-10

申请号：US10716176

申请日：2003-11-17

申请人： Frank Witney ,  Gary McMaster ,  Quan Nguyen ,  Steve Chen

发明人： Frank Witney ,  Gary McMaster ,  Quan Nguyen ,  Steve Chen

IPC分类号： A61K48/00 ,  C07H21/02 ,  C12Q1/68 ,  G01J1/00 ,  G01J1/42 ,  G01J3/30 ,  G01N20060101 ,  H01J40/14

CPC分类号： G01N21/6452

摘要： Uncaging devices that can be used to uncage photoactivatable caged components are provided. Consistent, uniform and/or high throughput processing of reactions and assays that include caged components is provided. Masked multiwell plates that can be used for uncaging photoactivatable caged components are provided. Methods and apparatus for initiating assays involving caged components are provided.

摘要翻译： 提供了可用于解除可光灭活的笼式组件的非屏蔽设备。 提供了包括笼式组件的反应和测定的一致，均匀和/或高通量处理。 提供了可用于解码可光致灭活笼式组件的蒙版多孔板。 提供了用于启动包含笼式组件的测定的方法和装置。

公开(公告)号：US20080030713A1

公开(公告)日：2008-02-07

申请号：US11893012

申请日：2007-08-13

申请人： Frank Witney ,  Gary McMaster ,  Quan Nguyen ,  Steve Chen

发明人： Frank Witney ,  Gary McMaster ,  Quan Nguyen ,  Steve Chen

IPC分类号： G01J3/00 ,  G01J1/00

CPC分类号： G01N21/6452

摘要： Uncaging devices that can be used to uncage photoactivatable caged components are provided. Consistent, uniform and/or high throughput processing of reactions and assays that include caged components is provided. Masked multiwell plates that can be used for uncaging photoactivatable caged components are provided. Methods and apparatus for initiating assays involving caged components are provided.

摘要翻译： 提供了可用于解除可光灭活的笼式组件的非屏蔽设备。 提供了包括笼式组件的反应和测定的一致，均匀和/或高通量处理。 提供可用于解码可光致灭活笼式组件的蒙版多孔板。 提供了用于启动包含笼式组件的测定的方法和装置。

公开(公告)号：US20050282203A1

公开(公告)日：2005-12-22

申请号：US11134851

申请日：2005-05-17

申请人： Quan Nguyen ,  Gary McMaster ,  Frank Witney

发明人： Quan Nguyen ,  Gary McMaster ,  Frank Witney

IPC分类号： A61K48/00 ,  C07H21/02 ,  C12Q1/68 ,  G01J1/00 ,  G01J1/42 ,  G01J3/30 ,  G01N20060101 ,  H01J40/14

CPC分类号： G01N21/6452

摘要： Methods of using labeled interfering RNAs to detect and/or quantitate target mRNAs in cells are provided. Related compositions, systems, and kits are also provided. Caged interfering RNAs (e.g., photoactivatable interfering RNAs), methods of using such caged RNAs, and related systems and kits are also provided. Caged RNAs capable of repressing translation of a target mRNA or silencing transcription of a target gene are also provided, along with related methods, systems, and kits. Methods and compositions for introducing RNAs into cells, using RNAs covalently associated with protein transduction domains and/or lipids, are provided. Also provided are methods and compositions for selectively attenuating expression of a target mRNA by controlling expression of an interfering RNA, an RNA capable of initiating translation repression, or an RNA capable of initiating transcriptional silencing.

摘要翻译： 提供了使用标记的干扰RNA来检测和/或定量细胞中靶mRNA的方法。 还提供了相关的组合物，系统和试剂盒。 还提供了笼式干扰RNA（例如可光活化干扰RNA），使用这种笼式RNA的方法以及相关系统和试剂盒。 还提供能够抑制靶mRNA翻译或沉默靶基因转录的笼状RNA以及相关方法，系统和试剂盒。 提供了使用与蛋白质转导结构域和/或脂质共价缔合的RNA将RNA引入细胞的方法和组合物。 还提供了通过控制干扰RNA的表达，能够启动翻译抑制的RNA或能够启动转录沉默的RNA来选择性减弱靶mRNA表达的方法和组合物。

