IN-PROCESS CONTROL IN A METHOD FOR PRODUCING EPO
    1.
    发明申请
    IN-PROCESS CONTROL IN A METHOD FOR PRODUCING EPO 审中-公开
    生产EPO的方法中的过程控制

    公开(公告)号:US20120021441A1

    公开(公告)日:2012-01-26

    申请号:US13140190

    申请日:2009-12-07

    IPC分类号: G01N33/74 G01N21/78

    CPC分类号: G01N33/746

    摘要: The invention relates to a method for determining the isoform composition of erythropoietin, comprising the following steps: a) isoelectrical focusing of a sample comprising erythropoietin in a gel over a pH range having a lower limit of 2.5 to 3.5 and an upper limit of 5 to 8, wherein the sample comprising erythropoietin originates from a culture supernatant of erythropoietin producing eukaryotic cells; b) transferring of the proteins comprised and separated in the gel to a membrane; c) verifying the erythropoietin bound to the membrane by specific antibodies; and to a method for in-process control of culture supernatants of erythropoietin producing eukaryotic cells during the fermentative production process.

    摘要翻译: 本发明涉及一种测定红细胞生成素的同种型组成的方法,包括以下步骤:a)将包含促红细胞生成素的样品在凝胶中的等电聚焦在pH范围内,其下限为2.5至3.5,上限为5至 8,其中包含促红细胞生成素的样品源自产生促红细胞生成素的真核细胞的培养上清液; b)将在凝胶中包含和分离的蛋白质转移到膜上; c)通过特异性抗体验证与该膜结合的促红细胞生成素; 以及在发酵生产过程中用于在产生促红细胞生成素的真核细胞的培养物上清液中进行过程控制的方法。

    Thinning of granular starch
    4.
    发明授权
    Thinning of granular starch 失效
    颗粒淀粉变薄

    公开(公告)号:US5362868A

    公开(公告)日:1994-11-08

    申请号:US77914

    申请日:1993-06-18

    CPC分类号: C08B31/18

    摘要: A process is disclosed for thinning granular starch in an aqueous slurry employing a source of peracid without using metal catalysts. The thinning process is aimed at modifying granular starches by lowering viscosity/increasing alkaline fluidity. It can also be employed immediately before or during cooking (gelatinization) of starch. Sources of peracids are peroxomonosulfuric acid (Caro's acid) or organic peracids such as performic, peracetic or perpropionic acid. Reaction time and temperature are lower than for existing process while product properties are improved.

    摘要翻译: 公开了一种在不使用金属催化剂的情况下使用过酸源来稀释含水浆料中的颗粒淀粉的方法。 减薄方法的目的是通过降低粘度/增加碱性流动性来改性颗粒状淀粉。 它也可以在淀粉的烹饪之前或期间(糊化)进行使用。 过酸的来源是过碘酸(卡罗酸)或有机过酸,如过氧乙酸或过丙酸。 反应时间和温度低于现有工艺,而产品性能得到改善。

    Destruction of cyanide and other pollutants in aqueous solutions
    5.
    发明授权
    Destruction of cyanide and other pollutants in aqueous solutions 失效
    氰化物和其他污染物在水溶液中的破坏

    公开(公告)号:US5246598A

    公开(公告)日:1993-09-21

    申请号:US993593

    申请日:1992-12-22

    IPC分类号: C02F1/72 C02F9/00

    摘要: A method is disclosed for detoxifying an aqueous waste solution containing cyanide. The method involves adding a source of silicon oxide to the cyanide containing aqueous waste solution in a concentration of 0.001 to 10 g silicon dioxide/liter aqueous waste solution, at an alkaline pH and a temperature of from 10.degree. to 100.degree. C. Hydrogen peroxide or another peroxygen compound is added in a sufficient quantity to convert the aqueous waste solution containing cyanide to an aqueous solution containing environmentally less toxic materials.

    摘要翻译: 公开了一种用于对含氰化物的废水进行解毒的方法。 该方法包括在碱性pH和10至100℃的温度下,将0.001至10g二氧化硅/升废水溶液的浓度添加到含氰化物的废水溶液中。过氧化氢 或者以足够的量加入另外的过氧化合物以将含有氰化物的废水溶液转化为含有环境较少毒性物质的水溶液。