公开(公告)号：US4835105A

公开(公告)日：1989-05-30

申请号：US71577

申请日：1987-07-09

申请人： Gabor Seres ,  Miklos Jarai ,  Sandor Piukovich ,  Maria Szigetvari nee Gabanyi ,  Jozsef Szejtli

发明人： Gabor Seres ,  Miklos Jarai ,  Sandor Piukovich ,  Maria Szigetvari nee Gabanyi ,  Jozsef Szejtli

IPC分类号： C12P19/18 ,  C08B37/16

CPC分类号： C08B37/0012

摘要： The invention relates to a process for the preparation of high-purity gamma- and alpha-cyclodextrin starting from a starch substrate of low reducing capacity, partially hydrolysed with alpha-amylase. According to the invention a starch substrate of low reducing capacity partially hydrolysed with alpha-amylase is converted with cyclodextrin glucosyl transferase, after the addition of an aliphatic ketone of the formula R.sub.1 COR.sub.2, in which R.sub.1 and R.sub.2 stand for a straight or branched chained alkyl group having from 1 to 6 carbon atoms, and/or a phenol derivative of the formula R.sub.3 R.sub.4 PheOH, wherein R.sub.3 and R.sub.4 are hydrogen, alkyl having from 1 to 4 carbon atoms, or R.sub.3 and R.sub.4 together form phenyl group, Phe stand for phenyl group, OH is hydroxyl and/or a benzene derivative of the formula R.sub.5 R.sub.6 Phe, wherein R.sub.5 and R.sub.6 are hydrogen, alkyl having from 1 to 4 carbon atoms, or R.sub.5 and R.sub.6 together form phenyl group, Phe is phenyl, as a precipitating agent, and if desired, alpha- and/or gamma-cyclodextrin is isolated from the conversion mixture in a crude form, or after further purification, in a pure form.

摘要翻译： 本发明涉及从低还原能力的淀粉底物开始制备高纯度γ-和α-环糊精的方法，其部分用α-淀粉酶水解。 根据本发明，在加入式R 1 COR 2的脂族酮后，用环糊精葡糖基转移酶转化用α-淀粉酶部分水解的低还原能力的淀粉底物，其中R 1和R 2表示直链或支链链烷基 具有1至6个碳原子，和/或式R3R4PheOH的酚衍生物，其中R3和R4是氢，具有1至4个碳原子的烷基，或R3和R4一起形成苯基，Phe代表苯基， OH是羟基和/或式R 5 R 6 Phe的苯衍生物，其中R 5和R 6是氢，具有1至4个碳原子的烷基或R 5和R 6一起形成苯基，Phe是苯基，作为沉淀剂，如果 所需的α-和/或γ-环糊精以粗制形式从转化混合物中分离，或在进一步纯化后以纯形式分离。

公开(公告)号：US4593004A

公开(公告)日：1986-06-03

申请号：US526034

申请日：1983-08-24

申请人： Laszlo Boross ,  Ivan Daroczi ,  Katalin Ivony nee Kaldive ,  Gabor Seres ,  Bela Szanjani ,  Jozsef Szejtli

发明人： Laszlo Boross ,  Ivan Daroczi ,  Katalin Ivony nee Kaldive ,  Gabor Seres ,  Bela Szanjani ,  Jozsef Szejtli

IPC分类号： C12N11/08 ,  C12N11/10 ,  C12P19/18 ,  C12N11/06 ,  C12N11/02

CPC分类号： C12P19/18 ,  C12N11/08 ,  C12N11/10

摘要： The invention relates to a process for the preparation of a cyclodextrine glycosyltransferase enzyme.The immobilization can be carried out by two methods. According to the first method a cyclodextrine glycosyltransferase enzyme is activated with a solution of a carbodiimide compound and the thus treated enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polysaccharide derivative comprising at least 0.1 m-equiv./g of free amino group. According to the second method the enzyme is applied in a solution having a pH value of 4.5-8.5 onto a polymer produced from an acrylic acid and/or methacrylic acid or acrylic amide and/or methacrylic amide monomer by means of a cross-linking agent of the acrylic or allylic type which polymer comprises at least 0.1 m-equiv./g of functional carboxy group, activating the polymer with a solution of a carbodiimide compound and washing and if desired drying the product thus obtained.The advantage of the process of the present invention is that the thus immobilized enzyme--contrary to the dissolved enzyme--is suitable for longlasting application and can be readily used on industrial scale continuous production.

摘要翻译： 本发明涉及一种制备环糊精糖基转移酶的方法。 固定化可以通过两种方法进行。 根据第一种方法，环糊精糖基转移酶用碳二亚胺化合物的溶液活化，将这样处理过的酶在pH值为4.5-8.5的溶液中施用到包含至少0.1m-准/克 的游离氨基。 根据第二种方法，将酶以pH值为4.5-8.5的溶液施用到由丙烯酸和/或甲基丙烯酸或丙烯酰胺和/或甲基丙烯酰胺单体通过交联剂产生的聚合物上 的丙烯酸或烯丙基类型，该聚合物包含至少0.1m比例的功能性羧基，用碳二亚胺化合物溶液活化聚合物，并且如果需要，干燥所得到的产物。 本发明方法的优点是与溶解的酶相反的固定化酶适用于长时间应用，并且可以容易地用于工业规模的连续生产。