公开(公告)号：US07541193B2

公开(公告)日：2009-06-02

申请号：US10716174

申请日：2003-11-17

申请人： Quan Nguyen ,  Gary McMaster

发明人： Quan Nguyen ,  Gary McMaster

IPC分类号： G01N21/76

CPC分类号： C12Q1/485 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q1/37 ,  C12Q1/6818 ,  G01N33/542 ,  G01N33/573 ,  C12Q2537/1373 ,  C12Q2525/207

摘要： A number of caged (e.g., photoactivatable) compositions are provided, for example, caged enzyme sensors, caged binding sensors, caged nucleic acid probes, caged interfering RNAs, caged antisense nucleic acids, caged ribozymes, caged biomolecular analogs, caged transcription factors, caged antibodies, caged molecular decoys, and caged aptamers. Compositions comprising a plurality of caged components are also provided, as are labeled and optionally caged modulators. Also provided are kits for making the caged compositions and methods for making and for using the caged compositions. Related systems and devices are also provided (e.g., an uncaging device that can trigger photoactivatable assays and optionally detect signals from the assays).

摘要翻译： 提供了许多笼状（例如，可光活化的）组合物，例如笼式酶传感器，笼式结合传感器，笼式核酸探针，笼式干扰RNA，笼式反义核酸，笼式核酶，笼式生物分子类似物，笼状转录因子，笼养 抗体，笼状分子诱饵和笼状适体。 还提供了包括多个笼状组分的组合物，如标记的和任选的笼式调节剂。 还提供了用于制备笼形组合物和制备和使用笼形组合物的方法的试剂盒。 还提供了相关系统和装置（例如，能够触发可光活化测定并且可选地检测来自测定的信号的解开装置）。

公开(公告)号：US20050059028A1

公开(公告)日：2005-03-17

申请号：US10716393

申请日：2003-11-17

申请人： Quan Nguyen ,  Gary McMaster

发明人： Quan Nguyen ,  Gary McMaster

IPC分类号： A61K48/00 ,  C07H21/02 ,  C12N15/85 ,  C12Q1/00 ,  C12Q1/37 ,  C12Q1/48 ,  C12Q1/68 ,  G01N33/542 ,  G01N33/573

CPC分类号： C12Q1/485 ,  B82Y5/00 ,  B82Y10/00 ,  C12Q1/37 ,  C12Q1/6818 ,  G01N33/542 ,  G01N33/573 ,  C12Q2537/1373 ,  C12Q2525/207

摘要： Methods of using labeled interfering RNAs to detect and/or quantitate target mRNAs in cells are provided. Related compositions, systems, and kits are also provided. Caged interfering RNAs (e.g., photoactivatable interfering RNAs), methods of using such caged RNAs, and related systems and kits are also provided. Methods and compositions for introducing interfering RNAs into cells, using RNAs covalently associated with protein transduction domains and/or lipids, are provided. Also provided are methods and compositions for selectively attenuating expression of a target mRNA by controlling expression of an interfering RNA.

摘要翻译： 提供了使用标记的干扰RNA来检测和/或定量细胞中靶mRNA的方法。 还提供了相关的组合物，系统和试剂盒。 还提供了笼式干扰RNA（例如可光活化干扰RNA），使用这种笼式RNA的方法以及相关系统和试剂盒。 提供了使用与蛋白质转导结构域和/或脂质共价缔合的RNA将干扰RNA引入细胞的方法和组合物。 还提供了通过控制干扰RNA的表达来选择性地减弱靶mRNA表达的方法和组合物。

公开(公告)号：US20120240757A1

公开(公告)日：2012-09-27

申请号：US13415464

申请日：2012-03-08

申请人： David Arthur Schade ,  Torrey Pike ,  George Thomas ,  Quan Nguyen ,  Mike Iwen

发明人： David Arthur Schade ,  Torrey Pike ,  George Thomas ,  Quan Nguyen ,  Mike Iwen

IPC分类号： F41H5/02 ,  F41H7/00

CPC分类号： F41H5/026

摘要： A grille having a plurality of s-louvers shaped to increase the efficiency of air flow through the grille without decreasing the effectiveness of the louvers at stopping or deflecting projectiles. Each louver has a hooked portion at the end of the louver to present a ballistic hook for stopping projectiles ricocheting through the circuitous path defined between the louvers. An insert having a closeout for covering the ballistic hook is positioned within each hooked shape portion to eliminate the eddy or stall created at the end of the circuitous path by ballistic hook. The closeout can be penetrated by projectiles ricocheting through the circuitous path such that the ballistic hook can still capture projectiles within the inlet.

