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公开(公告)号：US20210002688A1
公开(公告)日：2021-01-07
申请号：US16459336
申请日：2019-07-01
Applicant: General Electric Company
Inventor： Alex David Corwin , Erik Leeming Kvam , Christine Lynne Surrette , Brian Joseph Scherer , Pei-Hsin Kuo , Ralf Lenigk
Abstract: The present disclosure relates to approaches for assessing a sample or the presence of microorganisms. The sample, in certain implementations may be assessed for one or both of absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). sample partition device may be employed that partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.
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公开(公告)号：US10859563B2
公开(公告)日：2020-12-08
申请号：US14956067
申请日：2015-12-01
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Jessica Godin Karp , Xiaohui Chen , Kashan Ali Shaikh , Mengli Wang , Ralf Lenigk , Christine Lynne Surrette
Abstract: A device and/or methodology are described that include a mechanism for separating erythrocytes from other constituents of blood and for purifying leukocytes from blood. The separation and purification aspects may be provided in separate components or within the same component. The separation aspect assists in separating erythrocytes (red blood cells) from other cells in blood, such as by aggregation of the red blood cells. A suitable aggregation device or device component uses chambers with at least one small dimension (e.g., a microfluidic chip) to control the interaction of the blood with a solution containing a high molecular weight polymer (e.g., dextran) to achieve separation.
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公开(公告)号：US10598577B2
公开(公告)日：2020-03-24
申请号：US15639511
申请日：2017-06-30
Applicant: General Electric Company , University of Akron
Inventor： Arunkumar Natarajan , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Wei Gao , Kwok Pong Chan , Lakshmi Sireesha Kaanumalle , Abraham Joy , Nicholas Nun
Abstract: A method includes providing a biological sample, providing a sample collection device, wherein the sample collection device includes a sample binding surface including a photodegradable polymer configured to bind the biological sample, contacting the biological sample with the sample binding surface of the sample collection device, and irradiating the sample binding surface and the bound biological sample using light emitted from a light source to initiate degradation of the photodegradable polymer of the sample binding surface to cause release of the biological sample.
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公开(公告)号：US20180361317A1
公开(公告)日：2018-12-20
申请号：US16012147
申请日：2018-06-19
Applicant: General Electric Company
Inventor： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk , Nicole Lea Wood , Li Zhu , Craig Patrick Galligan
Abstract: A method of eluting biomolecules, such as nucleic acids from a biological sample by electroelution is provided. An example of a method includes various steps, such as loading the biological sample to a device comprising a housing, at least two conductive redox polymer electrodes operationally coupled to the housing and a biomolecule impermeable layer disposed on at least one of the electrodes. The loading of sample is followed by initiating an electrical connection to generate an electric field strength sufficient to elute biomolecules from the biological sample; and eluting the biomolecules from the biological sample.
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公开(公告)号：US10033910B2
公开(公告)日：2018-07-24
申请号：US15130401
申请日：2016-04-15
Applicant: General Electric Company
Inventor： Ralf Lenigk , Mark Marshall Meyers , Victor Petrovich Ostroverkhov , Timothy Toepfer , Keith Michael Looney
Abstract: The present approach relates to the synchronization of frame acquisition by a camera with an external event or trigger despite the camera lacking external control or synchronization capabilities. For example, inexpensive and/or consumer grade camera typically lack a control interface to explicitly synchronize with an external trigger event or external device. The present approach allows synchronization of such a camera lacking external synchronization capabilities with an external event or device.
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Patent Agency Ranking
公开(公告)号：US20160023209A1
公开(公告)日：2016-01-28
申请号：US14340693
申请日：2014-07-25
Applicant: General Electric Company
Inventor： Ralf Lenigk , Erin Jean Finehout , Xuefeng Wang
IPC: B01L3/00
CPC classification number: B01L3/502715 , B01L3/0258 , B01L3/0262 , B01L3/5023 , B01L3/561 , B01L2200/027 , B01L2200/10 , B01L2300/069 , B01L2300/0838 , B01L2300/0887 , B01L2300/12 , B01L2300/161 , B01L2300/165 , B01L2400/0406
Abstract: An integrated device for a sample collection and transfer is provided. The integrated device comprises a capillary channel disposed between a first layer and a second layer, wherein the first layer comprises a hydrophilic layer comprising a fluid inlet for receiving a sample fluid to the capillary channel, wherein the capillary channel comprises an inner surface and an outer surface; and an outlet for driving out the sample fluid. The device further comprises a third layer comprising an adhesive material such as a patterned adhesive material, and a flow path, wherein the third layer is disposed on the outer surface of the capillary, at a determining position relative to the outlet, such that the capillary is in contact with the third layer and the outlet is in contact with the flow path of the third layer for allowing the sample fluid out from the integrated device.
