-
公开(公告)号：US12129480B2
公开(公告)日：2024-10-29
申请号：US16956942
申请日：2018-12-21
Applicant: GENENTECH, INC.
Inventor： Chi Kin Domingos Ng , Yongping Guo Crawford , Amy Shen , Meixia Zhou , Bradley R. Snedecor , Shahram Misaghi , Albert Eric Gao
CPC classification number: C12N15/907 , C07K16/00 , C12N15/90 , C07K2317/51 , C07K2317/515 , C12N2800/30 , C12N2800/90 , C12N2840/00
Abstract: The presently disclosed subject matter relates to targeted integration (TI) host cells suitable for the expression of recombinant proteins, as well as methods of producing and using said TI host cells.
-
公开(公告)号：US20240011066A1
公开(公告)日：2024-01-11
申请号：US18303451
申请日：2023-04-19
Applicant: Genentech, Inc. , Hoffmann-La Roche Inc.
Inventor： Shahram Misaghi , Amy Shen , Inn Huam Yuk , Gavin Christian Barnard , Simon Auslaender , Niels Bauer , Benedikt Oswald
CPC classification number: C12P21/02 , C12N15/907 , C12N15/11 , C12N9/93 , C12N9/22 , C12N9/1029 , C12Y203/01 , C12N9/86 , C12Y305/02006 , C12N2310/20 , C12N2800/80
Abstract: Herein are reported modified mammalian cells wherein the expression of the MYC gene and one or more of the BAK, BAX, SIRT-1 and ICAM-1 genes has been reduced or eliminated as well as methods for the recombinant production of a heterologous polypeptide using a modified mammalian cell according to the current invention. Further reported are use of the reduction of the expression of the genes for increasing volumetric productivity, increasing cell volume, increasing viability and increasing the possible cultivation time without cell split.
-
公开(公告)号：US11046987B2
公开(公告)日：2021-06-29
申请号：US16888344
申请日：2020-05-29
Applicant: Genentech, Inc.
Inventor： Meixia Zhou , Bradley Richard Snedecor , Chi Kin Domingos Ng , Amy Shen
IPC: C12P21/00 , C12N15/113 , C12N1/38 , C12N15/11 , C12N15/85
Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.
-
公开(公告)号：US10011856B2
公开(公告)日：2018-07-03
申请号：US15268424
申请日：2016-09-16
Applicant: Genentech, Inc.
Inventor： Meixia Zhou , Bradley Richard Snedecor , Chi Kin Domingos Ng , Amy Shen
CPC classification number: C12P21/00 , C12N1/38 , C12N15/111 , C12N15/113 , C12N15/1137 , C12N15/85 , C12N2310/14 , C12N2320/00 , C12Y101/01027 , C12Y102/04001
Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.
-
公开(公告)号：US20240409615A1
公开(公告)日：2024-12-12
申请号：US18752501
申请日：2024-06-24
Applicant: Genentech, Inc.
Inventor： Chi Kin Domingos Ng , Amy Shen , Gavin Christian Barnard
Abstract: The presently disclosed subject matter relates to multi-vector recombinase mediated cassette exchange approaches to achieve targeted integration of sequences of interest for the generation host cells expressing recombinant proteins, e.g., monoclonal antibodies, as well as compositions derived from the same, e.g., bispecific antibodies, and other complex format proteins, e.g., membrane protein complexes, and other difficult to express molecules.
-
Patent Agency Ranking
公开(公告)号：US20240254199A1
公开(公告)日：2024-08-01
申请号：US18489274
申请日：2023-10-18
Applicant: Genentech, Inc. , Hoffmann-La Roche Inc.
Inventor： Amy Shen , Inn Huam Yuk , Gavin Christian Barnard , Shahram Misaghi , Simon Auslaender , Niels Bauer , Benedikt Oswald
CPC classification number: C07K16/00 , C12N5/00 , C12N9/22 , C12N15/11 , C12N15/907 , C12P21/00 , C12N2310/20 , C12N2800/80
Abstract: The present disclosure relates to mammalian cells (e.g., Chinese Hamster Ovary (CHO) cells) that are modified to reduce or eliminate the expression of certain mammalian cell endogenous products (e.g., host cell proteins and virus-like particles), and methods of using such cells in the production of a recombinant product of interest, e.g., a recombinant protein, a recombinant viral particle, or a recombinant viral vector. These modifications were specifically chosen to generate engineered mammalian host cells with desired traits in several key areas, including improved cell culture performance (e.g., higher viability and product titers), improved product quality (e.g., more consistent and favorable glycosylation; more stable drug product), and decreased burden on purification for removing problematic or undesired endogenous host cell products (e.g., hydrolytic host cell proteins and virus-like particles) during biomanufacturing.
-
7.发明公开公开(公告)号：US20240240156A1
公开(公告)日：2024-07-18
申请号：US18510297
申请日：2023-11-15
Applicant: Genentech, Inc.
Inventor： Amy Shen , Gavin Christian Barnard , Shuet Ming Yip
CPC classification number: C12N5/0682 , C07K16/00 , C12N9/22 , C12N15/111 , C12N15/85 , C07K2317/622 , C12N2310/20 , C12N2840/002
Abstract: The presently disclosed subject matter relates to targeted integration (TI) host cells suitable for the expression of recombinant proteins where the TI host cells are also subjected to transposon-mediated genomic integration of one or more exogenous nucleic acids, as well as methods of producing and using said combined transposon-mediated genomic integration and TI host cells.