摘要翻译： 具有多个s形百叶窗的格栅，其形状以提高通过格栅的空气流动的效率，而不会降低百叶窗在停止或偏转抛射体时的有效性。 每个百叶窗在百叶窗的末端具有钩状部分，以呈现弹道钩，用于通过限定在百叶窗之间的迂回路径来阻止弹丸的跳动。 具有用于覆盖防弹钩的封闭件的插入件位于每个钩形形状部分内，以消除由弹道钩在迂回路径的末端产生的涡流或失速。 可以通过迂回通道的射弹穿透关闭，使得弹道钩仍能捕获入口内的射弹。

公开(公告)号：US20070280022A1

公开(公告)日：2007-12-06

申请号：US11421497

申请日：2006-06-01

申请人： Lam Van Nguyen ,  Quan Nguyen

发明人： Lam Van Nguyen ,  Quan Nguyen

IPC分类号： G11C7/02

CPC分类号： G11C7/14 ,  G11C7/12 ,  G11C7/227 ,  G11C11/412 ,  G11C11/419

摘要： A dummy SRAM cell for use in a dummy bit line circuit uses the same transistors as used in a standard SRAM cell, which includes first and second subsets of transistors configured as first and second bit line output circuits. The dummy SRAM cell includes the same first and second subsets of transistors, with the first transistors configured as a dummy bit line output circuit having substantially the same electrical characteristics as the first bit line output circuit of the standard SRAM cell. Further, the second transistors, which are not otherwise needed for the dummy SRAM cell function, are reconfigured as a voltage tie circuit for the dummy bit line output. Using the second transistors for this purpose obviates the need to add additional transistors to form a voltage tie circuit for configuring the dummy bit line output circuit as a load or driver for the dummy bit line.

摘要翻译： 用于虚拟位线电路的虚拟SRAM单元使用与标准SRAM单元中使用的相同的晶体管，其包括配置为第一和第二位线输出电路的晶体管的第一和第二子集。 虚拟SRAM单元包括相同的第一和第二晶体管子集，其中第一晶体管配置为具有与标准SRAM单元的第一位线输出电路基本相同的电特性的虚拟位线输出电路。 此外，虚拟SRAM单元功能不另外需要的第二晶体管被重新配置为虚拟位线输出的电压连接电路。 为此，使用第二晶体管避免了增加额外的晶体管以形成用于将虚拟位线输出电路配置为虚拟位线的负载或驱动器的电压连接电路的需要。

公开(公告)号：US20050077092A1

公开(公告)日：2005-04-14

申请号：US10923653

申请日：2004-08-20

申请人： Zhou Yong ,  Jim Minikus ,  Amardeep Singh ,  Quan Nguyen ,  Sharath Kolachalam

发明人： Zhou Yong ,  Jim Minikus ,  Amardeep Singh ,  Quan Nguyen ,  Sharath Kolachalam

IPC分类号： E21B10/16 ,  E21B10/50 ,  E21B10/00

CPC分类号： E21B10/50 ,  E21B10/16

摘要： Disclosed are a variety of arcuate-shaped inserts for drill bits, and in particular, for placement in rolling cone cutters of drill bits. The arcuate inserts include 360° or ring-shaped inserts, as well as inserts of smaller arcuate length. The arcuate inserts are suitable for use in all surfaces of the rolling cone cutter, and in other locations in drill bits, and may have specialized cutting surfaces and material enhancements to enhance their cutting duty performance. Certain arcuate inserts may include stress relieving discontinuities such that, upon assembly into the cone or during drilling, the arcuate inserts may fragment in a controlled and predicted manner into shorter arcuate lengths.

摘要翻译： 公开了用于钻头的各种弧形插入件，特别地，用于放置在钻头的滚动锥形切割器中。 弧形刀片包括360°或环形刀片，以及较小弓形长度的刀片。 弧形插入件适用于滚动圆锥切割机的所有表面，以及钻头中的其他位置，并且可具有专门的切割表面和材料增强功能，以增强其切割性能。 某些弓形刀片可包括应力消除不连续性，使得在组装到锥体中或在钻孔期间，弓形刀片可以以受控和预测的方式分段成较短的弓形长度。