Abstract translation: 提供了一种用于样品收集和转移的集成设备。 集成装置包括设置在第一层和第二层之间的毛细通道，其中第一层包括亲水层，该亲水层包括用于将样品流体接收到毛细通道的流体入口，其中毛细通道包括内表面和外表面 表面; 以及用于驱出样品流体的出口。 该装置还包括第三层，其包括诸如图案化粘合剂材料的粘合剂材料和流动路径，其中第三层设置在毛细管的外表面上，在相对于出口的确定位置处，使得毛细管 与第三层接触，并且出口与第三层的流动路径接触，以允许样品流体从集成装置流出。
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公开(公告)号：US20140154736A1
公开(公告)日：2014-06-05
申请号：US13690801
申请日：2012-11-30
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Christopher Michael Puleo , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk
CPC classification number: G01N1/30 , A01N1/0242 , A01N1/0284 , B01L3/5023 , B01L3/5088 , B01L7/00 , B01L2200/0678 , B01L2300/0825 , B01L2300/126 , B01L2300/1877 , G01N2001/4027 , Y10T436/2525
Abstract: A method of drying a biological sample disposed on a substrate is provided. The method comprises providing the substrate comprising a sample loading area and a heat source; activating the heat source for generating heat; heating the substrate at least above 65° C.; and drying the biological sample. A device for storing sample is also provided, wherein the device comprises a substrate for biological sample-storage; and a heating component that generates heat to maintain a temperature of at least above 65° C. The heating component may contain one or more reagents, wherein the reagents generate heat to maintain a temperature of at least above 65° C.
Abstract translation: 提供了一种干燥设置在基板上的生物样品的方法。 该方法包括提供包括样品加载区域和热源的基底; 激活热源产生热量; 将衬底加热至少高于65℃; 并干燥生物样品。 还提供了用于存储样品的装置，其中所述装置包括用于生物样品储存的基底; 以及产生热量以保持至少高于65℃的温度的加热组分。加热组分可以含有一种或多种试剂，其中试剂产生热量以保持至少高于65℃的温度。
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公开(公告)号：US11590502B2
公开(公告)日：2023-02-28
申请号：US16459363
申请日：2019-07-01
Applicant: General Electric Company
Inventor： Alex David Corwin , Erik Leeming Kvam , Christine Lynne Surrette , Brian Joseph Scherer , Pei-Hsin Kuo , Ralf Lenigk , Tyler John Hammond
Abstract: The present disclosure relates to a consumable sample partition device and it assembly and use. The sample partition device can be used to test a sample for absence of microorganisms (sterility) and/or for concentration of said organisms (bio-burden). The sample partition device partitions the sample input volume into multiple discrete measurement zones with little or no loss of sample (e.g., zero-loss) and with little operator involvement, thereby reducing operator- and environment-based false positives.
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公开(公告)号：US10030241B2
公开(公告)日：2018-07-24
申请号：US15150806
申请日：2016-05-10
Applicant: GENERAL ELECTRIC COMPANY
Inventor： Patrick McCoy Spooner , Peter James Tatnell , Jeffrey Kenneth Horton , John Richard Nelson , Michael John Gerdes , Suzana Kiel , Ralf Lenigk , Alexander Schenk , Wei Sun
IPC: C12N15/10 , G01N33/53 , C12Q1/6804 , G01N27/447 , G01N33/68 , C12Q1/6888
Abstract: The invention generally relates to methods and kits for capturing sperm nucleic acids from or in a biological sample. In one embodiment the method the method includes, contacting the sample with a lysis solution, having a protamine-DNA complex, to lyse the cell and applying a protamine-specific antibody. This results in the protamine-specific antibody binding to the protamine-DNA to form a complex which may be captured, purified, or detected. Also provided are kits for carrying out the disclosed methods.
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公开(公告)号：US09957548B2
公开(公告)日：2018-05-01
申请号：US14672358
申请日：2015-03-30
Applicant: General Electric Company
Inventor： Michael John Gerdes , John Richard Nelson , Patrick McCoy Spooner , Ralf Lenigk
CPC classification number: C12Q1/6804 , C12N15/1003 , C12Q2522/101 , G01N33/689
Abstract: A method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample is disclosed. The method includes a step of contacting a lysis solution to the sample that includes at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate includes a protamine-DNA complex. The method further includes applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The method further includes capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex.
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