公开(公告)号：US10336794B2

公开(公告)日：2019-07-02

申请号：US15698434

申请日：2017-09-07

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Jeannette Adu-Bobie ,  Mariagrazia Pizza ,  Nathalie Norais ,  Germano Ferrari ,  Guido Grandi

IPC: A61K39/02 ,  C07K14/22 ,  C07K14/245 ,  A61K39/095 ,  C12N9/10 ,  A61K39/108 ,  A61K39/00

Abstract: Knockout of the meningococcal mltA homolog gives bacteria that spontaneously release vesicles that are rich in immunogenic outer membrane proteins and that can elicit cross-protective antibody responses with higher bactericidal titres than OMVs prepared by normal production processes. Thus the invention provides a bacterium having a knockout mutation of its mltA gene. The invention also provides a bacterium, wherein the bacterium: (i) has a cell wall that includes peptidoglycan; and (ii) does not express a protein having the lytic transglycosylase activity MltA protein. The invention also provides compositions comprising vesicles that, during culture of bacteria of the invention, are released into the culture medium.

公开(公告)号：US09771399B2

公开(公告)日：2017-09-26

申请号：US14927052

申请日：2015-10-29

Applicant: GlaxoSmithKline Biologicals SA

Inventor： Jeannette Adu-Bobie ,  Mariagrazia Pizza ,  Nathalie Norais ,  Germano Ferrari ,  Guido Grandi

IPC: C07K14/22 ,  A61K39/095 ,  C07K14/245 ,  C12N9/10 ,  A61K39/108 ,  A61K39/00

CPC classification number: C07K14/245 ,  A61K39/00 ,  A61K39/0258 ,  A61K39/095 ,  C07K14/22 ,  C12N9/1051 ,  Y02A50/474

Abstract: Knockout of the meningococcal mltA homolog gives bacteria that spontaneously release vesicles that are rich in immunogenic outer membrane proteins and that can elicit cross-protective antibody responses with higher bactericidal titers than OMVs prepared by normal production processes. Thus the invention provides a bacterium having a knockout mutation of its mltA gene. The invention also provides a bacterium, wherein the bacterium: (i) has a cell wall that includes peptidoglycan; and (ii) does not express a protein having the lytic transglycosylase activity MltA protein. The invention also provides compositions comprising vesicles that, during culture of bacteria of the invention, are released into the culture medium.