公开(公告)号：US20110003970A1

公开(公告)日：2011-01-06

申请号：US12676961

申请日：2008-09-02

申请人： Frederik Anton De Wolf ,  Martinus Abraham Cohen Stuart ,  Gerrit Eggink ,  Marc Willem Theodoor Werten ,  Aernout Anders Martens

发明人： Frederik Anton De Wolf ,  Martinus Abraham Cohen Stuart ,  Gerrit Eggink ,  Marc Willem Theodoor Werten ,  Aernout Anders Martens

IPC分类号： C07K14/78

CPC分类号： C07K14/78 ,  C07K14/43586 ,  C07K2319/00

摘要： A multi-block copolymer comprises at least one hydrophilic collagen-like block and at least one silk-like block. The silk-like block comprises an amino acid sequence ((GA)mGX)n (SEQ ID NO: 10), wherein G is glycine, A is alanine, m and n are at least 2. X is an ionizable amino acid. The ionizable amino acid can be inducedle to bear a positive charge or a negative charge. The ionizable amino acid bearing the positive charge is preferably histidine and the one bearing the negative charge is preferably glutamic acid.

摘要翻译： 多嵌段共聚物包含至少一种亲水性胶原样嵌段和至少一种丝状嵌段。 丝状块包含氨基酸序列（（GA）mGX）n（SEQ ID NO：10），其中G是甘氨酸，A是丙氨酸，m和n至少为2.X是可电离的氨基酸。 可电离的氨基酸可以被诱导携带正电荷或负电荷。 带有正电荷的可电离氨基酸优选为组氨酸，带负电荷的氨基酸优选为谷氨酸。

公开(公告)号：US08084579B2

公开(公告)日：2011-12-27

申请号：US10342331

申请日：2003-01-15

申请人： George Valentino Van Heerde ,  Alexis Comelus Van Rijn ,  Jan Bastiaan Bouwstra ,  Frederik Anton De Wolf ,  Andreas Mooibroek ,  Marc Willem Theodoor Werten ,  Richèle Deodata Wind ,  Tanja Jacoba Van Den Bosch

发明人： George Valentino Van Heerde ,  Alexis Comelus Van Rijn ,  Jan Bastiaan Bouwstra ,  Frederik Anton De Wolf ,  Andreas Mooibroek ,  Marc Willem Theodoor Werten ,  Richèle Deodata Wind ,  Tanja Jacoba Van Den Bosch

IPC分类号： A61K38/17 ,  A61K38/00

CPC分类号： C07K14/78 ,  G03C1/0051 ,  G03C1/047

摘要： A composition comprising a purified collagen-like polypeptide suitable as a peptizer, said polypeptide comprising [Gly-X-Y] repeats, wherein Gly stands for glycine, X and Y represent any amino acid and n is selected such that the length of the [Gly-X-Y] repeat is at least 2.5 kDa and wherein the amino acid sequence of said polypeptide comprises more than 4 different amino acids and wherein said purified polypeptide is free of helix structure.

摘要翻译： 一种包含适合作为胶溶剂的纯化的胶原样多肽的组合物，所述多肽包含[Gly-XY]重复序列，其中Gly代表甘氨酸，X和Y表示任何氨基酸，n选择为使[Gly- XY]重复是至少2.5kDa，并且其中所述多肽的氨基酸序列包含多于4个不同的氨基酸，并且其中所述纯化的多肽不含螺旋结构。

公开(公告)号：US20090264625A1

公开(公告)日：2009-10-22

申请号：US11802834

申请日：2007-05-25

申请人： George Valentino Van Heerde ,  Alexis Comelus Van Rijn ,  Jan Bastiaan Bouwstra ,  Frederik Anton De Wolf ,  Andreas Mooibroek ,  Marc Willem Theodoor Werten ,  Richele Deodata Wind ,  Tanja Jacoba Van Den Bosch

