公开(公告)号：US09409989B2

公开(公告)日：2016-08-09

申请号：US14142663

申请日：2013-12-27

Applicant: GENENTECH, INC.

Inventor： W. Robert Arathoon ,  Paul J. Carter ,  Anne M. Merchant ,  Leonard G. Presta

IPC: C07H21/02 ,  C07H21/04 ,  C12N5/10 ,  C12N15/09 ,  C12N15/13 ,  C12N15/63 ,  C07K16/46 ,  C07K16/28 ,  C07K1/22 ,  A61K38/00

CPC classification number: C07K16/2863 ,  A61K38/00 ,  C07K1/22 ,  C07K16/2809 ,  C07K16/2812 ,  C07K16/46 ,  C07K16/468 ,  C07K2317/732 ,  Y10S435/972

Abstract: The invention relates to a method of preparing heteromultimeric polypeptides such as bispecific antibodies, bispecific immunoadhesins and antibody-immunoadhesin chimeras. The invention also relates to the heteromultimers prepared using the method. Generally, the method provides a multispecific antibody having a common light chain associated with each heteromeric polypeptide having an antibody binding domain. Additionally the method further involves introducing into the multispecific antibody a specific and complementary interaction at the interface of a first polypeptide and the interface of a second polypeptide, so as to promote heteromultimer formation and hinder homomultimer formation; and/or a free thiol-containing residue at the interface of a first polypeptide and a corresponding free thiol-containing residue in the interface of a second polypeptide, such that a non-naturally occurring disulfide bond is formed between the first and second polypeptide. The method allows for the enhanced formation of the desired heteromultimer relative to undesired heteromultimers and homomultimers.

Abstract translation: 本发明涉及制备异源多聚体多肽如双特异性抗体，双特异性免疫粘附素和抗体免疫粘附素嵌合体的方法。 本发明还涉及使用该方法制备的异源多聚体。 通常，该方法提供具有与具有抗体结合结构域的每个异聚多肽相关联的共同轻链的多特异性抗体。 另外，该方法还包括将多特异性抗体引入第一多肽的界面处的第二多肽的界面上的特异性和互补相互作用，以促进异源多聚体形成并阻碍同源多聚体形成; 和/或在第二多肽的界面处的第一多肽和相应的游离含硫醇残基的界面处的游离含硫醇残基，使得在第一和第二多肽之间形成非天然存在的二硫键。 该方法允许相对于不想要的异多元反应物和同源多聚体增强所需异源多聚体的形成。

公开(公告)号：US20150023951A1

公开(公告)日：2015-01-22

申请号：US14171616

申请日：2014-02-03

Applicant: Genentech, Inc.

Inventor： Manuel Baca ,  James A. Wells ,  Leonard G. Presta ,  Henry B. Lowman ,  Yvonne Man-yee Chen

IPC: C07K16/22

CPC classification number: C07K16/22 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/76 ,  C07K2317/92

Abstract: Humanized and variant anti-VEGF antibodies and various uses therefor are disclosed. The anti-VEGF antibodies have strong binding affinities for VEGF; inhibit VEGF-induced proliferation of endothelial cells in vitro; and inhibit tumor growth in vivo.

公开(公告)号：US09493579B2

公开(公告)日：2016-11-15

申请号：US14229683

申请日：2014-03-28

Applicant: GENENTECH, INC.

Inventor： Kathy L. Miller ,  Leonard G. Presta

IPC: C07K16/00 ,  C07K16/46 ,  C07K16/28 ,  C07K16/32 ,  A61K39/395 ,  A61K45/06 ,  A61K47/48 ,  A61K39/00

CPC classification number: C07K16/468 ,  A61K39/3955 ,  A61K45/06 ,  A61K47/6879 ,  A61K2039/505 ,  C07K16/00 ,  C07K16/2878 ,  C07K16/2896 ,  C07K16/32 ,  C07K2317/55 ,  C07K2317/64 ,  C07K2317/73 ,  C07K2317/77 ,  C07K2319/00

Abstract: The present application describes engineered antibodies, with three or more functional antigen binding sites, and uses, such as therapeutic applications, for such engineered antibodies.

公开(公告)号：US20130309236A1

公开(公告)日：2013-11-21

申请号：US13956266

申请日：2013-07-31

Applicant: Genentech, Inc.

