公开(公告)号：US20160376301A1

公开(公告)日：2016-12-29

申请号：US15258881

申请日：2016-09-07

Applicant: GlaxoSmithKline Biologicals SA

Inventor： Paolo COSTANTINO ,  Maria Rosaria ROMANO ,  Francesco BERTI

IPC: C07H1/08 ,  C12P19/04 ,  A61K39/085

CPC classification number: C07H1/08 ,  A61K39/085 ,  A61K2039/55505 ,  A61K2039/6037 ,  C12P19/04

Abstract: The invention provides a method for releasing capsular polysaccharide from S. aureus type 5 or type 8 cells, comprising the step of treating the cells with acid. The invention further provides a process for purifying capsular polysaccharide from S. aureus type 5 or type 8 cells comprising this method. Other processing steps may be included in the process, such as enzymatic treatment, e.g. to remove nucleic acid, protein and/or peptidoglycan contaminants; diafiltration, e.g. to remove low molecular weight contaminants; anion exchange chromatography, e.g. to remove residual protein; and concentration.

Abstract translation: 本发明提供了一种从金黄色葡萄球菌5型或8型细胞中释放荚膜多糖的方法，包括用酸处理细胞的步骤。 本发明还提供了一种从包括该方法的金黄色葡萄球菌5型或8型细胞中纯化荚膜多糖的方法。 其他处理步骤可以包括在该方法中，例如酶处理，例如， 去除核酸，蛋白质和/或肽聚糖污染物; 渗滤，例如 去除低分子量污染物; 阴离子交换层析，例如 去除残留蛋白质; 和集中。

公开(公告)号：US20150315225A1

公开(公告)日：2015-11-05

申请号：US14714097

申请日：2015-05-15

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Paolo COSTANTINO ,  Maria Rosaria ROMANO ,  Francesco BERTI

IPC: C07H1/08 ,  C12P19/04 ,  A61K39/085

CPC classification number: C07H1/08 ,  A61K39/085 ,  A61K2039/55505 ,  A61K2039/6037 ,  C12P19/04

Abstract: The invention provides a method for releasing capsular polysaccharide from S. aureus type 5 or type 8 cells, comprising the step of treating the cells with acid. The invention further provides a process for purifying capsular polysaccharide from S. aureus type 5 or type 8 cells comprising this method. Other processing steps may be included in the process, such as enzymatic treatment, e.g. to remove nucleic acid, protein and/or peptidoglycan contaminants; diafiltration, e.g. to remove low molecular weight contaminants; anion exchange chromatography, e.g. to remove residual protein; and concentration.

Abstract translation: 本发明提供了一种从金黄色葡萄球菌5型或8型细胞中释放荚膜多糖的方法，包括用酸处理细胞的步骤。 本发明还提供了一种从包括该方法的金黄色葡萄球菌5型或8型细胞中纯化荚膜多糖的方法。 其他处理步骤可以包括在该方法中，例如酶处理，例如， 去除核酸，蛋白质和/或肽聚糖污染物; 渗滤，例如 去除低分子量污染物; 阴离子交换层析，例如 去除残留蛋白质; 和集中。

公开(公告)号：US20230346905A1

公开(公告)日：2023-11-02

申请号：US18042355

申请日：2021-08-23

Applicant: GlaxosmithKline Biologicals SA

Inventor： Roberto ADAMO ,  Maria Rosaria ROMANO ,  Marta TONTINI

IPC: A61K39/095 ,  A61P31/04

CPC classification number: A61K39/095 ,  A61P31/04 ,  A61K2039/70

Abstract: The inventors have identified a combined vaccine for immunisation against bacterial meningitis caused by multiple pathogens.

公开(公告)号：US20230210975A1

公开(公告)日：2023-07-06

申请号：US18008777

申请日：2021-06-11

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Roberto ADAMO ,  Filippo CARBONI ,  Roberta COZZI ,  Immaculada MARGARIT Y ROS ,  Maria Rosaria ROMANO

IPC: A61K39/09 ,  A61P31/04

CPC classification number: A61K39/092 ,  A61P31/04 ,  A61K2039/6031

Abstract: Methods of inducing an immunogenic response against a bacterial polysaccharide or oligosaccharide, and constructs and compositions for use in such methods.

公开(公告)号：US20180153983A1

公开(公告)日：2018-06-07

申请号：US15809524

申请日：2017-11-10

Applicant: GLAXOSMITHKLINE BIOLOGICALS, SA

Inventor： Francesco BERTI ,  Paolo COSTANTINO ,  Maria Rosaria ROMANO

IPC: A61K39/385 ,  A61K47/64 ,  A61K39/085 ,  A61K39/00

Abstract: The invention provides a process for preparing a conjugate of a S.aureus type 5 or type 8 capsular polysaccharide and a carrier molecule, comprising the steps of: (a) depolymerising the capsular polysaccharide, to give a polysaccharide fragment; (b) oxidising the fragment in order to introduce an aldehyde group into at least one saccharide residue in the fragment, to give an oxidised saccharide residue; and (c) coupling the oxidised saccharide residue to a carrier molecule via the aldehyde group, thereby giving the conjugate. The coupling in step (c) may be direct, or may be via a linker molecule. The invention also provides a conjugate obtained or obtainable by this process.

公开(公告)号：US20220089627A1

公开(公告)日：2022-03-24

申请号：US17528426

申请日：2021-11-17

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Paolo COSTANTINO ,  Maria Rosaria ROMANO ,  Francesco BERTI

IPC: C07H1/08 ,  C12P19/04 ,  A61K39/085

Abstract: The invention provides a method for releasing capsular polysaccharide from S. aureus type 5 or type 8 cells, comprising the step of treating the cells with acid. The invention further provides a process for purifying capsular polysaccharide from S. aureus type 5 or type 8 cells comprising this method. Other processing steps may be included in the process, such as enzymatic treatment, e.g. to remove nucleic acid, protein and/or peptidoglycan contaminants; diafiltration, e.g. to remove low molecular weight contaminants; anion exchange chromatography, e.g. to remove residual protein; and concentration.

公开(公告)号：US20170348408A1

公开(公告)日：2017-12-07

申请号：US15536132

申请日：2015-12-17

Applicant: GLAXOSMITHKLINE BIOLOGICALS SA

Inventor： Francesco BERTI ,  Paolo COSTANTINO ,  Maria Rosaria ROMANO

IPC: A61K39/09 ,  C08B37/00

CPC classification number: A61K39/092 ,  C08B37/0003 ,  C08B37/006 ,  C08B37/0063

Abstract: A purification method for the capsular polysaccharide of type II GBS in which the capsular polysaccharide is filtered using a membrane with a cut-off of less than 30 kDa.