发明人： George Valentino Van Heerde ,  Alexis Comelus Van Rijn ,  Jan Bastiaan Bouwstra ,  Frederik Anton De Wolf ,  Andreas Mooibroek ,  Marc Willem Theodoor Werten ,  Richele Deodata Wind ,  Tanja Jacoba Van Den Bosch

IPC分类号： C07K14/78 ,  C12P21/00

CPC分类号： C07K14/78 ,  G03C1/0051 ,  G03C1/047

摘要： A composition comprising a purified collagen-like polypeptide suitable as a peptizer, said polypeptide comprising [Gly-X-Y]n repeats, wherein Gly stands for glycine, X and Y represent any amino acid and n is an integer and selected such that the length of the [Gly-X-Y]n repeat is at least 2.5 kDa and wherein the amino acid sequence of said [Gly-X-Y]n repeats comprises more than 4 different amino acids and wherein said purified polypeptide is free of helix structure.

摘要翻译： 包含适合作为胶溶剂的纯化的胶原样多肽的组合物，所述多肽包含[Gly-XY] n重复序列，其中Gly表示甘氨酸，X和Y表示任何氨基酸，n是整数， [Gly-XY] n重复是至少2.5kDa，并且其中所述[Gly-XY] n重复序列的氨基酸序列包含多于4个不同的氨基酸，并且其中所述纯化的多肽不含螺旋结构。

公开(公告)号：US08188230B2

公开(公告)日：2012-05-29

申请号：US11802834

申请日：2007-05-25

申请人： George Valentino Van Heerde ,  Alexis Comelus Van Rijn ,  Jan Bastiaan Bouwstra ,  Frederik Anton De Wolf ,  Andreas Mooibroek ,  Marc Willem Theodoor Werten ,  Richèle Deodata Wind ,  Tanja Jacoba Van Den Bosch

发明人： George Valentino Van Heerde ,  Alexis Comelus Van Rijn ,  Jan Bastiaan Bouwstra ,  Frederik Anton De Wolf ,  Andreas Mooibroek ,  Marc Willem Theodoor Werten ,  Richèle Deodata Wind ,  Tanja Jacoba Van Den Bosch

IPC分类号： A61K38/17 ,  A61K38/00

CPC分类号： C07K14/78 ,  G03C1/0051 ,  G03C1/047

摘要： A composition comprising a purified collagen-like polypeptide suitable as a peptizer, said polypeptide comprising [Gly-X-Y]n repeats, wherein Gly stands for glycine, X and Y represent any amino acid and n is an integer and selected such that the length of the [Gly-X-Y]n repeat is at least 2.5 kDa and wherein the amino acid sequence of said [Gly-X-Y]n repeats comprises more than 4 different amino acids and wherein said purified polypeptide is free of helix structure.

摘要翻译： 包含适合作为胶溶剂的纯化的胶原样多肽的组合物，所述多肽包含[Gly-XY] n重复序列，其中Gly表示甘氨酸，X和Y表示任何氨基酸，n是整数， [Gly-XY] n重复是至少2.5kDa，并且其中所述[Gly-XY] n重复序列的氨基酸序列包含多于4个不同的氨基酸，并且其中所述纯化的多肽不含螺旋结构。

公开(公告)号：US06645712B1

公开(公告)日：2003-11-11

申请号：US09602459

申请日：2000-06-23

申请人： Joseph Hubertus Olijve ,  Jan Bastiaan Bouwstra ,  Frederik Anton De Wolf ,  Marc Willem Theodoor Werten ,  Hendrik Wouter Wisselink ,  Richéle Deodata Wind ,  Tanja Jacoba Van Den Bosch ,  Yuzo Toda

发明人： Joseph Hubertus Olijve ,  Jan Bastiaan Bouwstra ,  Frederik Anton De Wolf ,  Marc Willem Theodoor Werten ,  Hendrik Wouter Wisselink ,  Richéle Deodata Wind ,  Tanja Jacoba Van Den Bosch ,  Yuzo Toda

