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公开(公告)号：US20160194632A1
公开(公告)日：2016-07-07
申请号：US14911618
申请日：2014-09-23
IPC分类号： C12N15/113 , A01N25/00 , A01N25/34 , A01N57/16
CPC分类号： C12N15/113 , A01N25/006 , A01N25/34 , A01N57/16 , C12N15/111 , C12N15/88 , C12N2310/14 , C12N2320/32 , C12N2330/50 , C12N2795/18123 , C12N2795/18142
摘要： A broad and extensive new category of targets for ribonucleic acids (RNAs) with interference activity (iRNAs), exclusive of the traditional messenger RNA (mRNA) targets have been discovered. iRNAs can be used to manipulate biological processes that do not explicitly involve mRNA and can be directed at non-coding RNAs, such as ribosomal RNAs (rRNAs) and transfer RNAs (tRNAs). iRNA sequences targeted at ribosomal rRNAs and tRNAs have been designed and tested. iRNA that targets a non-coding RNA is called non-coding interfering RNA (nciRNA). nciRNAs cause degradation of non-coding RNAs in vivo, and are highly active in biological assays. nciRNAs can be used as programmed toxins for specific targeting of eukaryotic pathogens and for protection of plants and structures from insects and weeds.
摘要翻译： 已经发现了广泛和广泛的新类别的具有干扰活性（iRNA）的核糖核酸（RNA）的目标，不包括传统的信使RNA（mRNA）靶。 iRNA可用于操作不明确涉及mRNA的生物过程，并且可以针对非编码RNA，例如核糖体RNA（rRNA）和转移RNA（tRNA）。 已经设计和测试了靶向核糖体rRNA和tRNA的iRNA序列。 靶向非编码RNA的iRNA称为非编码干扰RNA（nciRNA）。 nciRNA在体内导致非编码RNA的降解，并且在生物测定中具有高度的活性。 nciRNA可用作特异性靶向真核病原体的程序毒素，并用于保护植物和结构免受昆虫和杂草的侵害。
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公开(公告)号：US09932563B2
公开(公告)日：2018-04-03
申请号：US14911645
申请日：2014-09-11
CPC分类号： C12N7/00 , A01N57/16 , C12N15/1137 , C12N15/86 , C12N15/88 , C12N2310/14 , C12N2795/18123 , C12N2795/18142 , C12N2795/18151 , C12N2795/18152
摘要： A combined packing and assembly method that efficiently packs ribonucleic acid (RNA) into virus like particles (VLPs) has been developed. The VLPs can spontaneously assemble and load RNA in vivo, efficiently packaging specifically designed RNAs at high densities and with high purity. In some embodiments the RNA is capable of interference activity, or is a precursor of a RNA capable of causing interference activity. Compositions and methods for the efficient expression, production and purification of VLP-RNAs are provided. VLP-RNAs can be used for the storage of RNA for long periods, and provide the ability to deliver RNA in stable form that is readily taken up by cells.
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公开(公告)号：US10329560B2
公开(公告)日：2019-06-25
申请号：US14911618
申请日：2014-09-23
摘要： A broad and extensive new category of targets for ribonucleic acids (RNAs) with interference activity (iRNAs), exclusive of the traditional messenger RNA (mRNA) targets have been discovered. iRNAs can be used to manipulate biological processes that do not explicitly involve mRNA and can be directed at non-coding RNAs, such as ribosomal RNAs (rRNAs) and transfer RNAs (tRNAs). iRNA sequences targeted at ribosomal rRNAs and tRNAs have been designed and tested. iRNA that targets a non-coding RNA is called non-coding interfering RNA (nciRNA). nciRNAs cause degradation of non-coding RNAs in vivo, and are highly active in biological assays. nciRNAs can be used as programmed toxins for specific targeting of eukaryotic pathogens and for protection of plants and structures from insects and weeds.
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公开(公告)号：US20160194613A1
公开(公告)日：2016-07-07
申请号：US14911645
申请日：2014-09-11
IPC分类号： C12N7/00 , A01N57/16 , C12N15/113
CPC分类号： C12N7/00 , A01N57/16 , C12N15/1137 , C12N15/86 , C12N15/88 , C12N2310/14 , C12N2795/18123 , C12N2795/18142 , C12N2795/18151 , C12N2795/18152
摘要： A combined packing and assembly method that efficiently packs ribonucleic acid (RNA) into virus like particles (VLPs) has been developed. The VLPs can spontaneously assemble and load RNA in vivo, efficiently packaging specifically designed RNAs at high densities and with high purity. In some embodiments the RNA is capable of interference activity, or is a precursor of a RNA capable of causing interference activity. Compositions and methods for the efficient expression, production and purification of VLP-RNAs are provided. VLP-RNAs can be used for the storage of RNA for long periods, and provide the ability to deliver RNA in stable form that is readily taken up by cells.
摘要翻译： 已经开发了将核糖核酸（RNA）有效地包装到病毒样颗粒（VLPs）中的组合包装和装配方法。 VLP可以在体内自发组装和加载RNA，以高密度和高纯度有效地包装专门设计的RNA。 在一些实施方案中，RNA能够干扰活性，或者是能够引起干扰活性的RNA的前体。 提供了VLP-RNA有效表达，生产和纯化的组合物和方法。 VLP-RNA可用于长时间保存RNA，并提供以细胞容易吸收的稳定形式提供RNA的能力。
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