-
8.发明授权Decreasing lactate level and increasing polypeptide production by downregulating the expression of lactate dehydrogenase and pyruvate dehydrogenase kinase 有权通过下调乳酸脱氢酶和丙酮酸脱氢酶激酶的表达，降低乳酸水平和增加多肽产生公开(公告)号：US09487809B2
公开(公告)日：2016-11-08
申请号：US13688075
申请日：2012-11-28
Applicant: Genentech, Inc.
Inventor： Meixia Zhou , Bradley Richard Snedecor , Chi Kin Domingos Ng , Amy Shen
CPC classification number: C12P21/00 , C12N1/38 , C12N15/111 , C12N15/113 , C12N15/1137 , C12N15/85 , C12N2310/14 , C12N2320/00 , C12Y101/01027 , C12Y102/04001
Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.
Abstract translation: 本发明提供用于降低培养细胞中乳酸盐产生和增加多肽产生的方法和组合物。 一方面，本发明提供一种方法，其包括培养表达a）对乳酸脱氢酶（LDH）特异性的小干扰RNA（siRNA）的细胞和b）对丙酮酸脱氢酶激酶（PDHK）特异的siRNA。 在另一方面，本发明提供培养的细胞或载体，其包含对LDH特异性的siRNA和对PDHK特异性的siRNA。
-
9.发明申请DECREASING LACTATE LEVEL AND INCREASING POLYPEPTIDE PRODUCTION BY DOWNREGULATING THE EXPRESSION OF LACTATE DEHYDROGENASE AND PYRUVATE DEHYDROGENASE KINASE 审中-公开通过下调LACTATE DEHYDROGENASE和PYRUVATE DEHYDROGENASE KINASE的表达来降低LACTATE水平并增加多肽生产公开(公告)号：US20130084605A1
公开(公告)日：2013-04-04
申请号：US13688075
申请日：2012-11-28
Applicant: Genentech, Inc.
Inventor： Meixia Zhou , Bradley Richard Snedecor , Chi Kin Domingos Ng , Amy Shen
CPC classification number: C12P21/00 , C12N1/38 , C12N15/111 , C12N15/113 , C12N15/1137 , C12N15/85 , C12N2310/14 , C12N2320/00 , C12Y101/01027 , C12Y102/04001
Abstract: The present invention provides methods and compositions for reducing lactate production and increasing polypeptide production in cultured cells. In one aspect, the invention provides a method comprising culturing cells expressing a) a small interfering RNA (siRNA) specific for a lactate dehydrogenase (LDH) and b) an siRNA specific for a pyruvate dehydrogenase kinase (PDHK). In another aspect, the invention provides cultured cells or vectors comprising an siRNA specific for a LDH and an siRNA specific for a PDHK.
Abstract translation: 本发明提供用于降低培养细胞中乳酸盐产生和增加多肽产生的方法和组合物。 一方面，本发明提供一种方法，其包括培养表达a）对乳酸脱氢酶（LDH）特异性的小干扰RNA（siRNA）的细胞和b）对丙酮酸脱氢酶激酶（PDHK）特异的siRNA。 在另一方面，本发明提供培养的细胞或载体，其包含对LDH特异性的siRNA和对PDHK特异性的siRNA。
-
公开(公告)号：US20220162295A1
公开(公告)日：2022-05-26
申请号：US17383316
申请日：2021-07-22
Applicant: Hoffmann-La Roche Inc. , Genentech, Inc.
Inventor： Styliani Tournaviti , Shan-Hua Chung , Harald Duerr , Claudia Ferrara Koller , Amy Shen , Shahram Misaghi , Shuet Ming Yip
Abstract: Herein is reported a method for producing an antibody-multimer-fusion polypeptide comprising (a) an antibody heavy chain and an antibody light chain, and (b) a first fusion polypeptide comprising in N- to C-terminal direction a first part of a non-antibody multimeric polypeptide, an antibody heavy chain CH1 domain or an antibody light chain constant domain, an antibody hinge region, an antibody heavy chain CH2 domain and an antibody heavy chain CH3 domain, and a second fusion polypeptide comprising in N- to C-terminal direction the second part of the non-antibody multimeric polypeptide and an antibody light chain constant domain if the first polypeptide comprises an antibody heavy chain CH1 domain or an antibody heavy chain CH1 domain if the first polypeptide comprises an antibody light chain constant domain, wherein (i) the antibody heavy chain of (a) and the first fusion polypeptide of (b), (ii) the antibody heavy chain of (a) and the antibody light chain of (a), and (iii) the first fusion polypeptide of (b) and the second fusion polypeptide of (b) are each independently of each other covalently linked to each other by at least one disulfide bond, characterized in that the antibody-multimer-fusion is expressed by a recombinant mammalian cell obtained by transfecting a mammalian cell with the expression cassettes for the antibody heavy chain, the antibody light chain, the first fusion polypeptide and the second fusion polypeptide at a stoichiometric ratio of 1:1:2:1.
-
-
-
-
-
-
-
-
-
Search Results
13
records
(took 0.02 seconds)
