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公开(公告)号：US10704071B2
公开(公告)日：2020-07-07
申请号：US15994950
申请日：2018-05-31
Applicant: Genentech, Inc.
Inventor： Meixia Zhou , Bradley Richard Snedecor , Chi Kin Domingos Ng , Amy Shen
IPC: C12P21/00 , C12N15/113 , C12N1/38 , C12N15/11 , C12N15/85
Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.
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公开(公告)号：US12129480B2
公开(公告)日：2024-10-29
申请号：US16956942
申请日：2018-12-21
Applicant: GENENTECH, INC.
Inventor： Chi Kin Domingos Ng , Yongping Guo Crawford , Amy Shen , Meixia Zhou , Bradley R. Snedecor , Shahram Misaghi , Albert Eric Gao
CPC classification number: C12N15/907 , C07K16/00 , C12N15/90 , C07K2317/51 , C07K2317/515 , C12N2800/30 , C12N2800/90 , C12N2840/00
Abstract: The presently disclosed subject matter relates to targeted integration (TI) host cells suitable for the expression of recombinant proteins, as well as methods of producing and using said TI host cells.
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公开(公告)号：US20240409615A1
公开(公告)日：2024-12-12
申请号：US18752501
申请日：2024-06-24
Applicant: Genentech, Inc.
Inventor： Chi Kin Domingos Ng , Amy Shen , Gavin Christian Barnard
Abstract: The presently disclosed subject matter relates to multi-vector recombinase mediated cassette exchange approaches to achieve targeted integration of sequences of interest for the generation host cells expressing recombinant proteins, e.g., monoclonal antibodies, as well as compositions derived from the same, e.g., bispecific antibodies, and other complex format proteins, e.g., membrane protein complexes, and other difficult to express molecules.
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Patent Agency Ranking
4.发明授权Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase 有权通过下调乳酸脱氢酶和丙酮酸脱氢酶激酶的表达，降低乳酸水平和增加多肽产生公开(公告)号：US09487809B2
公开(公告)日：2016-11-08
申请号：US13688075
申请日：2012-11-28
Applicant: Genentech, Inc.
Inventor： Meixia Zhou , Bradley Richard Snedecor , Chi Kin Domingos Ng , Amy Shen
CPC classification number: C12P21/00 , C12N1/38 , C12N15/111 , C12N15/113 , C12N15/1137 , C12N15/85 , C12N2310/14 , C12N2320/00 , C12Y101/01027 , C12Y102/04001
Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.
Abstract translation: 本发明提供用于降低培养细胞中乳酸盐产生和增加多肽产生的方法和组合物。 一方面，本发明提供一种方法，其包括培养表达a）对乳酸脱氢酶（LDH）特异性的小干扰RNA（siRNA）的细胞和b）对丙酮酸脱氢酶激酶（PDHK）特异的siRNA。 在另一方面，本发明提供培养的细胞或载体，其包含对LDH特异性的siRNA和对PDHK特异性的siRNA。
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5.发明申请DECREASING LACTATE LEVEL AND INCREASING POLYPEPTIDE PRODUCTION BY DOWNREGULATING THE EXPRESSION OF LACTATE DEHYDROGENASE AND PYRUVATE DEHYDROGENASE KINASE 审中-公开通过下调LACTATE DEHYDROGENASE和PYRUVATE DEHYDROGENASE KINASE的表达来降低LACTATE水平并增加多肽生产公开(公告)号：US20130084605A1
公开(公告)日：2013-04-04
申请号：US13688075
申请日：2012-11-28
Applicant: Genentech, Inc.
Inventor： Meixia Zhou , Bradley Richard Snedecor , Chi Kin Domingos Ng , Amy Shen
CPC classification number: C12P21/00 , C12N1/38 , C12N15/111 , C12N15/113 , C12N15/1137 , C12N15/85 , C12N2310/14 , C12N2320/00 , C12Y101/01027 , C12Y102/04001
Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.
Abstract translation: 本发明提供用于降低培养细胞中乳酸盐产生和增加多肽产生的方法和组合物。 一方面，本发明提供一种方法，其包括培养表达a）对乳酸脱氢酶（LDH）特异性的小干扰RNA（siRNA）的细胞和b）对丙酮酸脱氢酶激酶（PDHK）特异的siRNA。 在另一方面，本发明提供培养的细胞或载体，其包含对LDH特异性的siRNA和对PDHK特异性的siRNA。
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公开(公告)号：US20210002669A1
公开(公告)日：2021-01-07
申请号：US16956942
申请日：2018-12-21
Applicant: GENENTECH, INC.
Inventor： Chi Kin Domingos Ng , Yongping Guo Crawford , Amy Shen , Meixia Zhou , Bradley R. Snedecor , Shahram Misaghi , Albert Eric Gao
Abstract: The presently disclosed subject matter relates to targeted integration (TI) host cells suitable for the expression of recombinant proteins, as well as methods of producing and using said TI host cells.
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公开(公告)号：US11046987B2
公开(公告)日：2021-06-29
申请号：US16888344
申请日：2020-05-29
Applicant: Genentech, Inc.
Inventor： Meixia Zhou , Bradley Richard Snedecor , Chi Kin Domingos Ng , Amy Shen
IPC: C12P21/00 , C12N15/113 , C12N1/38 , C12N15/11 , C12N15/85
Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.
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公开(公告)号：US10011856B2
公开(公告)日：2018-07-03
申请号：US15268424
申请日：2016-09-16
Applicant: Genentech, Inc.
Inventor： Meixia Zhou , Bradley Richard Snedecor , Chi Kin Domingos Ng , Amy Shen
CPC classification number: C12P21/00 , C12N1/38 , C12N15/111 , C12N15/113 , C12N15/1137 , C12N15/85 , C12N2310/14 , C12N2320/00 , C12Y101/01027 , C12Y102/04001
Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.
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