IPC分类号： G03C108

CPC分类号： G03C1/005

摘要： The invention provides oil-in-water emulsions comprising recombinant collagen-like polymer in an amount sufficient to act as stabiliser of the emulsion. The polymer is especially a polypeptide which is free of helix structure, has an isoelectric point at least 0.5 pH units removed from the pH of the oil-in-water emulsion. Furthermore, amphiphilic recombinant collagen-like polymers are provided for use in oil-in-water emulsions. The amphiphilic polymers are polar at one end as a result of a relative abundance of polar amino acids, and apolar at the other end as a result of a relative abundance of apolar amino acids,

摘要翻译： 本发明提供了包含足以作为乳液稳定剂的量的重组胶原样聚合物的水包油乳液。 聚合物特别是不含螺旋结构的多肽，具有从水包油乳液的pH去除至少0.5个pH单位的等电点。 此外，提供两亲重组胶原样聚合物用于水包油乳剂中。 由于极性氨基酸的相对丰度，两亲聚合物在一端是极性的，另一端由于非极性氨基酸的相对丰度而非极性，

公开(公告)号：US06150081A

公开(公告)日：2000-11-21

申请号：US219849

申请日：1998-12-23

申请人： George Valentino Van Heerde ,  Alexis Cornelus Van Rijn ,  Jan Bastiaan Bouwstra ,  Frederik Anton De Wolf ,  Andreas Mooibroek ,  Marc Willem Theodoor Werten ,  Richele Deodata Wind ,  Tanja Jacoba Van Den Bosch

发明人： George Valentino Van Heerde ,  Alexis Cornelus Van Rijn ,  Jan Bastiaan Bouwstra ,  Frederik Anton De Wolf ,  Andreas Mooibroek ,  Marc Willem Theodoor Werten ,  Richele Deodata Wind ,  Tanja Jacoba Van Den Bosch

IPC分类号： C12N15/09 ,  A61K38/17 ,  C07K5/04 ,  C07K14/78 ,  C12N1/19 ,  C12N1/21 ,  C12R1/66 ,  C12R1/78 ,  C12R1/84 ,  C12R1/885 ,  G03C1/005 ,  G03C1/015 ,  G03C1/035 ,  G03C1/047

CPC分类号： C07K14/78 ,  G03C1/0051 ,  G03C1/047

摘要： A tabular silver halide emulsion wherein the tabular grains account for more than 75% of the total grain projected area said emulsion comprising silver halide grains nucleated in the presence of nucleation peptizer and thereafter grown in the presence of growth peptizer, wherein at least one of the peptizers is substantially pure collagen like material prepared by genetic engineering, said peptizer having an amino acid sequence comprising more than 4 different amino acids. A process of preparing the AgX emulsion. A process of producing recombinant collagen like polypeptide comprising expression of a collagen like polypeptide encoding nucleic acid sequence by a microorganism to a degree exceeding 0.95 grammes/liter, said recombinant collagen being free of helix structure and preferably the expression occurring in a microorganism other than E. coli or Saccharomyces cerevisiae.

摘要翻译： 一种片状卤化银乳剂，其中片状颗粒占总颗粒投影面积的75％以上，所述乳状液包含在成核胶溶剂存在下成核的卤化银颗粒，然后在生长胶溶剂存在下生长，其中至少一种 胶溶剂是通过基因工程制备的基本上纯的胶原蛋白，所述胶溶剂具有包含多于4种不同氨基酸的氨基酸序列。 制备AgX乳液的方法。 一种生产重组胶原蛋白样多肽的方法，包括将微生物编码核酸序列的胶原蛋白多肽表达至超过0.95克/升的程度，所述重组胶原蛋白不含螺旋结构，优选在除E以外的微生物中发生的表达 大肠杆菌或酿酒酵母（Saccharomyces cerevisiae）。