Inventor： Anan CHUNTHARAPAI ,  Jin K. Kim ,  Leonard G. Presta ,  Timothy Stewart

IPC: C07K16/24

CPC classification number: C07K16/249 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/76 ,  C07K2317/92 ,  C07K2319/00 ,  G01N33/564 ,  G01N33/6866 ,  G01N2333/56 ,  G01N2800/042 ,  G01N2800/24

Abstract: The present invention relates generally to the generation and characterization of neutralizing anti-IFN-α monoclonal antibodies with broad reactivity against various IFN-α subtypes. The invention further relates to the use of such anti-IFN-α antibodies in the diagnosis and treatment of disorders associated with increased expression of TUN-α, in particular, autoimmune disorders such as insulin-dependent diabetes mellitus (IDDM) and systemic lupus erythematosus (SLE).

Abstract translation: 本发明一般涉及对各种IFN-α亚型具有广泛反应性的中和抗IFN-α单克隆抗体的产生和表征。 本发明还涉及这样的抗-IFN-α抗体在诊断和治疗与TUN-α表达增加相关的疾病中的用途，特别是自身免疫性疾病如胰岛素依赖性糖尿病（IDDM）和系统性红斑狼疮 （SLE）。

公开(公告)号：US20130058927A1

公开(公告)日：2013-03-07

申请号：US13665810

申请日：2012-10-31

Applicant: GENENTECH, INC.

Inventor： Manuel Baca ,  James A. Wells ,  Leonard G. Presta ,  Henry B. Lowman ,  Yvonne Man-Yee Chen

IPC: A61K39/395 ,  C07K16/22 ,  C12N15/62 ,  A61P35/00 ,  A61P27/02 ,  C12N5/10 ,  C12N15/63 ,  C12P21/02 ,  C12N1/21 ,  C12N1/19 ,  C12N1/15 ,  C07K16/46 ,  A61P9/00

CPC classification number: C07K16/22 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92

Abstract: Humanized and variant anti-VEGF antibodies and various uses therefor are disclosed. The anti-VEGF antibodies have strong binding affinities for VEGF; inhibit VEGF-induced proliferation of endothelial cells in vitro; and inhibit tumor growth in vivo.

公开(公告)号：US20140154242A1

公开(公告)日：2014-06-05

申请号：US14029717

申请日：2013-09-17

Applicant: Genentech, Inc.

Inventor： Camellia W. ADAMS ,  Andrew C. Chan ,  Craig W. Crowley ,  Henry B. Lowman ,  Gerald R. Nakamura ,  Leonard G. Presta

IPC: C07K16/28

CPC classification number: C07K16/2887 ,  A61K2039/505 ,  C07K2317/24 ,  C07K2317/41 ,  C07K2317/52 ,  C07K2317/522 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/72 ,  C07K2317/73 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/75 ,  C07K2317/92

Abstract: The invention provides humanized and chimeric anti-CD20 antibodies for treatment of CD20 positive malignancies and autoimmune diseases.

Abstract translation: 本发明提供用于治疗CD20阳性恶性肿瘤和自身免疫疾病的人源化和嵌合抗CD20抗体。

公开(公告)号：US20170369564A1

公开(公告)日：2017-12-28

申请号：US14997205

申请日：2016-01-15

Applicant: Genentech, Inc.

Inventor： Manuel Baca ,  James A. Wells ,  Leonard G. Presta ,  Henry B. Lowman ,  Yvonne Man-yee Chen

IPC: C07K16/22

CPC classification number: C07K16/22 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/76 ,  C07K2317/92

Abstract: Humanized and variant anti-VEGF antibodies and various uses therefor are disclosed. The anti-VEGF antibodies have strong binding affinities for VEGF; inhibit VEGF-induced proliferation of endothelial cells in vitro; and inhibit tumor growth in vivo.

公开(公告)号：US20160257738A1

公开(公告)日：2016-09-08

申请号：US14876021

申请日：2015-10-06

Applicant: GENENTECH, INC.

Inventor： Manuel Baca ,  James A. Wells ,  Leonard G. Presta ,  Henry B. Lowman ,  Yvonne Man-Yee Chen

IPC: C07K16/22

CPC classification number: C07K16/22 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/73 ,  C07K2317/76 ,  C07K2317/92

Abstract: Humanized and variant anti-VEGF antibodies and various uses therefor are disclosed. The anti-VEGF antibodies have strong binding affinities for VEGF; inhibit VEGF-induced proliferation of endothelial cells in vitro; and inhibit tumor growth in vivo.