公开(公告)号：US10030241B2

公开(公告)日：2018-07-24

申请号：US15150806

申请日：2016-05-10

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Patrick McCoy Spooner ,  Peter James Tatnell ,  Jeffrey Kenneth Horton ,  John Richard Nelson ,  Michael John Gerdes ,  Suzana Kiel ,  Ralf Lenigk ,  Alexander Schenk ,  Wei Sun

IPC: C12N15/10 ,  G01N33/53 ,  C12Q1/6804 ,  G01N27/447 ,  G01N33/68 ,  C12Q1/6888

Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method includes, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.

公开(公告)号：US20160289666A1

公开(公告)日：2016-10-06

申请号：US15150806

申请日：2016-05-10

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Patrick McCoy Spooner ,  Peter James Tatnell ,  Jeffrey Kenneth Horton ,  John Richard Nelson ,  Michael John Gerdes ,  Suzana Kiel ,  Ralf Lenigk ,  Alexander Schenk ,  Wei Sun

IPC: C12N15/10 ,  C12Q1/68 ,  G01N27/447 ,  G01N33/53

CPC classification number: C12N15/1013 ,  C12N15/101 ,  C12Q1/6804 ,  C12Q1/6888 ,  C12Q2600/124 ,  G01N27/44743 ,  G01N27/44747 ,  G01N33/5308 ,  G01N33/689 ,  C12Q2522/101

Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method comprises, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.

Abstract translation: 本发明一般涉及从生物样品中或在生物样品中捕获精子核酸的方法和试剂盒。 在一个实施方案中，该方法包括使样品与裂解溶液接触，其具有鱼精蛋白-DNA复合物，以裂解细胞并施用鱼精蛋白特异性抗体。 这导致鱼精蛋白特异性抗体结合鱼精蛋白-DNA以形成可被捕获，纯化或检测的复合物。 还提供了用于实施所公开的方法的套件。

公开(公告)号：US11698427B2

公开(公告)日：2023-07-11

申请号：US17499295

申请日：2021-10-12

Applicant: General Electric Company

Inventor： Anand Kumar Venkatachari ,  Ling Sun ,  Wei Sun

IPC: G01R33/44 ,  G01R33/54 ,  G01R33/483 ,  G01R33/36

CPC classification number: G01R33/443 ,  G01R33/3614 ,  G01R33/4835 ,  G01R33/543

Abstract: Various methods and systems are provided for correcting transmit attenuation of an amplifier of a transmit radio frequency (RF) coil for use in a magnetic resonance imaging (MRI) system. In one example, a method includes setting a reference value of transmit attenuation for an amplifier of a transmit radio frequency (RF) coil, acquiring a three-dimensional B1 field map with the transmit attenuation set at the reference value, determining a plurality of mean flip angles for a plurality of slice locations in a pre-scan imaging volume from the B1 field map, determining a transmit attenuation correction value for each of the slice locations based on a prescribed flip angle and the mean flip angle determined for the respective slice location, correcting the reference value of transmit attenuation with the transmit attenuation correction value at each of the slice locations to obtain a final value of transmit attenuation for each of the slice locations, and performing an MRI scan with the transmit attenuation set at the value.

公开(公告)号：US20200049786A1

公开(公告)日：2020-02-13

申请号：US16101199

申请日：2018-08-10

Applicant: General Electric Company

Inventor： Anand Kumar Venkatachari ,  Ling Sun ,  Wei Sun

IPC: G01R33/58 ,  G01R33/24 ,  G01R33/565 ,  G01R33/46

Abstract: Various methods and systems are provided for correcting transmit attenuation of an amplifier of a transmit radio frequency (RF) coil for use in a magnetic resonance imaging (MRI) system. In one example, a method includes setting a reference value of transmit attenuation for an amplifier of a transmit radio frequency (RF) coil, acquiring a two-dimensional B1 field map with the transmit attenuation set at the reference value, determining a mean flip angle from the B1 field map, determining a transmit attenuation correction value based on a prescribed flip angle and the mean flip angle, correcting the reference value of transmit attenuation with the transmit attenuation correction value to obtain a final value of transmit attenuation, and performing an MRI scan with the transmit attenuation set at the value.

公开(公告)号：US20220026511A1

公开(公告)日：2022-01-27

申请号：US17499295

申请日：2021-10-12

Applicant: General Electric Company

Inventor： Anand Kumar Venkatachari ,  Ling Sun ,  Wei Sun

IPC: G01R33/44 ,  G01R33/36 ,  G01R33/483 ,  G01R33/54

Abstract: Various methods and systems are provided for correcting transmit attenuation of an amplifier of a transmit radio frequency (RF) coil for use in a magnetic resonance imaging (MRI) system. In one example, a method includes setting a reference value of transmit attenuation for an amplifier of a transmit radio frequency (RF) coil, acquiring a three-dimensional B1 field map with the transmit attenuation set at the reference value, determining a plurality of mean flip angles for a plurality of slice locations in a pre-scan imaging volume from the B1 field map, determining a transmit attenuation correction value for each of the slice locations based on a prescribed flip angle and the mean flip angle determined for the respective slice location, correcting the reference value of transmit attenuation with the transmit attenuation correction value at each of the slice locations to obtain a final value of transmit attenuation for each of the slice locations, and performing an MRI scan with the transmit attenuation set at the value.

公开(公告)号：US11175367B2

公开(公告)日：2021-11-16

申请号：US16101199

申请日：2018-08-10

Applicant: General Electric Company

Inventor： Anand Kumar Venkatachari ,  Ling Sun ,  Wei Sun

IPC: G01R33/58 ,  G01R33/46 ,  G01R33/565 ,  G01R33/24

Abstract: Various methods and systems are provided for correcting transmit attenuation of an amplifier of a transmit radio frequency (RF) coil for use in a magnetic resonance imaging (MRI) system. In one example, a method includes setting a reference value of transmit attenuation for an amplifier of a transmit radio frequency (RF) coil, acquiring a two-dimensional B1 field map with the transmit attenuation set at the reference value, determining a mean flip angle from the B1 field map, determining a transmit attenuation correction value based on a prescribed flip angle and the mean flip angle, correcting the reference value of transmit attenuation with the transmit attenuation correction value to obtain a final value of transmit attenuation, and performing an MRI scan with the transmit attenuation set at the value.

公开(公告)号：US09778333B2

公开(公告)日：2017-10-03

申请号：US13900678

申请日：2013-05-23

Applicant: GENERAL ELECTRIC COMPANY

Inventor： Wei Sun ,  Gaohong G. Wu ,  Jason Andrew Polzin ,  Ajit Shankaranarayanan

IPC: G01R33/385 ,  G01R33/563 ,  G01R33/48

CPC classification number: G01R33/3854 ,  G01R33/4826 ,  G01R33/5635 ,  G01R33/56366

Abstract: Systems and methods for reducing acoustic noise in a Magnetic Resonance Imaging (MRI) are provided. One method includes applying a labeling phase of an arterial spin labeling (ASL) pulse sequence to a region of interest, applying a three-dimensional (3D) radial pulse sequence to the region of interest to generate a tag image, applying a control phase of the ASL pulse sequence to the region of interest, and applying the 3D radial pulse sequence to the region of interest to generate a control image.